TALE nickase-mediated<i>SP110</i>knockin endows cattle with increased resistance to tuberculosis

Wu, Haibo; Wang, Yongsheng; Zhang, Yan; Yang, Mingqi; Lv, Jiaxing; Li, Jun; Zhang, Yong

doi:10.1073/pnas.1421587112

Cited by 158 publications

(115 citation statements)

References 62 publications

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“…Zinc finger nickases and transcription activator-like effector (TALE) nickases have been made by generating a D450A mutation in the FokI nuclease domain, but these nickases appear to have noticeable residual nuclease activity (Ramirez et al, 2012;Wang et al, 2012;Wu et al, 2015). On the contrary, either of the two endonuclease domains of Cas9, HNH, and RuvC can be mutated to form nickases (Gasiunas et al, 2012;Jinek et al, 2012).…”

mentioning

confidence: 99%

A CRISPR/Cas9 Toolbox for Multiplexed Plant Genome Editing and Transcriptional Regulation

et al. 2015

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The relative ease, speed, and biological scope of clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPRassociated Protein9 (Cas9)-based reagents for genomic manipulations are revolutionizing virtually all areas of molecular biosciences, including functional genomics, genetics, applied biomedical research, and agricultural biotechnology. In plant systems, however, a number of hurdles currently exist that limit this technology from reaching its full potential. For example, significant plant molecular biology expertise and effort is still required to generate functional expression constructs that allow simultaneous editing, and especially transcriptional regulation, of multiple different genomic loci or multiplexing, which is a significant advantage of CRISPR/Cas9 versus other genome-editing systems. To streamline and facilitate rapid and wide-scale use of CRISPR/Cas9-based technologies for plant research, we developed and implemented a comprehensive molecular toolbox for multifaceted CRISPR/Cas9 applications in plants. This toolbox provides researchers with a protocol and reagents to quickly and efficiently assemble functional CRISPR/Cas9 transfer DNA constructs for monocots and dicots using Golden Gate and Gateway cloning methods. It comes with a full suite of capabilities, including multiplexed gene editing and transcriptional activation or repression of plant endogenous genes. We report the functionality and effectiveness of this toolbox in model plants such as tobacco (Nicotiana benthamiana), Arabidopsis (Arabidopsis thaliana), and rice (Oryza sativa), demonstrating its utility for basic and applied plant research.

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confidence: 99%

A CRISPR/Cas9 Toolbox for Multiplexed Plant Genome Editing and Transcriptional Regulation

et al. 2015

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“…Many considered uses of the technology are the same as those already considered in current breeding programs including production, health, and welfare, but with targeted efforts on known causative variants. Examples of edited dairy cattle include cattle with increased resistance to tuberculosis (Wu et al, 2015), the knockout of the betalactoglobulin gene , and enhanced mastitis resistance (Liu et al, 2013(Liu et al, , 2014. A promising first use of gene editing in the dairy industry to address welfare issues may be the production of hornless dairy cattle with the introduction of the POLLED allele, which is nearly fixed in some beef breeds but low in frequency in Holsteins (Carlson et al, 2016).…”

Section: Genome Editingmentioning

confidence: 99%

Invited review: Reproductive and genomic technologies to optimize breeding strategies for genetic progress in dairy cattle

Fleming¹,

Abdalla²,

Maltecca

et al. 2018

Arch. Anim. Breed.

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Abstract. Dairy cattle breeders have exploited technological advances that have emerged in the past in regards to reproduction and genomics. The implementation of such technologies in routine breeding programs has permitted genetic gains in traditional milk production traits as well as, more recently, in low-heritability traits like health and fertility. As demand for dairy products increases, it is important for dairy breeders to optimize the use of available technologies and to consider the many emerging technologies that are currently being investigated in various fields. Here we review a number of technologies that have helped shape dairy breeding programs in the past and present, along with those potentially forthcoming. These tools have materialized in the areas of reproduction, genotyping and sequencing, genetic modification, and epigenetics. Although many of these technologies bring encouraging opportunities for genetic improvement of dairy cattle populations, their applications and benefits need to be weighed with their impacts on economics, genetic diversity, and society.

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“…The advent of engineered endonucleases (EENs), including zinc finger nucleases (ZFNs) [151], transcription activator-like effector nucleases (TALENs) [152] and clustered regularly interspaced short palindromic repeats (CRISPR/Cas9) [153]), allows to cut a specific position in DNA sequence and then use endogenous cellular pathways to direct DNA repair to introduce specified alterations to the DNA sequence. Genome-editing approaches have been successfully used in different livestock species, such as pig [154,155], goat [156], cattle [157] and sheep [158]. In dairy cows, these technologies have been used to manipulate the genome so that they produce specific milk types, such as milk that causes less allergic problems (e.g.…”

Section: Genome Editing Technology and Non-coding Rnamentioning

confidence: 99%

Non-Coding RNA Roles in Ruminant Mammary Gland Development and Lactation

Ngoc¹,

Ibeagha‐Awemu²

2017

Current Topics in Lactation

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The ruminant mammary gland (MG) is an important organ charged with the production of milk for young and human nourishment. ) are a class of untranslated RNA molecules that function to regulate gene expression, associated biochemical pathways and cellular functions and are involved in many biological processes. This chapter presents a review of the current state of knowledge on the role of ncRNAs (particularly miRNAs and lncRNAs) in the MG and lactation processes, lactation signalling pathways, lipid metabolism, MG health of ruminants as well as miRNA roles in milk recipients. Finally, the potential application of new genome editing technology for ncRNA studies in MG development, the lactation process and milk components is presented.

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TALE nickase-mediatedSP110knockin endows cattle with increased resistance to tuberculosis

Cited by 158 publications

References 62 publications

A CRISPR/Cas9 Toolbox for Multiplexed Plant Genome Editing and Transcriptional Regulation

A CRISPR/Cas9 Toolbox for Multiplexed Plant Genome Editing and Transcriptional Regulation

Invited review: Reproductive and genomic technologies to optimize breeding strategies for genetic progress in dairy cattle

Non-Coding RNA Roles in Ruminant Mammary Gland Development and Lactation

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