Tailored Multivalent Targeting of Siglecs with Photosensitizing Liposome Nanocarriers

Abstract

The modification of surfaces with multiple ligands allows the formation of platforms for the study of multivalency in diverse processes. Herein we use this approach for the implementation of a photosensitizer (PS)-nanocarrier system that binds efficiently to siglec-10, a member of the CD33 family of siglecs (sialic acid (SA)-binding immunoglobulin-like lectins). In particular, a zinc phthalocyanine derivative bearing three SA moieties (PcSA) has been incorporated in the membrane of small unilamellar vesicles (SUVs), retaining its photophysical properties upon insertion into the SUV's membrane. The interaction of these biohybrid systems with human siglec-10-displaying supported lipid bilayers (SLBs) has shown the occurrence of weakly multivalent, superselective interactions between vesicle and SLB. The SLB therefore acts as an excellent cell membrane mimic, while the binding with PS-loaded SUVs shows the potential for targeting siglec-expressing cells with photosensitizing nanocarriers.

Quantifying Siglec-sialylated ligand interactions: a versatile 19F-T2 CPMG filtered competitive NMR displacement assay

“…57,58 Thus, as next step, a multivalent ligand based on a phthalocyanine (Pc) decorated with 12 sialic acid moieties ( ZnPc Neu5Ac) 52 was tested. Sialylated dendritic Pc ligands like ZnPc Neu5Ac and related derivatives are also relevant due to their optical properties, 59 which make them potential photosensitizers for photodynamic therapy (PDT) of cancer and other diseases where Siglecs are involved. Fittingly, the addition of the multivalent ZnPc Neu5Ac molecule induced a signicant displacement of the diF a2,6SLN spy molecule for both Siglec-9 and Siglec-15 (Fig.…”

Recent Advances in Kaolinite Nanoclay as Drug Carrier for Bioapplications: A Review

“…Organic nanocarriers, including liposomes, [ 23 ] dendrimers, [ 24 ] polymeric nanocarriers, [ 25 ] micelles, [ 26 ] and viral nanocarriers, are versatile, less toxic, and capable of binding a variety of pharmaceuticals as well as drug delivery ligands. [ 27 , 28 , 29 ] Among these, liposomes were previously studied as drug carriers, and a lot of research experience and clinical trial data have been accumulated; many related products have been marketed, such as Vyxeos, [ 30 ] a liposomal formulation of erythromycin/alglucoside for the treatment of leukemia, and Arikayce, [ 31 ] a liposomal suspension of amikacin sulfate for the treatment of lung diseases.…”

Distinct Binding Properties of Neutravidin and Streptavidin Proteins to Biotinylated Supported Lipid Bilayers: Implications for Sensor Functionalization

“…One emerging use of avidin–biotin technology is in the field of supported lipid bilayer (SLB) coatings [ 12 ], which are antifouling cell membrane mimics that are formed through the self-assembly of phospholipid molecules on solid surfaces and are widely used in biosensor development [ 13 , 14 , 15 , 16 ]. Generally, biotinylated lipids are incorporated in the SLB that acts as a platform upon which avidin or related protein analogues are specifically attached via biotin coupling, and such platforms can be used in various biosensing applications ranging from fundamental biophysical studies to molecular diagnostics and cancer cell detection [ 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 ] ( Figure 1 A).…”

“…Sensors 2022, 22, x FOR PEER REVIEW 2 of 15 solid surfaces and are widely used in biosensor development [13][14][15][16]. Generally, biotinylated lipids are incorporated in the SLB that acts as a platform upon which avidin or related protein analogues are specifically attached via biotin coupling, and such platforms can be used in various biosensing applications ranging from fundamental biophysical studies to molecular diagnostics and cancer cell detection [17][18][19][20][21][22][23][24][25][26][27][28] (Figure 1A). Avidin is an egg-white glycoprotein that has four binding sites for biotin along with a high isoelectric point (pI) around pH 10.5 (ref.…”