Tailor-made novel electrospun polycaprolactone/polyethyleneimine fiber membranes for laccase immobilization: An all-in-one material to biodegrade textile dyes and phenolic compounds

Metal ion‐mediated immobilization of catalase on poly(ε‐caprolactone)/polyethyleneimine electrospun nanofibers

“…The difference in the intensities of the significant peaks of PCL and PCL/PEI nanofiber shows that the incorporation of PEI causes the increment of crystallinity of composite nanofibers. The lack of substantial differences in the XRD patterns of nanofibers indicates that their structures were not altered by the immobilization of catalase on the nanofibers 92 …”

“…The lack of substantial differences in the XRD patterns of nanofibers indicates that their structures were not altered by the immobilization of catalase on the nanofibers. 92 3.2 | Effect of pH on PCL/PEI-Cu 2+ -CAT Effect of pH on the activity of free and immobilized catalase is depicted in Figure 7. The highest activity values of both free and immobilized enzymes were obtained at pH 7.0, but there were no significant sharp decreases in more acidic and alkali pH regions.…”

Propelling of Enzyme Activity by Using Different Triggering Strategies: Applications and Perspectives

“…Conceptually, an immobilized enzyme can be defined as an enzyme that is physically confined or localized to a particular solid support, whose catalytic activities are maintained, and can be used repeatedly and continuously. , In scientific research, many methods of enzyme immobilization have been reported, including adsorption, covalent bonding, cross-linking, and entrapment; each has advantages and disadvantages. ,, Owing to the improved stability, reusability, and high enzyme retention of the proposed supports, more and more types of materials have been used as supports to immobilize various enzymes . To date, many carriers have been used for immobilization including natural − or synthetic polymers, copolymers, hydrogels, beads, − blends, nanofibers, − nanoparticles, − composites, − metal–organic frameworks, , etc. We refer readers to several recent review articles that comprehensively review these areas (method and support). ,− Although the scientific literature is full of reports on new carrier supports and new approaches to immobilize enzymes, most of them are not even suitable for enzyme immobilization as the immobilization method and carrier used are insufficient to improve the enzyme properties of the matrix.…”