Tailoring the stealth properties of biocompatible polysaccharide nanocontainers

Kang, Biao; Okwieka, Patricia; Schöttler, Susanne; Seifert, Oliver; Kontermann, Roland E.; Pfizenmaier, Klaus; Musyanovych, Anna; Meyer, Ralf G.; Diken, Mustafa; Şahin, Uğur; Mailänder, Volker; Wurm, Frederik R.; Landfester, Katharina

doi:10.1016/j.biomaterials.2015.01.042

Cited by 52 publications

(39 citation statements)

References 45 publications

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“…The protein-NP interaction is studied by incubating NPs in protein solutions for a period of time, either a single protein solution such as BSA, fibronectin and IgG, [11][12][13][14] or biological fluid containing multiple proteins such as serum, 15,16 plasma, cytosol, 17 fetal bovine serum (FBS), 18 fetal calf serum, synovial fluid, and cerebrospinal fluid. 19 Protein-NP complexes are then separated from the protein solution via instrumental analysis.…”

Section: Instrumental Analysis For Evaluating Protein Coronamentioning

confidence: 99%

Protein corona: a new approach for nanomedicine design

Nguyen¹,

Lee²

2017

IJN

522

343

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After administration of nanoparticle (NP) into biological fluids, an NP–protein complex is formed, which represents the “true identity” of NP in our body. Hence, protein–NP interaction should be carefully investigated to predict and control the fate of NPs or drug-loaded NPs, including systemic circulation, biodistribution, and bioavailability. In this review, we mainly focus on the formation of protein corona and its potential applications in pharmaceutical sciences such as prediction modeling based on NP-adsorbed proteins, usage of active proteins for modifying NP to achieve toxicity reduction, circulation time enhancement, and targeting effect. Validated correlative models for NP biological responses mainly based on protein corona fingerprints of NPs are more highly accurate than the models solely set up from NP properties. Based on these models, effectiveness as well as the toxicity of NPs can be predicted without in vivo tests, while novel cell receptors could be identified from prominent proteins which play important key roles in the models. The ungoverned protein adsorption onto NPs may have generally negative effects such as rapid clearance from the bloodstream, hindrance of targeting capacity, and induction of toxicity. In contrast, controlling protein adsorption by modifying NPs with diverse functional proteins or tailoring appropriate NPs which favor selective endogenous peptides and proteins will bring promising therapeutic benefits in drug delivery and targeted cancer treatment.

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Section: Instrumental Analysis For Evaluating Protein Coronamentioning

confidence: 99%

Protein corona: a new approach for nanomedicine design

Nguyen¹,

Lee²

2017

IJN

522

343

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“…After intravenous injection mannose nanocapsules are preferentially deposited in the lungs. 151 Despite the straightforward reaction setup, this strategy has limited feasibility, when used to encapsulate and protect pharmaceutical agents, which often contain nucleophiles like amines, thiols, or alcohols, and consequently will participate in the polycondensation reaction with the diisocyanate electrophile. 150 HES with a molecular weight of M w = 200 000 g mol À1 and a degree of substitution of 0.5 has also been used to prepare nanocapsules through the inverse miniemulsion technique.…”

Section: Carbohydrate-constructed Nanocarriersmentioning

confidence: 99%

Carbohydrate nanocarriers in biomedical applications: functionalization and construction

et al. 2015

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The specific targeting of either tumor cells or immune cells in vivo by carefully designed and appropriately surface-functionalized nanocarriers may become an effective therapeutic treatment for a variety of diseases. Carbohydrates, which are prominent biomolecules, have shown their outstanding ability in balancing the biocompatibility, stability, biodegradability, and functionality of nanocarriers. The recent applications of sugar (mono/oligosaccharides and/or polysaccharides) for the development of nanomedicines are summarized in this review, including the application of carbohydrates for the surface-functionalization of various nanocarriers and for the construction of the nanocarrier itself. Current problems and challenges are also addressed.

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“…The long half‐lives accessible by bioconjugation have led to the development of several potent commercially available PEGylated biologics . PEGylated nanocarriers have also achieved long in vivo half‐lives …”

Section: Introductionmentioning

confidence: 99%

Reversible Bioconjugation: Biodegradable Poly(phosphate)‐Protein Conjugates

Steinbach

Becker

Spiegel

et al. 2016

Macromolecular Bioscience

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Protein-polymer conjugates are widely used to improve the pharmacokinetic properties of therapeutic proteins. Commercially available conjugates employ poly(ethylene glycol) (PEG) as the protective polymer; however, PEG has a number of shortcomings, including non-biodegradability and immunogenicity, that call for the development of alternatives. Here, the synthesis of biodegradable poly(phosphate), that is, poly(ethyl ethylene phosphate) (PEEP), by organo-catalyzed anionic ring-opening polymerization exhibiting dispersity values Ð < 1.3 is reported. Polymers with molecular weights between 2000 and 33 200 g mol are then ω-functionalized with a succinimidyl carbonate group and subsequently conjugated to model proteins. These are the first conjugates based on polyphosphates which degraded upon exposure to phosphodiesterase. As is the case for PEGylated therapeutics, residual in vitro activity of the PPEylated conjugates depends on the extent of protein modification. These results suggest that PEEP exhibits the desired properties of a biopolymer for use in next generation, fully degradable drug delivery systems.

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Tailoring the stealth properties of biocompatible polysaccharide nanocontainers

Cited by 52 publications

References 45 publications

Protein corona: a new approach for nanomedicine design

Protein corona: a new approach for nanomedicine design

Carbohydrate nanocarriers in biomedical applications: functionalization and construction

Reversible Bioconjugation: Biodegradable Poly(phosphate)‐Protein Conjugates

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