Tailored Melt Electrowritten Scaffolds for the Generation of Sheet‐Like Tissue Constructs from Multicellular Spheroids

McMaster, Rebecca; Hoefner, Christiane; Hrynevich, Andrei; Blum, Carina; Wiesner, Miriam; Wittmann, Katharina; Dargaville, Tim R.; Bauer‐Kreisel, Petra; Groll, Jürgen; Dalton, Paul D.; Blunk, Torsten

doi:10.1002/adhm.201801326

Cited by 50 publications

(48 citation statements)

References 38 publications

(40 reference statements)

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“…Poly-ɛ-caprolactone (PCL) has become the most popular scaffold material due to its nontoxicity, low melting point, good mechanical properties, and high biocompatibility 24,25 . Many PCL-based scaffolds have been developed via MEW for tissue engineering applications [26][27][28][29][30][31][32][33][34][35][36][37] : organized microfiber networks contribute to reinforcing hydrogels for chondrocyte differentiation 30 ; scaffolds with box-structured pores are adapted for the simultaneous seeding of multicellular spheroids 31,32 ; stretchable hexagonal microstructures can enhance the beating rate and cardiac maturation-related marker expression as a potential heart patch 33 ; and multiple layers of fibroblasts can be assembled with the aid of MEW scaffolds as a human prostate cancer microtissue model 34 . Many researchers in tissue engineering have paid continuous attention to PCL scaffolds made by MEW.…”

Section: Introductionmentioning

confidence: 99%

A hierarchically ordered compacted coil scaffold for tissue regeneration

Zhang

Wan

et al. 2020

NPG Asia Mater

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Hierarchically ordered scaffold has a great impact on cell patterning and tissue engineering. The introduction of controllable coils into a scaffold offers an additional unique structural feature compared to conventional linear patterned scaffolds and can greatly increase interior complexity and versatility. In this work, 3D coil compacted scaffolds with hierarchically ordered patterns and tunable coil densities created using speed-programmed melt electrospinning writing (sMEW) successfully led to in vitro cell growth in patterns with tunable cell density. Subcutaneous implantation in mice showed great in vivo biocompatibility, as evidenced by no significant increase in tumor necrosis factor α (TNF-α) and interleukin 6 (IL-6) levels in mouse serum. In addition, a lumbar vertebra was successfully printed for mesenchymal stem cells to grow in the desired pattern. A long-range patterned matrix composed of programmable short-range compacted coils enabled the design of complex structures, e.g., for tailored implants, by readily depositing short-range coil-compacted secondary architectures along with customized primary design.

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Section: Introductionmentioning

confidence: 99%

A hierarchically ordered compacted coil scaffold for tissue regeneration

Zhang

Wan

et al. 2020

NPG Asia Mater

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“…The fusion capability of multicellular spheroids representing such intermediate tissue modules has been characterized in detail and utilized in proof‐of‐principle studies for tissue generation . The bioprinting of such spheroids or microtissues into either 3D plotted or MEW‐generated thermoplastic polymer scaffolds has recently been proposed and demonstrated, facilitating the assembly into larger tissue units …”

Section: Strategies To Evolve From Shape To Functionmentioning

confidence: 99%

From Shape to Function: The Next Step in Bioprinting

et al. 2020

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In 2013, the “biofabrication window” was introduced to reflect the processing challenge for the fields of biofabrication and bioprinting. At that time, the lack of printable materials that could serve as cell‐laden bioinks, as well as the limitations of printing and assembly methods, presented a major constraint. However, recent developments have now resulted in the availability of a plethora of bioinks, new printing approaches, and the technological advancement of established techniques. Nevertheless, it remains largely unknown which materials and technical parameters are essential for the fabrication of intrinsically hierarchical cell–material constructs that truly mimic biologically functional tissue. In order to achieve this, it is urged that the field now shift its focus from materials and technologies toward the biological development of the resulting constructs. Therefore, herein, the recent material and technological advances since the introduction of the biofabrication window are briefly summarized, i.e., approaches how to generate shape, to then focus the discussion on how to acquire the biological function within this context. In particular, a vision of how biological function can evolve from the possibility to determine shape is outlined.

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“…Cell spheroids seeded in the scaffolds porosities attached and formed aggregates due to the low fiber diameter of the gradient scaffold and proper interconnectivity. In another study by McMaster and colleagues [97], cell spheroids from adipose-derived stromal cells were seeded on a mesh-like structure. Some ultra-fine threading was printed at the bottom of the scaffold by manipulating the processing parameters in order to hold the cell spheroids.…”

Section: Opportunities For Mew In Tissue Engineeringmentioning

confidence: 99%

Biomimicry in Bio-Manufacturing: Developments in Melt Electrospinning Writing Technology Towards Hybrid Biomanufacturing

et al. 2019

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Melt electrospinning writing has been emerged as a promising technique in the field of tissue engineering, with the capability of fabricating controllable and highly ordered complex three-dimensional geometries from a wide range of polymers. This three-dimensional (3D) printing method can be used to fabricate scaffolds biomimicking extracellular matrix of replaced tissue with the required mechanical properties. However, controlled and homogeneous cell attachment on melt electrospun fibers is a challenge. The combination of melt electrospinning writing with other tissue engineering approaches, called hybrid biomanufacturing, has introduced new perspectives and increased its potential applications in tissue engineering. In this review, principles and key parameters, challenges, and opportunities of melt electrospinning writing, and particularly, recent approaches and materials in this field are introduced. Subsequently, hybrid biomanufacturing strategies are presented for improved biological and mechanical properties of the manufactured porous structures. An overview of the possible hybrid setups and applications, future perspective of hybrid processes, guidelines, and opportunities in different areas of tissue/organ engineering are also highlighted.

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Tailored Melt Electrowritten Scaffolds for the Generation of Sheet‐Like Tissue Constructs from Multicellular Spheroids

Abstract: COMMUNICATION 1801326 (1 of 7)

Cited by 50 publications

References 38 publications

A hierarchically ordered compacted coil scaffold for tissue regeneration

A hierarchically ordered compacted coil scaffold for tissue regeneration

From Shape to Function: The Next Step in Bioprinting

Biomimicry in Bio-Manufacturing: Developments in Melt Electrospinning Writing Technology Towards Hybrid Biomanufacturing

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