Tailoring combinatorial lipid nanoparticles for intracellular delivery of nucleic acids, proteins, and drugs

Li, Yamin; Ye, Zhongfeng; Yang, Hanyi; Xu, Qiaobing

doi:10.1016/j.apsb.2022.04.013

Cited by 38 publications

(27 citation statements)

References 134 publications

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“…Currently, delivery strategies of CRISPR-Cas9 have been composed of two broad categories: viral and nonviral vectors. Since carcinogenesis, insertional mutagenesis, and immunogenicity of viral vectors limits its clinical application, nonviral vectors are growing an alternative strategy 22 , 23 , 24 , 25 , 26 , 27 , 28 . Several nanomaterials-based platforms have been developed to encapsulate Cas9/single-guide RNA (sgRNA) complexes into nanoparticles for delivery of CRISPR, including polymeric nanoparticles, metal–organic frameworks (MOFs), cationic liposomes, gold nanoparticles, and other nanomaterials 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 .…”

Section: Introductionmentioning

confidence: 99%

Tumor-microenvironment activated duplex genome-editing nanoprodrug for sensitized near-infrared titania phototherapy

Pan

et al. 2022

Acta Pharmaceutica Sinica B

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Section: Introductionmentioning

confidence: 99%

Tumor-microenvironment activated duplex genome-editing nanoprodrug for sensitized near-infrared titania phototherapy

Pan

et al. 2022

Acta Pharmaceutica Sinica B

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“…Tu et al found that both miR-10a and miR-10b can inhibit the expression of key factors in the brain-derived neurotrophic factor ( BDNF ) and the TGF-β signaling pathways, thereby inhibiting the follicular granule cell proliferation and induction of granulosa cell apoptosis [ 40 ]. Wei et al found that miR-10b could target BDNF and inhibit the activity of goat ovarian granulosa cells by inhibiting the expression of BDNF [ 41 ]. However, due to the limited research on the functions of miRNAs in the hypothalamus, the deep functions of the miRNAs screened in this study need to be further studied.…”

Section: Discussionmentioning

confidence: 99%

Integrated Transcriptome Analysis Reveals the Crucial mRNAs and miRNAs Related to Fecundity in the Hypothalamus of Yunshang Black Goats during the Luteal Phase

Han

Liang

Liu

et al. 2022

Animals

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A normal estrus cycle is essential for the breeding of goats, and the luteal phase accounts for most of the estrus cycle. The corpus luteum (CL) formed during the luteal phase is a transient endocrine gland that is crucial for the reproductive cycle and pregnancy maintenance, and is controlled by many regulatory factors. However, the molecular mechanism of the hypothalamus effect on the reproductive performance of different litter sizes during the luteal phase of goats has not been elucidated. In this study, RNA-sequencing was used to analyze the mRNA and miRNA expression profiles of the hypothalamic tissues with the high-fecundity goats during the luteal phase (LP-HF) and low-fecundity goats during the luteal phase (LP-LF). The RNA-seq results found that there were 1963 differentially expressed genes (DEGs) (890 up-regulated and 1073 down-regulated). The miRNA-seq identified 57 differentially expressed miRNAs (DEMs), including 11 up-regulated and 46 down-regulated, of which 199 DEGs were predicted to be potential target genes of DEMs. Meanwhile, the functional enrichment analysis identified several mRNA-miRNA pairs involved in the regulation of the hypothalamic activity, such as the common target gene MEA1 of novel-miR-972, novel-miR-125 and novel-miR-403, which can play a certain role as a related gene of the reproductive development in the hypothalamic–pituitary–gonadal (HPG) axis and its regulated network, by regulating the androgen secretion. While another target gene ADIPOR2 of the novel-miR-403, is distributed in the hypothalamus and affects the reproductive system through a central role on the HPG axis and a peripheral role in the gonadal tissue. An annotation analysis of the DE miRNA-mRNA pairs identified targets related to biological processes, such as anion binding (GO:0043168) and small molecule binding (GO: 0036094). Subsequently, the KEGG(Kyoto Encyclopedia of Genes and Genomes) pathways were performed to analyze the miRNA-mRNA pairs with negatively correlated miRNAs. We found that the GnRH signaling pathway (ko04912), the estrogen signaling pathway (ko04915), the Fc gamma R-mediated phagocytosis (ko04666), and the IL-17 signaling pathway (ko04657), etc., were directly and indirectly associated with the reproductive process. These targeting interactions may be closely related to the reproductive performance of goats. The results of this study provide a reference for further research on the molecular regulation mechanism for the high fertility in goats.

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“…Additionally, as artemisnin and medicinal plants have been widely applied in clinic for years, their safety, biocomparability and in vivo dynamics are guaranteed. All the traits make ADNVs a promising therapy or drug carriers for treating tumors or other related illness [56,57].…”

Section: Discussionmentioning

confidence: 99%

Medicinal plant-derived mtDNA via nanovesicles induces the cGAS-STING pathway to remold tumor-associated macrophages for tumor regression

Liu

Xiang

Jin

et al. 2022

Preprint

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Plant-derived nanovesicles (PDNVs) have been proposed as a major mechanism for inter-kingdom interaction and communication, but the effector components enclosed and the mechanisms involved are largely unknown. The plant Artemisia annua, known for its anti-malaria properties, exhibits a wide range of biological activities including the immunoregulatory and anti-tumor properties with the mechanisms to be further addressed. Here, we isolated and purified the exosome-like particles from Artemisia annua, and characterized them as nano-scaled and membrane-bound, which were therefore termed artemisia-derived nanovesicles (ADNVs). Remarkably, the vesicles displayed a potential to inhibit tumor growth and boost anti-tumor immunity, primarily through remolding tumor microenvironment and reprogramming tumor-associated macrophages (TAMs). More importantly, we identified plant-derived mitochondrial DNA (mtDNA), upon internalized into TAMs via the vesicles, as a major effector mechanism to induce the cGAS-STING pathway driving the shift of pro-tumor macrophages to anti-tumor phenotype. Furthermore, our data showed that administration of ADNVs greatly improved the efficacy of PD-L1 inhibitor, a prototypic immune checkpoint inhibitor (ICI), in a murine lung cancer model. Together, the present study, for the first time, to our knowledge, unravels an inter-kingdom interaction wherein medical plant-derived mtDNA, via the nanovesicles, induces the immunostimulatory signaling in mammal immune cells for resetting anti-tumor immunity.

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Tailoring combinatorial lipid nanoparticles for intracellular delivery of nucleic acids, proteins, and drugs

Cited by 38 publications

References 134 publications

Tumor-microenvironment activated duplex genome-editing nanoprodrug for sensitized near-infrared titania phototherapy

Tumor-microenvironment activated duplex genome-editing nanoprodrug for sensitized near-infrared titania phototherapy

Integrated Transcriptome Analysis Reveals the Crucial mRNAs and miRNAs Related to Fecundity in the Hypothalamus of Yunshang Black Goats during the Luteal Phase

Medicinal plant-derived mtDNA via nanovesicles induces the cGAS-STING pathway to remold tumor-associated macrophages for tumor regression

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