Taiwan cobra phospholipase A2 suppresses ERK-mediated ADAM17 maturation, thus reducing secreted TNF-α production in human leukemia U937 cells

Chen, Ying Jung; Lin, Han; Chen, Ku Chung; Lin, Shinne Ren; Cheng, Tian−Lu; Chang, Long

doi:10.1016/j.toxicon.2014.05.012

Cited by 3 publications

(2 citation statements)

References 29 publications

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“…Lysophospholipids are important in cell signaling, phospholipid remodeling and membrane perturbation [ 19 ]. Some evidence indicated that PLA 2 hydrolyzed cellular membrane of various cancer cells [ 20 ]. In a study of membrane-interacted mode and catalytic activity, a PLA 2 of Naja naja atra was able to catalyze outer leaflet and inner leaflet of plasma membrane-mimicking vesicles [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

Anticancer properties of phospholipase A2 from Daboia siamensis venom on human skin melanoma cells

Khunsap

Khow

Buranapraditkun

et al. 2016

J Venom Anim Toxins Incl Trop Dis

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BackgroundPhospholipase A2 (PLA2) is a major component of the Daboia siamensis venom, which is able to hydrolyse the membrane of various cells. For this reason, the activity of PLA2 was investigated regarding its pharmaceutical properties. This study was conducted to explore the pharmacological properties of a PLA2 from Daboia siamensis (dssPLA2) venom on human skin melanoma cell line (SK-MEL-28).MethodsdssPLA2 was isolated by ion exchange and gel filtration columns. Various concentrations of dssPLA2 were investigated for cytotoxic activity and inhibition of migration on SK-MEL-28 cells. Cell death analysis, mRNA expression levels of Notch I-III and BRAF V600E genes were also determined.ResultsdssPLA2 exhibited cytotoxicity on SK-MEL-28 for 24 and 72 h as compared with untreated cells. However, it had no toxic effects on CCD-1064sk cells under the same conditions. dssPLA2 (0.25 and 0.5 μg/mL) induced 17.16 and 30.60 % of apoptosis, while activated 6.53 and 7.05 % of necrotic cells. dssPLA2 at 0.25, 0.5, 1 and 2 μg/mL could inhibit migration on SK-MEL-28 cells for 24 h by 31.06, 41.66, 50 and 68.75 %, respectively. The action of dssPLA2 significantly reduced the levels of Notch I and BRAF V600E genes expression on SK-MEL-28 cells compared with untreated cells at 72 h.ConclusionsThis study indicates that dssPLA2 had potential effects of apoptosis, necrosis, cytotoxicity and inhibition of migration on SK-MEL-28 cells. dssPLA2 could possibly be a selective agent that targets cancer cells without affecting normal cells.Electronic supplementary materialThe online version of this article (doi:10.1186/s40409-016-0061-z) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Anticancer properties of phospholipase A2 from Daboia siamensis venom on human skin melanoma cells

Khunsap

Khow

Buranapraditkun

et al. 2016

J Venom Anim Toxins Incl Trop Dis

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“…PLA 2 hydrolyzed phospholipid membrane producing lysophospholipid and free fatty acid, which caused cell damage [9,10]. PLA 2 has been studied against various cancer cells [11][12][13]. Previously, dssPLA 2 has been shown to inhibit melanoma cells which showed reduced BRAF V600E mRNA expression level in the MAPKs pathway without affected normal cell [14].…”

Section: Introductionmentioning

confidence: 99%

A phospholipase A2 of Daboia siamensis venom suppressed expression of genes and proteins in MAPK pathway

Khunsap¹,

Khow²,

Suntrarachun³

et al. 2020

ScienceAsia

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High mortality rate of Melanoma is due to poor response to radiotherapy and chemotherapy. A phospholipase A 2 (PLA 2) of Daboia siamensis venom has been reported to inhibit melanoma cells. The aim of this study is to evaluate the effects of dssPLA 2 on gene and protein expression of MAPKs (Mitrogen-activated protien kinases) in melanoma cells. The mRNA and protein were determined by quantitative real-time PCR and western blotting to detect the expression levels of MEK1, MEK2, BRAF V600E and ERK1/2. Results indicated that 0.25 µg/ml of dssPLA 2 reduced the mRNA expression level of these genes in a time dependent manner. Interestingly, at 48 h, the mRNA expression level of all genes was extremely decreased compared with untreated cells in the same condition. Whereas, the protein level of MEK1, MEK2, BRAF V600E and ERK1/2 were clearly reduced after dssPLA 2 incubation. Our results show that dssPLA 2 is a strong inhibitor to MAPK genes at 48 h.

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Emerging anticancer potential and mechanisms of snake venom toxins: A review

Guo,

Fu,

Peng

et al. 2024

International Journal of Biological Macromolecules

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Taiwan cobra phospholipase A2 suppresses ERK-mediated ADAM17 maturation, thus reducing secreted TNF-α production in human leukemia U937 cells

Cited by 3 publications

References 29 publications

Anticancer properties of phospholipase A2 from Daboia siamensis venom on human skin melanoma cells

Anticancer properties of phospholipase A2 from Daboia siamensis venom on human skin melanoma cells

A phospholipase A2 of Daboia siamensis venom suppressed expression of genes and proteins in MAPK pathway

Emerging anticancer potential and mechanisms of snake venom toxins: A review

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