Tailoring Catalytic Activity of Pt Nanoparticles Encapsulated Inside Dendrimers by Tuning Nanoparticle Sizes with Subnanometer Accuracy for Sensitive Chemiluminescence-Based Analyses

Lim, Hyojung; Ju, Youngwon; Kim, Joohoon

doi:10.1021/acs.analchem.6b00073

Cited by 54 publications

(38 citation statements)

References 45 publications

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“…Peroxidases have been found to catalyze the oxidation of various compounds including Amplex Red in the presence of molecular oxygen although the catalyzed oxidation is more dramatic with the addition of hydrogen peroxide (Klapper and Hackett, 1963; Wang et al, 2017). The Pt-DENs have been proved to show a peroxidase-like catalytic activity (Ju and Kim, 2015; Lim et al, 2016), where the nanoscale size of this molecule enabled us to readily mix with the culture medium and introduce into the microchannel. More importantly, since the Pt-DENs are considered to be inert with epithelium without any cytotoxicity, we tested it in our experiments to estimate the in situ oxygen level with the Amplex Red.…”

Section: Discussionmentioning

confidence: 99%

A Robust Longitudinal Co-culture of Obligate Anaerobic Gut Microbiome With Human Intestinal Epithelium in an Anoxic-Oxic Interface-on-a-Chip

Shin

Massidda

et al. 2019

Front. Bioeng. Biotechnol.

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136

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The majority of human gut microbiome is comprised of obligate anaerobic bacteria that exert essential metabolic functions in the human colon. These anaerobic gut bacteria constantly crosstalk with the colonic epithelium in a mucosal anoxic-oxic interface (AOI). However, in vitro recreation of the metabolically mismatched colonic AOI has been technically challenging. Furthermore, stable co-culture of the obligate anaerobic commensal microbiome and epithelial cells in a mechanically dynamic condition is essential for demonstrating the host-gut microbiome crosstalk. Here, we developed an anoxic-oxic interface-on-a-chip (AOI Chip) by leveraging a modified human gut-on-a-chip to demonstrate a controlled oxygen gradient in the lumen-capillary transepithelial interface by flowing anoxic and oxic culture medium at various physiological milieus. Computational simulation and experimental results revealed that the presence of the epithelial cell layer and the flow-dependent conditioning in the lumen microchannel is necessary and sufficient to create the steady-state vertical oxygen gradient in the AOI Chip. We confirmed that the created AOI does not compromise the viability, barrier function, mucin production, and the expression and localization of tight junction proteins in the 3D intestinal epithelial layer. Two obligate anaerobic commensal gut microbiome, Bifidobacterium adolescentis and Eubacterium hallii, that exert metabolic cross-feeding in vivo, were independently co-cultured with epithelial cells in the AOI Chip for up to a week without compromising any cell viability. Our new protocol for creating an AOI in a microfluidic gut-on-a-chip may enable to demonstrate the key physiological interactions of obligate anaerobic gut microbiome with the host cells associated with intestinal metabolism, homeostasis, and immune regulation.

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Section: Discussionmentioning

confidence: 99%

A Robust Longitudinal Co-culture of Obligate Anaerobic Gut Microbiome With Human Intestinal Epithelium in an Anoxic-Oxic Interface-on-a-Chip

Shin

Massidda

et al. 2019

Front. Bioeng. Biotechnol.

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136

134

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“…Electrochemiluminescence (ECL) is light emission from excited state luminophores generated by electrochemical reactions, which possesses better spatiotemporal control in comparison with chemiluminescence (Richter, 2004;Hu and Xu, 2010;Hesari and Ding, 2017;Liu et al, 2019). Because no external light illumination is required to generate ECL emission, ECL has unique advantages, such as low background and high sensitivity, et al It has manifested itself to be a powerful transduction signal for the determination of small biomolecules, DNA and protein biomarkers in biosensing and clinical diagnostics (Doeven et al, 2014;Wu et al, 2014;Lim et al, 2016;Bist et al, 2017;Ji et al, 2017;Yang et al, 2018;Zhou et al, 2018;Zhang et al, 2019a;Jones et al, 2020). Moreover, ECL has recently emerged as a useful surface analysis technology because of its intrinsic surface-confined and surface-sensitive nature (Guo et al, 2017;Zhang et al, 2019b;Zanut et al, 2019;Ma et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Confined Electrochemiluminescence Generation at Ultra-High-Density Gold Microwell Electrodes

et al. 2021

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Electrochemiluminescence (ECL) imaging analysis based on the ultra-high-density microwell electrode array (UMEA) has been successfully used in biosensing and diagnostics, while the studies of ECL generation mechanisms with spatial resolution remain scarce. Herein we fabricate a gold-coated polydimethylsiloxane (PDMS) UMEA using electroless deposition method for the visualization of ECL reaction process at the single microwell level in conjunction with using microscopic ECL imaging technique, demonstrating that the microwell gold walls are indeed capable of enhancing the ECL generation. For the classical ECL system involving tris(2,2′-bipyridyl)ruthenium (Ru(bpy)32+) and tri-n-propylamine (TPrA), the ECL image of a single microwell appears as a surface-confined ring, indicating the ECL intensity generated inside the well is much stronger than that on the top surface of UMEA. Moreover, at a low concentration of Ru(bpy)32+, the ECL image remains to be ring-shaped with the increase of exposure time, because of the limited lifetime of TPrA radical cations TPrA+•. In combination with the theoretical simulation, the ring-shaped ECL image is resolved to originate from the superposition effect of the mass diffusion fields at both microwell wall and bottom surfaces.

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“…Pt NPs have high catalytic activities for many chemical reactions, which made it an effective catalyst for CL . And Pt NPs frequently display unusual catalytic properties, depending upon their size, shape and stabilizing agents . However, the detection of Hg 2+ using Pt NPs combined with FI‐CL has not been reported yet.…”

Section: Introductionmentioning

confidence: 99%

Pt NPs catalyzed chemiluminescence method for Hg²⁺ detection based on a flow injection system

Zheng

Zhao

et al. 2019

Electrophoresis

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Establishing a simple and accurate method for Hg2+ detection is of great importance for the environment and human health. In this work, platinum nanoparticles (Pt NPs) with different capped agents and morphologies were synthesized. It was found that Pt NPs exhibited peroxidase‐like activity that can catalyze the chemiluminescence (CL) of the luminol system without H2O2. The most intensive CL signals were obtained by using PVP‐capped Pt NPs as catalysis. Based on the fact that Hg2+ could further enhance the CL intensity in the Pt NPs‐luminol CL system, a Pt NPs‐catalyzed CL method based on a flow injection system is developed for the sensitive analysis of Hg2+. When the concentration of Hg2+ in the system increases, the CL intensity would together increase, thereby achieving sensitive Hg2+ detection. The limit of detection (LOD) was calculated to be 8.6 nM. This developed method provides a simple and rapid approach for the sensitive detection of Hg2+ and shows great promise for applications in other complex systems.

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Tailoring Catalytic Activity of Pt Nanoparticles Encapsulated Inside Dendrimers by Tuning Nanoparticle Sizes with Subnanometer Accuracy for Sensitive Chemiluminescence-Based Analyses

Cited by 54 publications

References 45 publications

A Robust Longitudinal Co-culture of Obligate Anaerobic Gut Microbiome With Human Intestinal Epithelium in an Anoxic-Oxic Interface-on-a-Chip

A Robust Longitudinal Co-culture of Obligate Anaerobic Gut Microbiome With Human Intestinal Epithelium in an Anoxic-Oxic Interface-on-a-Chip

Confined Electrochemiluminescence Generation at Ultra-High-Density Gold Microwell Electrodes

Pt NPs catalyzed chemiluminescence method for Hg²⁺ detection based on a flow injection system

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Tailoring Catalytic Activity of Pt Nanoparticles Encapsulated Inside Dendrimers by Tuning Nanoparticle Sizes with Subnanometer Accuracy for Sensitive Chemiluminescence-Based Analyses

Cited by 54 publications

References 45 publications

A Robust Longitudinal Co-culture of Obligate Anaerobic Gut Microbiome With Human Intestinal Epithelium in an Anoxic-Oxic Interface-on-a-Chip

A Robust Longitudinal Co-culture of Obligate Anaerobic Gut Microbiome With Human Intestinal Epithelium in an Anoxic-Oxic Interface-on-a-Chip

Confined Electrochemiluminescence Generation at Ultra-High-Density Gold Microwell Electrodes

Pt NPs catalyzed chemiluminescence method for Hg2+ detection based on a flow injection system

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Product

Resources

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Pt NPs catalyzed chemiluminescence method for Hg²⁺ detection based on a flow injection system