Tailoring of the biochemical profiles of microalgae by employing mixotrophic cultivation

Smith, JK Penhaul; Hughes, A.D.; McEvoy, Lesley; Day, J.D.

doi:10.1016/j.biteb.2019.100321

Cited by 20 publications

(3 citation statements)

References 37 publications

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“…Glycerol can be used as a substrate by many algae, both in the light and in darkness (Perez‐Garcia et al ., 2011 ; Penhaul Smith et al ., 2020 ), and successful heterotrophic growth of coccolithophores was demonstrated in high concentrations of glycerol by Blankley ( 1971 ). We found here that C. neohelis was able to grow in low glycerol concentrations (Fig.…”

Section: Discussionmentioning

confidence: 99%

Osmotrophy of dissolved organic carbon by coccolithophores in darkness

2021

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Summary The evolutionary and ecological story of coccolithophores poses questions about their heterotrophy, surviving darkness after the end‐Cretaceous asteroid impact as well as survival in the deep ocean twilight zone. Uptake of dissolved organic carbon might be an alternative nutritional strategy for supply of energy and carbon molecules. Using long‐term batch culture experiments, we examined coccolithophore growth and maintenance on organic compounds in darkness. Radiolabelled experiments were performed to study the uptake kinetics. Pulse–chase experiments were used to examine the uptake into unassimilated, exchangeable pools vs assimilated, nonexchangeable pools. We found that coccolithophores were able to survive and maintain their metabolism for up to 30 d in darkness, accomplishing about one cell division. The concentration dependence for uptake was similar to the concentration dependence for growth in Cruciplacolithus neohelis, suggesting that it was taking up carbon compounds and immediately incorporating them into biomass. We recorded net incorporation of radioactivity into the particulate inorganic fraction. We conclude that osmotrophy provides nutritional flexibility and supports long‐term survival in light intensities well below threshold for photosynthesis. The incorporation of dissolved organic matter into particulate inorganic carbon, raises fundamental questions about the role of the alkalinity pump and the alkalinity balance in the sea.

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Section: Discussionmentioning

confidence: 99%

Osmotrophy of dissolved organic carbon by coccolithophores in darkness

2021

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“…Furthermore, high chlorophyll a content in autotrophy has been reported to facilitate high SFA production 51 . A decrease in chlorophyll content under mixotrophy and heterotrophy causes plastid breakage that affects membrane lipid formation 52 . Such a reorganization of cell membrane structure causes recycling of phospholipids, which are mainly composed of PUFA 53 .…”

Section: Distribution Of Fatty Acids and Alteration In Lipid Compositionmentioning

confidence: 99%

Insights into the physiology of Chlorella vulgaris cultivated in sweet sorghum bagasse hydrolysate for sustainable algal biomass and lipid production

Arora

Philippidis

2021

Sci Rep

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Supplementing cultivation media with exogenous carbon sources enhances biomass and lipid production in microalgae. Utilization of renewable organic carbon from agricultural residues can potentially reduce the cost of algae cultivation, while enhancing sustainability. In the present investigation a medium was developed from sweet sorghum bagasse for cultivation of Chlorella under mixotrophic conditions. Using response surface methodology, the optimal values of critical process parameters were determined, namely inoculum cell density (O.D.750) of 0.786, SSB hydrolysate content of the medium 25% v/v, and zero medium salinity, to achieve maximum lipid productivity of 120 mg/L/d. Enhanced biomass (3.44 g/L) and lipid content (40% of dry cell weight) were observed when the alga was cultivated in SSB hydrolysate under mixotrophic conditions compared to heterotrophic and photoautotrophic conditions. A time course investigation revealed distinct physiological responses in terms of cellular growth and biochemical composition of C. vulgaris cultivated in the various trophic modes. The determined carbohydrate and lipid profiles indicate that sugar addition to the cultivation medium boosts neutral lipid synthesis compared to structural lipids, suggesting that carbon flux is channeled towards triacylglycerol synthesis in the cells. Furthermore, the fatty acid profile of lipids extracted from mixotrophically grown cultures contained more saturated and monosaturated fatty acids, which are suitable for biofuel manufacturing. Scale-up studies in a photobioreactor using SSB hydrolysate achieved a biomass concentration of 2.83 g/L consisting of 34% lipids and 26% carbohydrates. These results confirmed that SSB hydrolysate is a promising feedstock for mixotrophic cultivation of Chlorella and synthesis of algal bioproducts and biofuels.

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“…The composition of the SWW adhered to the recommendations of OECD (2001) for synthetic municipal wastewaters with some modi cations as follows: (mg/L) tryptone (160); yeast extract (110), urea (30), NH 4 Cl (40), K 2 HPO 4 (28), NaCl (7), CaCl 2 (4), MgSO 4 (2), Na 2 CO 3 (150) and glucose (100). At 4-day intervals, chemical oxygen demand (COD); ammonium nitrogen (NH 4 -N), phosphate phosphorous (PO…”

Section: Treatment Of Synthetic Municipal Wastewater Using the Microa...mentioning

confidence: 99%

Assessing the wastewater treatment efficiency and bioproducts accumulation potentials of co-cultures of mixotrophic microalgae

Inuwa,

Mahmood,

Irshad

et al. 2024

Preprint

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Utilization of microalgae for simultaneous wastewater treatment and bioproducts accumulation has been a subject of research interest for a while. However, in order to successfully turn this into reality, development of strategies to maximize the yield and performance of the deployed microalgae remains paramount. Herein, the performances and yield of different artificial microalgae consortia were evaluated in a synthetic municipal wastewater medium. The results revealed that the performance of the best monoculture (KL10) was more or less comparable to that of the best binary culture (CK). However, the general pattern of the result revealed that regardless of the species diversity in all the cultures, the average reduction of chemical oxygen demand and total phosphorus, as well as the accumulation of protein, chlorophyll a, chlorophyll b and carotenoids content remined comparable (p > 0.05). Furthermore, the average PO43--P reduction efficiency and biomass productivity of the monocultures was higher than (p < 0.05) those of the co-cultures. Conversely, average NH4-N reduction efficiency and lipid productivity of the binary cultures were superior (p < 0.05) to those of the other culture combinations. Finally, the fatty acids composition, which revealed similar fatty acids profile in all the cultures, suggests the suitability of all the cultures for utilization as feedstock for biodiesel production. In conclusion, the findings of the current study suggest that contrary to the popular notion, raising species diversity in microalgae cultures does not always guarantee increased yield and performance.

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Tailoring of the biochemical profiles of microalgae by employing mixotrophic cultivation

Cited by 20 publications

References 37 publications

Osmotrophy of dissolved organic carbon by coccolithophores in darkness

Osmotrophy of dissolved organic carbon by coccolithophores in darkness

Insights into the physiology of Chlorella vulgaris cultivated in sweet sorghum bagasse hydrolysate for sustainable algal biomass and lipid production

Assessing the wastewater treatment efficiency and bioproducts accumulation potentials of co-cultures of mixotrophic microalgae

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