Tanaman Indikator Dan Teknik Rapd-PCR Untuk Penentuan Biotipe Bemisia Tabaci Gennadius (Hemiptera: Aleyrodidae)

Abstract

Indicator Plant and PCR-RAPD for Biotype Determination of Bemisia tabaci Gennadius (Hemiptera: Aleyrodidae).B. tabaci has been known world wide as a major pest and virus vector of horticulture. In Indonesia the presence of B.tabaci was reported since 1980 and its role as virus vector in tomato and chilli pepper has becoming more importantrecently. Genetic diversity of B. tabaci has been well recognized, but very little information available for diversity of B.tabaci in Indonesia. This research was conducted in Bogor, West Java from May 2004 to June 2005. The aim of thisresearch was to initiate basic information regarding genetic diversity of B. tabaci in Indonesia, particularly in Java Island.Whiteflies population collected from different crops, i.e. tomato, broccoli, chill pepper, eggplant, cucumber, soybean, andedamame, was evaluated using silverleaf-induction test, and RAPD-PCR. It was evidenced that only B. tabaci populationfrom broccoli was able to induce silverleaf. Two genetic types of B. tabaci, i.e. biotype B and non B, were identified basedon polymorphism character of DNA. Population from broccoli was belong to biotype B, whereas other populations fromtomato, chill pepper, eggplant, cucumber, soybean, and edamame were belong to biotype non B.

Molecular Analysis of Cytochrome Oxidase I in Bemisia tabaci (Gennadius) Populations Collected from Four Districts in the Special Region of Yogyakarta

“…Several studies have been done to identify genetic characteristics of B. tabaci in Indonesia. Hidayat et al (2008) detected B (MEAM1) dan non-B B. tabaci biotypes by using PCR. Another study analyzed COI sequence and found different native genotypes in Indonesia, including Asia I, Asia II 5, Asia II 6, Asia II 7, Asia II 12, and Australia/Indonesia.…”