Tailoring mechanical properties of decellularized extracellular matrix bioink by vitamin B2-induced photo-crosslinking

Jang, Jinah; Kim, Taek Gyoung; Kim, Byoung Soo; Kim, Seok-Won; Kwon, Sang‐Mo; Cho, Dong‐Woo

doi:10.1016/j.actbio.2016.01.013

Cited by 282 publications

(239 citation statements)

References 30 publications

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“…These include 2,20-Azobis[2-methyl-N-(2-hydroxyethyl) propionamide] (VA086), vitaminB2 27 and lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP). Whilst photo cross-linking with very high cell viability has indeed been achieved using VA086 28 , this again required very low-intensity exposure to 365 nm UV-A at 4 mW/cm 2 (again, unacceptably long exposure time requirements for our purposes).…”

Section: Discussionmentioning

confidence: 99%

Handheld Co-Axial Bioprinting: Application to in situ surgical cartilage repair

Duchi

Onofrillo

O’Connell

et al. 2017

Sci Rep

168

147

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Three-dimensional (3D) bioprinting is driving major innovations in the area of cartilage tissue engineering. Extrusion-based 3D bioprinting necessitates a phase change from a liquid bioink to a semi-solid crosslinked network achieved by a photo-initiated free radical polymerization reaction that is known to be cytotoxic. Therefore, the choice of the photocuring conditions has to be carefully addressed to generate a structure stiff enough to withstand the forces phisiologically applied on articular cartilage, while ensuring adequate cell survival for functional chondral repair. We recently developed a handheld 3D printer called “Biopen”. To progress towards translating this freeform biofabrication tool into clinical practice, we aimed to define the ideal bioprinting conditions that would deliver a scaffold with high cell viability and structural stiffness relevant for chondral repair. To fulfill those criteria, free radical cytotoxicity was confined by a co-axial Core/Shell separation. This system allowed the generation of Core/Shell GelMa/HAMa bioscaffolds with stiffness of 200KPa, achieved after only 10 seconds of exposure to 700 mW/cm2 of 365 nm UV-A, containing >90% viable stem cells that retained proliferative capacity. Overall, the Core/Shell handheld 3D bioprinting strategy enabled rapid generation of high modulus bioscaffolds with high cell viability, with potential for in situ surgical cartilage engineering.

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Section: Discussionmentioning

confidence: 99%

Handheld Co-Axial Bioprinting: Application to in situ surgical cartilage repair

Duchi

Onofrillo

O’Connell

et al. 2017

Sci Rep

168

147

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“…Following this study, the same group showed that the dECM bioink can be pre-gelled using vitamin B2-induced covalent cross-linking (Jang et al, 2016a,b,c). Using this approach, a 3D printed cardiac patch composed of multiple-cell lines including human cardiac progenitor cells and mesenchymal stem cells was developed (Jang et al, 2016a,b,c). Although dECM bioinks provide novel opportunities to fabricate tissue specific constructs, the decellularization process requires multiple steps including precise quantification of the DNA and the ECM components, making it a costly approach.…”

Section: Currently Available Bioinksmentioning

confidence: 96%

“…3D printing has a strong potential to become a common fabrication technique in medicine as it enables fabrication of modular and patient-specific scaffolds and devices, and tissue models, with high structural complexity and design flexibility (Murphy and Atala, 2014; Jang et al, 2016a,b,c; Kang et al, 2016; Kuo et al, 2016; Zhang et al, 2016). There is a significant interest in designing novel bioink formulations toward the goal of achieving the “ideal” bioink for each bioprinting technology (Hölzl et al, 2016).…”

Section: Summary and Future Perspectivesmentioning

confidence: 99%

Recent Advances in Bioink Design for 3D Bioprinting of Tissues and Organs

Guvendiren

2017

Front. Bioeng. Biotechnol.

359

258

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There is a growing demand for alternative fabrication approaches to develop tissues and organs as conventional techniques are not capable of fabricating constructs with required structural, mechanical, and biological complexity. 3D bioprinting offers great potential to fabricate highly complex constructs with precise control of structure, mechanics, and biological matter [i.e., cells and extracellular matrix (ECM) components]. 3D bioprinting is an additive manufacturing approach that utilizes a “bioink” to fabricate devices and scaffolds in a layer-by-layer manner. 3D bioprinting allows printing of a cell suspension into a tissue construct with or without a scaffold support. The most common bioinks are cell-laden hydrogels, decellulerized ECM-based solutions, and cell suspensions. In this mini review, a brief description and comparison of the bioprinting methods, including extrusion-based, droplet-based, and laser-based bioprinting, with particular focus on bioink design requirements are presented. We also present the current state of the art in bioink design including the challenges and future directions.

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“…In this versatile technology, cell-laden polymeric solutions [28,154], decellularized ECM components [73], cell suspensions [68], microcarriers [162] or tissue spheroids [185] are loaded into standard disposable syringes, and printed onto a building platform driven by pneumatic (pressurized air), mechanical (piston or screw) or solenoid (electrical pulses)-based dispensing systems [76,111,125,137]. An important advantage of extrusion bioprinting is the ability to print highly viscous polymer solutions containing a wide range of cell densities (up 10 7 cells mL -1 ) [92,182].…”

Section: Extrusion Bioprintingmentioning

confidence: 99%

Advances in bioprinted cell-laden hydrogels for skin tissue engineering

et al. 2017

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Bioprinting technologies are powerful additive biofabrication techniques to produce cellular constructs for skin tissue engineering owing to their unique ability to precisely pattern living and non-living materials in predefined spatial locations. This unique feature, combined with the computer controlled printing and medical imaging techniques, enable researchers and clinicians to generate patient specific constructs partly replicating the intricate compositional and architectural organization of the skin. Bioprinting has been used to automatically dispense hydrogels with skin cells located in prescribed sites that promote skin formation in vitro and in vivo. Current skin bioprinting approaches mostly rely on the sequential printing of fibroblasts and keratinocytes embedded within a homogeneous hydrogel. Although such approaches have already been translated to pre-clinical scenarios, they still present limitations in terms of fully replicating the cellular and extracellular matrix (ECM) heterogeneity in native skin. The success of bioprinting for skin repair strongly depends on the design of printable bioinks capable of supporting the function of printed cells and stimulating the production of new ECM components. To better mimic the human skin, novel developments in dedicated bioprinting technologies, in the design of bioinks, as well as in the printing of vascularised constructs are necessary. This paper presents an overview regarding the use of bioprinting for skin tissue engineering applications. The operating principles of bioprinting technologies are outlined along with requirements of printed skin constructs. Finally, preclinical results are summarized and future perspectives for the field are highlighted.

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Tailoring mechanical properties of decellularized extracellular matrix bioink by vitamin B2-induced photo-crosslinking

Cited by 282 publications

References 30 publications

Handheld Co-Axial Bioprinting: Application to in situ surgical cartilage repair

Handheld Co-Axial Bioprinting: Application to in situ surgical cartilage repair

Recent Advances in Bioink Design for 3D Bioprinting of Tissues and Organs

Advances in bioprinted cell-laden hydrogels for skin tissue engineering

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