Taguchi approach for anti-heat stress prescription compatibility in mice spleen lymphocytes In Vitro

Abstract

Heat stress (HS) may induce immunosuppression as well as inhibit the proliferation of lymphocytes. This study evaluated the effects on immune function of our prescription on splenic lymphocytes under HS as well as its compatibility. The effects of four herbal extracts from Agastache rugosa, Atractylodes lancea, Cortex Phellodendri, and Gypsum Fibrosum on heat treated splenic lymphocytes were investigated and the compatibility of the prescription was also explored by using the Taguchi method. This study revealed changes in proliferation by traditional Chinese medicines of splenic lymphocytes after HS. Proliferation in the HS group was significantly lower than the control group. Under HS, the effects of higher concentrations of Agastache rugosa (100 and 200 μg/mL), Atractylodes lancea (100 and 200 μg/mL), Cortex Phellodendri (50 and 100 μg/mL) and Gypsum Fibrosum (100 and 200 μg/mL) caused a significant increase on ConA/LPS-induced proliferation of lymphocytes than lower concentrations. We, therefore, conclude that the prescription of traditional Chinese medicines may recover splenic lymphocytes from the immunosuppression induced by HS. The Taguchi design, which allows rapid and high efficiency for the selection of the best conditions for our prescription on HS-treated splenic lymphocytes, demonstrated that Agastache rugosa (200 μg/mL), Atractylodes lancea (200 μg/mL), Cortex Phellodendri (100 μg/mL) and Gypsum Fibrosum (100 μg/mL) were the optimal conditions for the prescription. The validation experiment confirmed that our composition in optimum extraction conditions enhanced effects on ConA or LPS-stimulated lymphocytes under HS. The results showed that the Taguchi optimization approach is a suitable method for optimization of the composition of prescription.

Effects of Huang Bai (Phellodendri Cortex) and Three Other Herbs on GnRH and GH Levels in GT1–7 and GH3 Cells

“…Previous studies have demonstrated that the bark of Huang Bai has antidiabetic [ 18 ], anti-heat stress [ 19 ], anti-inflammatory [ 20 ], antimicrobial [ 21 , 22 ], and neuroprotective [ 23 ] effects and that the spike of Xia Ku Cao exhibits antitumor [ 24 , 25 ] and immunosuppressive [ 26 ] activities. Similarly, the rhizome of Zhi Mu possesses antidiabetic, anti-inflammatory, anticoagulatory, antihypertensive, antitumor, antioxidative, antimicrobial, antiviral, antiosteoporosis, anti-skin aging, and neuroprotective [ 27 ] effects; additionally, the fruit of Mai Ya exerts antihyperprolactinemia activity [ 28 ].…”

Traditional Chinese Medicine Prescriptions Enhance Growth Performance of Heat Stressed Beef Cattle by Relieving Heat Stress Responses and Increasing Apparent Nutrient Digestibility

“…It was also reported that the effects of these TCM decoctions in vitro enhanced the antioxidant status and reduced lipid peroxidation in heat-stressed intestinal crypt-like epithelial cells (Guo et al, 2011). As well, the effects of these herbal extracts may recover splenic lymphocytes from the immunosuppression induced by heat stress (Zhu et al, 2011). …”

Therapeutic potential of traditional Chinese medicine on heat stroke

“…According to veterinary research, Cangzhu possessed the ability of anti-heat stress in spleen and intestine ( Xu et al, 2017 ). In addition, the polysaccharide of Cangzhu was shown to have the protective effects on endoplasmic reticulum stress and apoptosis in HS-induced spleen injury ( Zhu et al, 2011 ). Chanxinlian ( Andrographis paniculata ) is a type of herb with a strong bitter taste used to treat sore throats, common cold, and noninfective diarrhea ( Dai et al, 2019 ).…”