Taking a bite out of spinal cord injury: do dental stem cells have the teeth for it?

Bianco, John; Berdt, Pauline De; Deumens, Ronald; Rieux, Anne des

doi:10.1007/s00018-015-2126-5

Cited by 25 publications

(25 citation statements)

References 288 publications

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“…The cascade of secondary neuro-degenerative events following spinal cord injury (SCI) includes bleeding, necrotic or apoptotic processes and lesion area enlargement [1,2].…”

Section: Introductionmentioning

confidence: 99%

Stem cells from human apical papilla decrease neuro-inflammation and stimulate oligodendrocyte progenitor differentiation via activin-A secretion

Berdt

Bottemanne

Bianco

et al. 2018

Cell. Mol. Life Sci.

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Secondary damage following spinal cord injury leads to non-reversible lesions and hampering of the reparative process. The local production of pro-inflammatory cytokines such as TNF-α can exacerbate these events. Oligodendrocyte death also occurs, followed by progressive demyelination leading to significant tissue degeneration. Dental stem cells from human apical papilla (SCAP) can be easily obtained at the removal of an adult immature tooth. This offers a minimally invasive approach to re-use this tissue as a source of stem cells, as compared to biopsying neural tissue from a patient with a spinal cord injury. We assessed the potential of SCAP to exert neuroprotective effects by investigating two possible modes of action: modulation of neuro-inflammation and oligodendrocyte progenitor cell (OPC) differentiation. SCAP were co-cultured with LPS-activated microglia, LPS-activated rat spinal cord organotypic sections (SCOS), and LPS-activated co-cultures of SCOS and spinal cord adult OPC. We showed for the first time that SCAP can induce a reduction of TNF-α expression and secretion in inflamed spinal cord tissues and can stimulate OPC differentiation via activin-A secretion. This work underlines the potential therapeutic benefits of SCAP for spinal cord injury repair.

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“…The cascade of secondary neuro-degenerative events following spinal cord injury (SCI) includes bleeding, necrotic or apoptotic processes and lesion area enlargement [1,2].…”

Section: Introductionmentioning

confidence: 99%

Stem cells from human apical papilla decrease neuro-inflammation and stimulate oligodendrocyte progenitor differentiation via activin-A secretion

Berdt

Bottemanne

Bianco

et al. 2018

Cell. Mol. Life Sci.

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“…Remarkably, dental pulp MSCs express stemness-related markers as Oct-3/4, Nanog, and sex-determining region Y-(SRY-) box 2 (SOX-2) [134], in addition to a variety of angiogenic factors such as VEGF, PDGF, and FGF, with an interesting increase of their expression after injury [135], as well as CSF, IL-8, angiogenin, endothelin-1, angiopoietin-1, and IGF-binding protein-3 [136][137][138]. Dental pulp MSCs demonstrate immunomodulatory properties partly attributable to their expression of IL-8, IL-6, and TGF-β, which could inhibit T cell function [139,140]. Moreover, dental pulp MSCs secrete many neurotrophic factors like BDNF [141], glial cell linederived neurotrophic factor [142], and NGF [143].…”

Section: Dental Pulp Mesenchymal Stem/progenitormentioning

confidence: 99%

Dental Stem Cell-Derived Secretome/Conditioned Medium: The Future for Regenerative Therapeutic Applications

Moshy

Radwan

Rady

et al. 2020

Stem Cells International

108

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Regenerative medicine literature has proposed mesenchymal stem/progenitor cell- (MSC-) mediated therapeutic approaches for their great potential in managing various diseases and tissue defects. Dental MSCs represent promising alternatives to nondental MSCs, owing to their ease of harvesting with minimally invasive procedures. Their mechanism of action has been attributed to their cell-to-cell contacts as well as to the paracrine effect of their secreted factors, namely, secretome. In this context, dental MSC-derived secretome/conditioned medium could represent a unique cell-free regenerative and therapeutic approach, with fascinating advantages over parent cells. This article reviews the application of different populations of dental MSC secretome/conditioned medium in in vitro and in vivo animal models, highlights their significant implementation in treating different tissue’ diseases, and clarifies the significant bioactive molecules involved in their regenerative potential. The analysis of these recent studies clearly indicate that dental MSCs’ secretome/conditioned medium could be effective in treating neural injuries, for dental tissue regeneration, in repairing bone defects, and in managing cardiovascular diseases, diabetes mellitus, hepatic regeneration, and skin injuries, through regulating anti-inflammatory, antiapoptotic, angiogenic, osteogenic, and neurogenic mediators.

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“…MSCs can migrate to areas of injury [1] and integrate with damaged tissues, where they mediate immunomodulatory [2][3][4], antiapoptotic, and anti-inflammatory effects through the secretion of various neurotrophic factors and cytokines and signaling pathway activation through specific receptors on target cells [5]. These specific features, along with multiple studies reporting the ability of MSCs to promote regeneration of nervous tissues in the brain and spinal cord, support the potential of MSCs as treatments of neurodegenerative conditions and traumatic brain and spinal cord injury (SCI) [6,7].…”

Section: Introductionmentioning

confidence: 95%

Mesenchymal Stem Cell Therapy for Spinal Cord Contusion: A Comparative Study on Small and Large Animal Models

et al. 2019

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Here, we provide a first comparative study of the therapeutic potential of allogeneic mesenchymal stem cells derived from bone marrow (BM-MSCs), adipose tissue (AD-MSCs), and dental pulp (DP-MSCs) embedded in fibrin matrix, in small (rat) and large (pig) spinal cord injury (SCI) models during subacute period of spinal contusion. Results of behavioral, electrophysiological, and histological assessment as well as immunohistochemistry and real-time polymerase chain reaction analysis suggest that application of AD-MSCs combined with a fibrin matrix within the subacute period in rats (2 weeks after injury), provides significantly higher post-traumatic regeneration compared to a similar application of BM-MSCs or DP-MSCs. Within the rat model, use of AD-MSCs resulted in a marked change in: (1) restoration of locomotor activity and conduction along spinal axons; (2) reduction of post-traumatic cavitation and enhancing tissue retention; and (3) modulation of microglial and astroglial activation. The effect of an autologous application of AD-MSCs during the subacute period after spinal contusion was also confirmed in pigs (6 weeks after injury). Effects included: (1) partial restoration of the somatosensory spinal pathways; (2) reduction of post-traumatic cavitation and enhancing tissue retention; and (3) modulation of astroglial activation in dorsal root entry zone. However, pigs only partially replicated the findings observed in rats. Together, these results indicate application of AD-MSCs embedded in fibrin matrix at the site of SCI during the subacute period can facilitate regeneration of nervous tissue in rats and pigs. These results, for the first time, provide robust support for the use of AD-MSC to treat subacute SCI.

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Taking a bite out of spinal cord injury: do dental stem cells have the teeth for it?

Cited by 25 publications

References 288 publications

Stem cells from human apical papilla decrease neuro-inflammation and stimulate oligodendrocyte progenitor differentiation via activin-A secretion

Stem cells from human apical papilla decrease neuro-inflammation and stimulate oligodendrocyte progenitor differentiation via activin-A secretion

Dental Stem Cell-Derived Secretome/Conditioned Medium: The Future for Regenerative Therapeutic Applications

Mesenchymal Stem Cell Therapy for Spinal Cord Contusion: A Comparative Study on Small and Large Animal Models

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