Objective: To evaluate the effect of Absorbable Gelatin Sponge (AGS) and Colla cote as a biological barrier in pulpectomized primary anterior teeth. Subjects and methods: This study was undertaken on 36 primary anterior teeth indicated for pulpectomy. The teeth were classified randomly according to the used obturation materials (Metapex, Metapex/AGS, and Metapex/Colla Cote) into 3 equal groups. All teeth were evaluated clinical and radiographic to assess the quality of obturation. Results: All pulpectomized teeth in Metapex and Metapex/Colla Cote groups, showed materials extrusion beyond the apex at immediate postoperative. While after three months of follow-up, all extruded materials beyond the apex were resorbed. However, all teeth in Metapex/AGS group, at immediate and three months showed no extrusion beyond the apex. Conclusion: The addition of AGS to Metapex can increase the viscosity of the Metapex, which in turn decreases the tendency of Metapex extrusion and post-operative periapical inflammation and pain, thus increasing the success rate chances.