Tailor-made active enzyme aggregates in vivo for efficient degradation of biomass polysaccharide into reducing sugar

Ge, Zhongqi; Liu, Guanzhang; Zeng, Bo; Liu, Xin; Zhang, Guangya

doi:10.1007/s13399-022-03578-8

Cited by 2 publications

(3 citation statements)

References 49 publications

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“…Considering the simple purification process, high purity, and low cost of DvCA8.0-F, it will have great potentials for large-scale preparation. By staining on the micron-scale DvCA8.0-F with crystal violet, rod-shaped and ellipsoidal morphologies (Figure S4d) with sizes of about 1–2 μm were detected under an optical microscope, which was similar to some previous studies. ,− The formation of micron-sized supramolecules may be attributed to the complex internal and external forces, such as the hydrophobic or hydrophilic interactions, as well as electrostatic interactions between DvCA8.0 and ferritin subunits. , Additionally, high protein concentration in the cells also increases the risk of protein–protein interactions and aggregation …”

Section: Resultssupporting

confidence: 83%

“…As shown in Figure S4a, the ingeniously designed DvCA8.0-F could be effectively expressed and form into supramolecules in E. coli . Upon being released from the cells, DvCA8.0-F could undergo spontaneous precipitation at the bottom of the tube.…”

Section: Resultsmentioning

confidence: 99%

“…S4a, the ingeniously designed DvCA8.0-F could be effectively expressed and form into supramolecules in E. coli. 61 Upon being released from the cells, DvCA8.0-F could undergo spontaneous precipitation at the bottom of the tube. To achieve efficient purification of DvCA8.0-F, centrifugal force was carefully investigated, and the results are shown in Figure 1a.…”

Section: Optimization Of Co 2 Absorption and Desorption Conditionsmentioning

confidence: 99%

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Cost-Effective Preparation of Carbonic Anhydrase with Superior Performance for Assisting Amine and Amino Acid Ionic Liquid Blends in CO₂ Absorption and Desorption

Mao,

Chen,

Zhang

et al. 2024

ACS Sustainable Chem. Eng.

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The utilization of carbonic anhydrases (CAs) for CO 2 capture aligns with the principles of environmental sustainability. However, there is an urgent need to minimize the cost of CA purification while improving their performance in harsh environments. A novel strategy was proposed to solve the above problems by employing the ferritin-tagged CA variant (DvCA8.0-F). The purification is achieved by low-speed centrifugation, yielding 92% activity recovery and 95% purity. After 14 weeks of incubation in a 50% MDEA solution at 50 °C, DvCA8.0-F maintained activity nearly equivalent to the initial activity, which showed remarkable stability compared to the free ones. Encouragingly, DvCA8.0-F had a yield of up to 800 mg/L, allowing for cost-effective CA-assisted CO 2 absorption and desorption in MDEA solutions. The results show that DvCA8.0-F reduced the absorption time from 70 to 50 min at 40 °C and desorption time from 40 to 25 min at 96 °C in 25%MDEA+1%[N1111][Gly] solution with a final CO 2 load and a stripped amount of CO 2 at the same time. DvCA8.0-F exhibits the maximum reaction rate at 96 °C; the CO 2 regeneration efficiency improved from 60% at 87 °C to 72%. When the concentration of DvCA8.0-F was 1.5 g/L, the absorption rate of CO 2 reached about 90% of that of the 25% MEA solution. These results demonstrate the simplicity of preparation and the outstanding properties of DvCA8.0-F, which paves a feasible way for CA-assisted CO 2 capture. This exciting strategy may also have excellent potential in enzymes for innovative chemical synthesis to achieve the sustainable development of green chemistry.

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Section: Resultssupporting

confidence: 83%

Section: Resultsmentioning

confidence: 99%

Section: Optimization Of Co 2 Absorption and Desorption Conditionsmentioning

confidence: 99%

See 1 more Smart Citation

Cost-Effective Preparation of Carbonic Anhydrase with Superior Performance for Assisting Amine and Amino Acid Ionic Liquid Blends in CO₂ Absorption and Desorption

Mao,

Chen,

Zhang

et al. 2024

ACS Sustainable Chem. Eng.

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An antifouling epoxy coated metal surface containing silica-immobilized carbonic anhydrase supraparticles for CO2 capture through microalgae

Liu,

Li,

Yuan

et al. 2024

International Journal of Biological Macromolecules

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Tailor-made active enzyme aggregates in vivo for efficient degradation of biomass polysaccharide into reducing sugar

Cited by 2 publications

References 49 publications

Cost-Effective Preparation of Carbonic Anhydrase with Superior Performance for Assisting Amine and Amino Acid Ionic Liquid Blends in CO₂ Absorption and Desorption

Cost-Effective Preparation of Carbonic Anhydrase with Superior Performance for Assisting Amine and Amino Acid Ionic Liquid Blends in CO₂ Absorption and Desorption

An antifouling epoxy coated metal surface containing silica-immobilized carbonic anhydrase supraparticles for CO2 capture through microalgae

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Tailor-made active enzyme aggregates in vivo for efficient degradation of biomass polysaccharide into reducing sugar

Cited by 2 publications

References 49 publications

Cost-Effective Preparation of Carbonic Anhydrase with Superior Performance for Assisting Amine and Amino Acid Ionic Liquid Blends in CO2 Absorption and Desorption

Cost-Effective Preparation of Carbonic Anhydrase with Superior Performance for Assisting Amine and Amino Acid Ionic Liquid Blends in CO2 Absorption and Desorption

An antifouling epoxy coated metal surface containing silica-immobilized carbonic anhydrase supraparticles for CO2 capture through microalgae

Contact Info

Product

Resources

About

Cost-Effective Preparation of Carbonic Anhydrase with Superior Performance for Assisting Amine and Amino Acid Ionic Liquid Blends in CO₂ Absorption and Desorption

Cost-Effective Preparation of Carbonic Anhydrase with Superior Performance for Assisting Amine and Amino Acid Ionic Liquid Blends in CO₂ Absorption and Desorption