TaLHY, a 1R-MYB Transcription Factor, Plays an Important Role in Disease Resistance against Stripe Rust Fungus and Ear Heading in Wheat

Abstract

LHY (late elongated hypocotyl) is an important gene that regulates and controls biological rhythms in plants. Additionally, LHY is highly expressed in the SSH (suppression subtractive hybridization) cDNA library-induced stripe rust pathogen (CYR32) in our previous research. To identify the function of the LHY gene in disease resistance against stripe rust, we used RACE-PCR technology to clone TaLHY in the wheat variety Chuannong19. The cDNA of TaLHY is 3085 bp long with an open reading frame of 1947 bp. TaLHY is speculated to encode a 70.3 kDa protein of 648 amino acids , which has one typical plant MYB-DNA binding domain; additionally, phylogenetic tree shows that TaLHY has the highest homology with LHY of Brachypodium distachyon(BdLHY-like). Quantitative fluorescence PCR indicates that TaLHY has higher expression in the leaf, ear and stem of wheat but lower expression in the root. Infestation of CYR32 can result in up-regulated expression of TaLHY, peaking at 72 h. Using VIGS (virus-induced gene silencing) technology to disease-resistant wheat in the fourth leaf stage, plants with silenced TaLHY cannot complete their heading stage. Through the compatible interaction with the stripe rust physiological race CYR32, Chuannong 19 loses its immune capability toward the stripe rust pathogen, indicating that TaLHY may regulate and participate in the heading of wheat, as well as the defense responses against stripe rust infection.

Identification of an orthologous clade of peroxidases that respond to feeding by greenbugs (Schizaphis graminum) in C4 grasses

“…Further, no novel enriched motifs were detected in the aphid responsive genes using DREME and no motifs that were exclusively associated with the aphid-induced peroxidases could be identified; however, MYB2CONSENSUSAT and CURECORECR motifs were highly abundant in the promoters of the orthologue group of aphid responsive genes Sobic.002G416600, Pavir.Ba00167 and Si030339 and may be linked to their activation under periods of biotic stress. Several myb transcription factors induce gene expression under periods of biotic stress in grasses and activate networks of defencerelated pathways (Ibraheem et al 2015;Zhang et al 2015) and CURECORECR motifs are responsive to changes in oxygen levels and may be triggered by changes in ROS levels that often accompany insect feeding. Other regulatory elements that might induce the expression of all or a subset of the peroxidases in this interval include long range promoters or enhancers (Sanyal et al 2012).…”

Different responses of photosystem and antioxidant defense system to three environmental stresses in wheat seedlings

“…For qRT-PCR analysis, specific primers of eight stress-responsive genes (TaMYB73,TaABC1,TaOPR1,TaASR1,TaWRKY44,TaWRKY2,TaWRKY19,and TaCIPK29) were synthesized (Table 2S, supplement). Stress-associated genes in wheat plants were analyzed by qRT-PCR according to the method of Zhang et al (2015). SYBR Premix Ex Taq TM II Master (TaKaRa, Japan) was used for quantitative gene expression assays.…”

Effects of Stripe Rust Infection on the Levels of Redox Balance and Photosynthetic Capacities in Wheat

“…This suggested that these disease resistance-related enzymes possibly contribute to greater disease resistance in the incompatible. As two important disease resistance genes in wheat, TaNIT and TaLHY may participate in regulating the defense response against stripe rust infection [47,48]. A previous study indicated that the expression of TaLHY was up-regulated after infection by CYR32 in Chuannong 19 with virus-induced gene silencing [48].…”

“…As two important disease resistance genes in wheat, TaNIT and TaLHY may participate in regulating the defense response against stripe rust infection [47,48]. A previous study indicated that the expression of TaLHY was up-regulated after infection by CYR32 in Chuannong 19 with virus-induced gene silencing [48]. We showed that infection by Pst could result in the up-regulated expression of TaNIT and TaLHY in CM42 (Figure 6), which suggests that these two genes may be involved in the defense response to stripe rust in wheat.…”