Take a good catch at the scat: carboxylic and sulfonic acid profiles as a non-invasive tool for species identification and sex determination in neotropical carnivores

Mastella, Ana Maria Obino; Rodrigues, Carlos Eduardo; Kist, Tarso B. Ledur; Pereira, María João Ramos

doi:10.1080/01650521.2021.1994786

Cited by 2 publications

(4 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2012; Mastella et al . 2021); glycocholic acid, found in pumas and jaguars (Cazón et al . 2009; Salame‐Méndez et al .…”

Section: Discussionmentioning

confidence: 99%

“…In fact, several of the bile acids found are shared with other mammals, including cholic acid, found in canids, felids, and procyonids (Guerrero et al 2006;Cazón et al 2009;Salame-Méndez et al 2012;Mastella et al 2021); glycocholic acid, found in pumas and jaguars (Cazón et al 2009;Salame-Méndez et al 2012;Nasini et al 2013); and taurocholic acid, found in anteaters and armadillos (Araujo et al 2010). So, multitaxon studies are necessary to stipulate peak intensity patterns for terrestrial mammals, to understand which bile acids have discriminatory power and at which taxonomic level.…”

Section: Discussionmentioning

confidence: 99%

“…Sample processing followed 4 to 5 cycles of the following major steps: (1) 4 h in an oven at 60°C; (2) 1 h in the hood; (3) determination of the weight to the nearest 0.1 mg per cycle (Hinnant & Kothmann 1988; Mastella et al . 2021). We repeated all steps until the samples reached constant mass.…”

Section: Methodsmentioning

confidence: 99%

“…(3) determination of the weight to the nearest 0.1 mg per cycle (Hinnant & Kothmann 1988;Mastella et al 2021). We repeated all steps until the samples reached constant mass.…”

Section: Sample Collection and Preparationmentioning

confidence: 99%

See 3 more Smart Citations

Neotropical mustelids: fecal metabolome diversity and its potential for taxonomic discrimination

et al. 2022

Self Cite

View full text Add to dashboard Cite

Chemical profiles of non‐invasive biological material, such as feces, have great potential to study elusive animals or those with low population densities. Here, we use a metabolomic approach to evaluate Neotropical mustelids as a biological model to describe the diversity of the metabolites present in fecal samples, as well as to evaluate the potential of chemical profiles for taxonomic discrimination. We collected fecal samples from captive individuals of 5 species of mustelids occurring in Brazil and analyzed them by liquid chromatography coupled to high‐resolution mass spectrometry. Over 200 compounds have been annotated; “bile acids, alcohols and derivatives” was the most expressive class in the metabolome of all the species. We successfully discriminated 3 taxonomic groups: 1—tayra (Eira barbara); 2—otters (Lontra longicaudis and Pteronura brasiliensis; 1); and 3—grisons (Galictis vittata and Galictis cuja). Several compounds seemed to be associated with food intake and the digestive process, while others were found for the first time in Neotropical mustelids. We concluded that mustelids show high metabolome diversity and that species‐specific identification through metabolomic profiles is possible, thus contributing to the development and implementation of additional non‐invasive approaches in the study of mustelids.

show abstract

“…2012; Mastella et al . 2021); glycocholic acid, found in pumas and jaguars (Cazón et al . 2009; Salame‐Méndez et al .…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…(3) determination of the weight to the nearest 0.1 mg per cycle (Hinnant & Kothmann 1988;Mastella et al 2021). We repeated all steps until the samples reached constant mass.…”

Section: Sample Collection and Preparationmentioning

confidence: 99%

See 2 more Smart Citations

Neotropical mustelids: fecal metabolome diversity and its potential for taxonomic discrimination

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

Do fecal bile acid analyses allow taxonomic discrimination between Neotropical mustelids?

et al. 2023

View full text Add to dashboard Cite

A diferenciação taxonômica de espécies tem sido cada vez mais suportada por análises de amostras fecais e abordagens moleculares. A partir da análise semiquantitativa de ácidos biliares fecais de cinco mustelídeos neotropicais acessamos a diversidade destes compostos, avaliando seu potencial na discriminação taxonômica de espécies. Amostras fecais coletadas de espécimes cativos foram analisadas por cromatografia líquida acoplada a espectrômetro de massas de alta performance. Diferenças significativas em quatro ácidos biliares foram detectadas entre as espécies analisadas. Os ácidos biliares são compostos endógenos fundamentais em vias bioquímicas, assim detectar diferenças interespecíficas pode ser relevante para o conhecimento da fisiologia, estado de saúde e respostas metabólicas dos mustelídeos às mudanças ambientais.

show abstract

Take a good catch at the scat: carboxylic and sulfonic acid profiles as a non-invasive tool for species identification and sex determination in neotropical carnivores

Cited by 2 publications

References 52 publications

Neotropical mustelids: fecal metabolome diversity and its potential for taxonomic discrimination

Neotropical mustelids: fecal metabolome diversity and its potential for taxonomic discrimination

Do fecal bile acid analyses allow taxonomic discrimination between Neotropical mustelids?

Contact Info

Product

Resources

About