Tailor-made Janus lectin with dual avidity assembles glycoconjugate multilayers and crosslinks protocells

Ribeiro, João-Paulo; Villringer, Sarah; Goyard, David; Coche-Guérente, Liliane; Höferlin, Manuela; Renaudet, Olivier; Römer, Winfried; Imberty, Anne

doi:10.1039/c8sc02730g

Cited by 29 publications

(48 citation statements)

References 29 publications

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“…The title compound was prepared according to already published protocol. Analytical data were in agreement with the literature (Ribeiro et al, 2018).…”

Section: Methodssupporting

confidence: 90%

Heteroglycoclusters With Dual Nanomolar Affinities for the Lectins LecA and LecB From Pseudomonas aeruginosa

et al. 2019

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Multivalent structures displaying different instead of similar sugar units, namely heteroglycoclusters (hGCs), are stimulating the efforts of glycochemists for developing compounds with new biological properties. Here we report a four-step strategy to synthesize hexadecavalent hGCs displaying eight copies of αFuc and βGal. These compounds were tested for the binding to lectins LecA and LecB from Pseudomonas aeruginosa. While parent fucosylated (19) and galactosylated (20) homoclusters present nanomolar affinity with LecB and LecA, respectively, we observed that hGCs combining these sugars (11 and 13) maintain their binding potency with both lectins despite the presence of an unspecific sugar. The added multivalency is therefore not a barrier for efficient recognition by bacterial receptors and it opens the route for adding different sugars that can be selected for their immunomodulatory properties.

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“…The title compound was prepared according to already published protocol. Analytical data were in agreement with the literature (Ribeiro et al, 2018).…”

Section: Methodssupporting

confidence: 90%

Heteroglycoclusters With Dual Nanomolar Affinities for the Lectins LecA and LecB From Pseudomonas aeruginosa

et al. 2019

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“…An interesting example of how artificial lectins can be created has been reported by Ribeiro et al 99 They engineered the first chimeric, bispecific lectin, with two rationally oriented and distinct recognition surfaces, which are able to bind both fucosylated and sialylated glycoconjugates (Fig. 5).…”

Section: Lectinsmentioning

confidence: 99%

The challenges of glycan recognition with natural and artificial receptors

et al. 2019

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“…In another study, Ribeiro et al engineered the first bispecific lectin (termed Janus lectin) with two distinct recognition surfaces for fucosylated and sialylated glycoconjugates, respectively. This bispecific lectin could bridge protocells carrying two different types of glycoconjugates and form a network of heterogeneous protocells [140]. royalsocietypublishing.org/journal/rsfs Interface Focus 9: 20180084…”

Section: Assembly Of Prototissues By Protocell Cross-linking and Adhementioning

confidence: 99%

Glycan-decorated protocells: novel features for rebuilding cellular processes

Omidvar

Römer

2019

Interface Focus.

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In synthetic biology approaches, lipid vesicles are widely used as protocell models. While many compounds have been encapsulated in vesicles (e.g. DNA, cytoskeleton and enzymes), the incorporation of glycocalyx components in the lipid bilayer has attracted much less attention so far. In recent years, glycoconjugates have been integrated in the membrane of giant unilamellar vesicles (GUVs). These minimal membrane systems have largely contributed to shed light on the molecular mechanisms of cellular processes. In this review, we first introduce several preparation and biophysical characterization methods of GUVs. Then, we highlight specific applications of protocells investigating glycolipid-mediated endocytosis of toxins, viruses and bacteria. In addition, we delineate how prototissues have been assembled from glycan-decorated protocells by using lectin-mediated cross-linking of opposed glycoreceptors (e.g. glycolipids and glycopeptides). In future applications, glycan-decorated protocells might be useful for investigating cell–cell interactions (e.g. adhesion and communication). We also speculate about the implication of lectin–glycoreceptor interactions in membrane fusion processes.

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Tailor-made Janus lectin with dual avidity assembles glycoconjugate multilayers and crosslinks protocells

Abstract: The double-faced Janus lectin, designed by assembling sialic acid and fucose-specific lectin, organize multivalent heteroglyco compounds in mulitlayered material, and glycosylated protocells in prototissues.

Cited by 29 publications

References 29 publications

Heteroglycoclusters With Dual Nanomolar Affinities for the Lectins LecA and LecB From Pseudomonas aeruginosa

Heteroglycoclusters With Dual Nanomolar Affinities for the Lectins LecA and LecB From Pseudomonas aeruginosa

The challenges of glycan recognition with natural and artificial receptors

Glycan-decorated protocells: novel features for rebuilding cellular processes

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