Tailored synbiotic powder (functional food) to prevent hyperphosphataemia (kidney disorder)

Abstract

Hyperphosphataemia is treated with phosphate binders, which can cause adverse effects. Spray-dried synbiotic powder (SP) composed of Lactobacillus casei JCM1134 (a phosphate-accumulating organism; PAO) and Aloe vera is potentially a safer alternative for efficient phosphate removal. In this study, a novel strategy was developed; lysine-derivatized deacetylated A. vera (DAVK) was synthesised and fabricated on phosphate-deficient PAO (PDP) for efficient phosphate transfer and then spray-dried with the supernatant of DAV centrifugation to form a sacrificial layer on PDP for SP integrity during gastric passage. In vitro experiments revealed that PAO removed only 1.6% of the phosphate from synthetic media, whereas SP removed 89%, 87%, and 67% (w/v) of the phosphate from milk, soft drink, and synthetic media, respectively, confirming the protective role of A. vera and efficient phosphate transport. Compared with commercial binders, SP effectively removed phosphate from synthetic media, whereas SP and CaCO3 exhibited comparative results for milk and soft drink. Importantly, CaCO3 caused hypercalcaemia. Thus, the described SP presents a promising tool to prevent hyperphosphataemia. This study also revealed a novel factor: diets of patients with chronic kidney disease should be monitored to determine the optimal phosphate binders, as phosphate removal performance depends on the accessible phosphate forms.

Understudied Hyperphosphatemia (Chronic Kidney Disease) Treatment Targets and New Biological Approaches

“…Moreover, the synbiotic formulation demonstrated efficient phosphate removal relative to the calcium carbonate from milk and soft drinks. Overall, the synbiotic formulation stands out as the first preference for phosphate removal from phosphate-rich synthetic media (15.7 mg dipotassium monohydrogen phosphate salt/mL) under simulated in vitro conditions [ 71 ].…”

Long Short‐Term Memory‐Based Multiomics Reveal Lactobacillus casei‐Derived Postbiotics Inhibiting Lipids Digestion via Mediating the Upregulation of α‐Helices in Lipase

“…[5] Both in vitro cultures and in vivo experiments illustrated that the excellent phosphate-enrichment capacity of L. casei JCM 1134 in the digestive tract allowed it to present a crucial role in metabolic diseases mediated by phosphate imbalance. [6,7] Notwithstanding, concerns about the possible adverse effects of live probiotic cells in the health therapy of high-risk patients, including bacteremia and pneumonia have driven continued interest in clinical trials using alternative approaches. Increasing evidence has indicated that the primary health-elevating properties of probiotics maybe connected with the nonviable byproducts known as postbiotics.…”

“…[ 5 ] Both in vitro cultures and in vivo experiments illustrated that the excellent phosphate‐enrichment capacity of L. casei JCM 1134 in the digestive tract allowed it to present a crucial role in metabolic diseases mediated by phosphate imbalance. [ 6,7 ]…”