Tailored upconversion nanomaterial: A hybrid nano fluorescent sensor for evaluating efficacy of lactate dehydrogenase inhibitors as anticancer drugs

Wang, Zhuo; Qiu, Xueer; Xi, Wen-Song; Tang, Ming Lee; Liu, Jinliang; Jiang, Hong; Sun, Lining

doi:10.1016/j.snb.2021.130417

Cited by 18 publications

(15 citation statements)

References 62 publications

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“…To achieve better pattern recognition, ImageJ software (version 1.53n 7, Wayne Rasband, NIH Retires, Bethesda, MD, USA) was used to process the digital signal of fluorescence images, including the color level, intensity, and corresponding area [ 44 , 45 ]. Due to the fluorescence of the green color staining by secondary antibodies, all images were filtered with green color for the digital level of its intensity.…”

Section: Methodsmentioning

confidence: 99%

The Utilization of Tunable Transducer Elements Formed by the Manipulation of Magnetic Beads with Different Sizes via Optically Induced Dielectrophoresis (ODEP) for High Signal-to-Noise Ratios (SNRs) and Multiplex Fluorescence-Based Biosensing Applications

Yang

Chu

et al. 2022

Biosensors

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Magnetic beads improve biosensing performance by means of their small volume and controllability by magnetic force. In this study, a new technique composed of optically induced dielectrodphoresis (ODEP) manipulation and image processing was used to enhance the signal-to-noise ratio of the fluorescence for stained magnetic beads. According to natural advantages of size-dependent particle isolation by ODEP manipulation, biomarkers in clinical samples can be easily separated by different sizes of magnetic beads with corresponding captured antibodies, and rapidly distinguished by separated location of immunofluorescence. To verify the feasibility of the concept, magnetic beads with three different diameters, including 21.8, 8.7, and 4.2 μm, were easily separated and collected into specific patterns in the defined target zone treated as three dynamic transducer elements to evaluate fluorescence results. In magnetic beads with diameter of 4.2 μm, the lowest signal-to-noise ratio between stained and nonstained magnetic beads was 3.5. With the help of ODEP accumulation and detection threshold setting of 32, the signal-to-noise ratio was increased to 77.4, which makes this method more reliable. With the further optimization of specific antibodies immobilized on different-size magnetic beads in the future, this platform can be a potential candidate for a high-efficiency sensor array in clinical applications.

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Section: Methodsmentioning

confidence: 99%

The Utilization of Tunable Transducer Elements Formed by the Manipulation of Magnetic Beads with Different Sizes via Optically Induced Dielectrophoresis (ODEP) for High Signal-to-Noise Ratios (SNRs) and Multiplex Fluorescence-Based Biosensing Applications

Yang

Chu

et al. 2022

Biosensors

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“…[5,6] Förster resonance energy transfer (FRET) assays have many advantages for biosensing and clinical diagnostics because they are sensitive, easy to perform, and do not require time-consuming separation or washing steps. [7][8][9][10] Energy transfer with UCNPs has been widely used for biosensing and bioimaging, [11][12][13][14][15][16][17] including the detection of deoxyribonucleic acid (DNA). [18][19][20] However, UCNPs are usually quite large (>20 nm in diameter) and their surfaces must be coated to prevent UCL quenching in aqueous media, which further increases their sizes.…”

Section: Introductionmentioning

confidence: 99%

DNA‐Coated Upconversion Nanoparticles for Sensitive Nucleic Acid FRET Biosensing

Francés‐Soriano

Estébanez

Pérez‐Prieto

et al. 2022

Adv Funct Materials

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Despite the significant advancements of developing upconversion nanoparticles (UCNPs) for high performance biosensing and bioimaging, the development of DNA‐functionalized UCNPs with thin coatings, efficient surface passivation, and fully functional DNAs for hybridization sensing in biological media remains extremely challenging. Here, a straightforward concept of labeling DNA to a thin polysulfonate polymer layer on UCNPs is presented. Both UCNPs and DNA preserve their full functionality as demonstrated by Förster resonance energy transfer (FRET) hybridization assays with a Cy3‐conjugated complementary DNA. The important bioanalytical benefits of the UCNP‐DNA nanohybrids are demonstrated by their implementation into rapid and wash‐free microRNA hybridization FRET assays. Using a simple fiber‐coupled USB‐spectrometer and 980 nm diode laser excitation in a 96‐well microplate, ratiometric FRET from UCNP‐to‐Cy3 can quantify miR20a in a 0.01–10 × 10−9 m concentration range with a detection limit of 30 × 10−12 m (4.5 fmol of miR20a), which is more than 20‐fold lower compared to quantum dot‐based FRET assays. The functional thin‐coating DNA‐UCNP nanohybrids have a strong potential for translating UCNPs into advanced DNA‐based biosensing, bioimaging, and high‐throughput and point‐of‐care clinical diagnostics.

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“…In the development of sensors or probe systems, rare earth hybrid materials are widely used due to their sensitivity and excellent fluorescent properties [ 27 , 28 ]. In situ sensing in complex biological environments requires higher stability, better sensitivity, narrow emission, long fluorescence lifetime, and good biocompatibility [ 29 , 30 ].…”

Section: Introductionmentioning

confidence: 99%

Lanthanide (Eu3+/Tb3+)-Loaded γ-Cyclodextrin Nano-Aggregates for Smart Sensing of the Anticancer Drug Irinotecan

Guo

Liu

Tang

et al. 2022

IJMS

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The clinical use of anticancer drugs necessitates new technologies for their safe, sensitive, and selective detection. In this article, lanthanide (Eu3+ and Tb3+)-loaded γ-cyclodextrin nano-aggregates (ECA and TCA) are reported, which sensitively detects the anticancer drug irinotecan by fluorescence intensity changes. Fluorescent lanthanide (Eu3+ and Tb3+) complexes exhibit high fluorescence intensity, narrow and distinct emission bands, long fluorescence lifetime, and insensitivity to photobleaching. However, these lanthanide (Eu3+ and Tb3+) complexes are essentially hydrophobic, toxic, and non-biocompatible. Lanthanide (Eu3+ and Tb3+) complexes were loaded into naturally hydrophilic γ-cyclodextrin to form fluorescent nano-aggregates. The biological nontoxicity and cytocompatibility of ECA and TCA fluorescent nanoparticles were demonstrated by cytotoxicity experiments. The ECA and TCA fluorescence nanosensors can detect irinotecan selectively and sensitively through the change of fluorescence intensity, with detection limits of 6.80 μM and 2.89 μM, respectively. ECA can safely detect irinotecan in the cellular environment, while TCA can detect irinotecan intracellularly and is suitable for cell labeling.

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Tailored upconversion nanomaterial: A hybrid nano fluorescent sensor for evaluating efficacy of lactate dehydrogenase inhibitors as anticancer drugs

Cited by 18 publications

References 62 publications

The Utilization of Tunable Transducer Elements Formed by the Manipulation of Magnetic Beads with Different Sizes via Optically Induced Dielectrophoresis (ODEP) for High Signal-to-Noise Ratios (SNRs) and Multiplex Fluorescence-Based Biosensing Applications

The Utilization of Tunable Transducer Elements Formed by the Manipulation of Magnetic Beads with Different Sizes via Optically Induced Dielectrophoresis (ODEP) for High Signal-to-Noise Ratios (SNRs) and Multiplex Fluorescence-Based Biosensing Applications

DNA‐Coated Upconversion Nanoparticles for Sensitive Nucleic Acid FRET Biosensing

Lanthanide (Eu3+/Tb3+)-Loaded γ-Cyclodextrin Nano-Aggregates for Smart Sensing of the Anticancer Drug Irinotecan

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