Tamoxifen-loaded solid lipid nanoparticles-induced apoptosis in breast cancer cell lines

Abbasalipourkabir, Roghayeh; Salehzadeh, Aref; Abdullah, Rasedee

doi:10.1080/17458080.2015.1038660

Cited by 33 publications

(14 citation statements)

References 29 publications

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“…In fact, apoptosis need not always be preceded by cell cycle arrest. For example, ethanol extract of the microalga Chaetoceros calcitrans , 31 tamoxifen-loaded solid lipid nanoparticles, 32 and avasimibe 33 can kill cells without halting their cell cycle. We then investigated the nature of the cell death.…”

Section: Discussionmentioning

confidence: 99%

Proteomic and metabolomic profiling combined with in vitro studies reveal the antiproliferative mechanism of silver nanoparticles in MDA-MB-231 breast carcinoma cells

2022

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Section: Discussionmentioning

confidence: 99%

Proteomic and metabolomic profiling combined with in vitro studies reveal the antiproliferative mechanism of silver nanoparticles in MDA-MB-231 breast carcinoma cells

2022

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“…Sun et al (2014) demonstrated that QT-nanostructured lipid carriers markedly enhanced the QT solubility and stability and increased the QT content in MCF-7 cells. In their study, the enhanced cytotoxicity was parallel to increased QT uptake by MCF-7 cells [44].…”

Section: Discussionmentioning

confidence: 99%

Effects of Quercetin-Loaded Nanoparticles on MCF-7 Human Breast Cancer Cells

et al. 2019

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: Background and objectives: Previous studies have shown anti-tumor activity of quercetin (QT). However, the low bioavailability of QT has restricted its use. This study aimed to assess the toxic effect of QT encapsulated in solid lipid nanoparticles (QT-SLNs) on the growth of MCF-7 human breast cancer cells. Materials and Methods: MCF-7 and MCF-10A (non-tumorigenic cell line) cell lines treated with 25 µmol/mL of QT or QT-SLNs for 48 h. Cell viability, colony formation, oxidative stress, and apoptosis were evaluated to determine the toxic effects of the QT-SLNs. Results: The QT-SLNs with appropriate characteristics (particle size of 85.5 nm, a zeta potential of −22.5 and encapsulation efficiency of 97.6%) were prepared. The QT-SLNs showed sustained QT release until 48 h. Cytotoxicity assessments indicated that QT-SLNs inhibited MCF-7 cells growth with a low IC50 (50% inhibitory concentration) value, compared to the free QT. QT-SLNs induced a significant decrease in the viability and proliferation of MCF-7 cells, compared to the free QT. QT-SLN significantly increased reactive oxygen species (ROS) level and MDA contents and significantly decreased antioxidant enzyme activity in the MCF-7 cells. Following QT-SLNs treatment, the expression of the Bcl-2 protein significantly decreased, whereas Bx expression showed a significant increase in comparison with free QT-treated cells. Furthermore, The QT-SLNs significantly increased apoptotic and necrotic indexes in MCF-7 cells. Viability, proliferation, oxidative stress and apoptosis of MCF-10A cells were not affected by QT or QT-SLNs. Conclusion: According to the results of this study, SLN significantly enhanced the toxic effect of QT against human breast cancer cells.

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“…Tamoxifen is a potent hydrophobic endocrine drug commonly used to treat breast cancers and patients at high risk (Day et al, 2020 ; Abbasalipourkabir et al, 2016 ). Tamoxifen can act as estrogenic or antiestrogenic, depending on the dosage and the tissues targeted ( Figure 1(A) ).…”

Section: Introductionmentioning

confidence: 99%

Formulation, characterization, and cellular toxicity assessment of tamoxifen-loaded silk fibroin nanoparticles in breast cancer

Moin

Wani²,

Osmani

et al. 2021

Drug Delivery

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Silk fibroin (SF) is a natural polymeric biomaterial that is widely adopted for the preparation of drug delivery systems. Herein, we aimed to fabricate and characterize SF nanoparticles loaded with the selective estrogen receptor modulator; tamoxifen citrate (TC-SF-NPs) and to assess their in vitro efficacy against breast cancer cell lines (MCF-7 and MDA-MB-231). TC-loaded SF-NPs were characterized for particle size, morphology, entrapment efficiency, and release profile. In addition, we examined the in vitro cytotoxicity of TC-SF-NPs against human breast cancer cell lines and evaluated the anticancer potential of TC-SF-NPs through apoptosis assay and cell cycle analysis. Drug-loaded SF-NPs showed an average particle size of 186.1 ± 5.9 nm and entrapment efficiency of 79.08%. Scanning electron microscopy (SEM) showed the nanoparticles had a spherical morphology with smooth surface. Tamoxifen release from SF-NPs exhibited a biphasic release profile with an initial burst release within the first 6 h and sustained release for 48 h. TC-SF-NPs exerted a dose-dependent cytotoxic effect against breast cancer cell lines. In addition, flow cytometry analysis revealed that cells accumulate in G 0 /G 1 phase, with a concomitant reduction of S- and G 2 -M-phase cells upon treatment with TC-SF-NPs. Consequently, the potent anticancer activities of TC-SF-NPs against breast cancer cells were mainly attributed to the induction of apoptosis and cell cycle arrest. Our results indicate that SF nanoparticles may represent an attractive nontoxic nanocarrier for the delivery of anticancer drugs.

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Tamoxifen-loaded solid lipid nanoparticles-induced apoptosis in breast cancer cell lines

Cited by 33 publications

References 29 publications

Proteomic and metabolomic profiling combined with in vitro studies reveal the antiproliferative mechanism of silver nanoparticles in MDA-MB-231 breast carcinoma cells

Proteomic and metabolomic profiling combined with in vitro studies reveal the antiproliferative mechanism of silver nanoparticles in MDA-MB-231 breast carcinoma cells

Effects of Quercetin-Loaded Nanoparticles on MCF-7 Human Breast Cancer Cells

Formulation, characterization, and cellular toxicity assessment of tamoxifen-loaded silk fibroin nanoparticles in breast cancer

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