Tagma-specific distribution of FXPRLamides in the nervous system of the American cockroach

Predel, Reinhard; Eckert, Manfred

doi:10.1002/(sici)1096-9861(20000410)419:3<352::aid-cne7>3.0.co;2-0

Cited by 48 publications

(19 citation statements)

References 39 publications

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“…The location of the PK/PBAN-like peptides in mosquitoes is similar to other insects in general (Sato et al 1998;Choi et al 2001;Choi et al 2011;Predel and Eckert, 2000). The SEG contains three groups of immunoreactive neurons that correspond to the presumptive mandibular, maxillary, and labial neuromeres.…”

Section: Discussionmentioning

confidence: 73%

“…A similar two gene expression pattern for PK/PBAN-like peptides has been proposed for Solenopsis sp. (Choi et al 2009), Manduca sexta (Loi and Tublitz 2004), Bombyx mori (Roller et al 2008), and Periplaneta americana (Predel and Eckert 2000;Schoofs et al 1992). A search of the Anopholes gambiae genome indicates that these same two genes putatively code for peptides in the PK/PBAN family (Riehle et al 2002) The PKs produced by capa and hugin have been confirmed by mass spectroscopy in the mosquito A. aegypti (Predel et al 2010).…”

Section: Introductionmentioning

confidence: 94%

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Pyrokinin/PBAN-like peptides in the central nervous system of mosquitoes

2014

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The pyrokinin/pheromone biosynthesis activating neuropeptide (PBAN) family of peptides is characterized by a common C-terminal pentapeptide, FXPRLamide, which is required for diverse physiological functions in various insects. Polyclonal antisera against the C-terminus was utilized to determine the location of cell bodies and axons in the central nervous systems of larval and adult mosquitoes. Immunoreactive material was detected in three groups of neurons in the subesophageal ganglion of larvae and adults. The corpora cardiaca of both larvae and adults contained immunoreactivity indicating potential release into circulation. The adult and larval brains had at least one pair of immunoreactive neurons in the protocerebrum with the adult brain having additional immunoreactive neurons in the dorsal medial part of the protocerebrum. The ventral ganglia of both larvae and adults each contained one pair of neurons that sent their axons to a perisympathetic organ associated with each abdominal ganglion. These results indicate that the mosquito nervous system contains pyrokinin/PBAN-like peptides and that these peptides could be released into the hemolymph. The peptides in insects and mosquitoes are produced by two genes, capa and pk/pban. Utilizing PCR protocols, we demonstrate that products of the capa gene could be produced in the abdominal ventral ganglia and the products of the pk/pban gene could be produced in the subesophageal ganglion. Two receptors for pyrokinin peptides were differentially localized to various tissues. Abstract Pyrokinin/pheromone biosynthesis activating neuropeptide (PBAN) family of peptides is characterized by a common C-terminal pentapeptide, FXPRLamide, that is required for diverse physiological functions in various insects. Polyclonal antisera against the C-terminus was utilized to determine the location of cell bodies and axons in the central nervous systems of larval and adult mosquitoes. Immunoreactive material was detected in three groups of neurons in the subesophageal ganglion of larvae and adults. The corpora cardiaca of both larvae and adults contained immunoreactivity indicating potential release into circulation. The adult and larval brains had at least one pair of immunoreactive neurons in the protocerebrum with the adult brain having additional immunoreactive neurons in the dorsal medial part of the protocerebrum. The ventral ganglia of both larvae and adults each contained one pair of neurons that sent their axons to a perisympathetic organ associated with each abdominal ganglion. These results indicate that the mosquito nervous system contains pyrokinin/PBAN-like peptides and that these peptides could be released into the hemolymph. The peptides in insects and mosquitoes are produced by two genes, capa and pk/pban. Utilizing PCR protocols we demonstrate that products of the capa gene could be produced in the abdominal ventral ganglia and the products of the pk/pban gene could be produced in the subesophageal ganglion. Two receptors for pyrokinin peptides were differ...

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Section: Discussionmentioning

confidence: 73%

Section: Introductionmentioning

confidence: 94%

Pyrokinin/PBAN-like peptides in the central nervous system of mosquitoes

2014

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“…It is most closely related to the B. mori diapause hormone, sharing the COOH-terminal LWFGPRL-amide with this peptide (Fig. 1C), and pyrokinins 5 and 6 from the American cockroach Periplaneta americana (24,25). Diapause hormone is responsible for inducing embryonic diapause, which does not occur in Drosophila, and the function of cockroach pyrokinins 5 and 6 is un-known.…”

Section: Resultsmentioning

confidence: 99%

“…i: Putative members of the CAP2b family, including capa-1 and capa-2, cleavable deduced peptides encoded by the capa gene (this study); CAP2b from Manduca sexta (3); and LYMNAEA_SCPB and LYMNAEA_SCPA, i.e., Lymnaea stagnalis small cardioactive peptides (13,23). ii: Putative members of the pheromone biosynthesis-activating neuropeptide (PBAN) family and some related peptides, including PBAN_BOMMO from Bombyx mori (39); capa-3, cleavable deduced peptide encoded by the Drosophila melanogaster capa gene (this study); pyrokinins 5 and 6 from the American cockroach Periplaneta americana (24,25); DH (Bombyx), i.e., Bombyx mori diapause hormone (29); hugin (short), cleavable deduced peptide encoded by the Drosophila hugin gene (GenBank accession no. CAB88005); and ETH1 and ETH2, Drosophila ecdysistriggering hormones 1 and 2 (ETH1 and ETH2) (22).…”

Section: Resultsmentioning

confidence: 99%

Two nitridergic peptides are encoded by the genecapabilityinDrosophila melanogaster

Kean

Cazenave

Costes

et al. 2002

American Journal of Physiology-Regulatory, Integrative and Comp

201

240

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A Drosophila gene ( capability, capa) at 99D on chromosome 3R potentially encodes three neuropeptides: GANMGLYAFPRV-amide (capa-1), ASGLVAFPRV-amide (capa-2), and TGPSASSGLWGPRL-amide (capa-3). Capa-1 and capa-2 are related to the lepidopteran hormone cardioacceleratory peptide 2b, while capa-3 is a novel member of the pheromone biosynthesis-activating neuropeptide/diapause hormone/pyrokinin family. By immunocytochemistry, we identified four pairs of neuroendocrine cells likely to release the capa peptides into the hemolymph: one pair in the subesophageal ganglion and the other three in the abdominal neuromeres. In the Malpighian (renal) tubule, capa-1 and capa-2 increase fluid secretion rates, stimulate nitric oxide production, and elevate intracellular Ca2+ and cGMP in principal cells. Capa-stimulated fluid secretion, but not intracellular Ca2+ concentration rise, is inhibited by the guanylate cyclase inhibitor methylene blue. The actions of capa-1 and capa-2 are not synergistic, implying that both act on the same pathways in tubules. The capa gene is thus the first to be shown to encode neuropeptides that act on renal fluid production through nitric oxide.

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“…dSDDYGHMRF-NH 2 [15] 1369.6 corazonin dTFQYSRGWTN-NH 2 [20] 1443.6 unsulfated Pea-SK EQFDDYGHMRF-NH 2 [15] 1447.7 Pea-K-4 DHLPHDV YSPRL-NH 2 [17] 1590.8 Pea-PK-6 SESEVPG MWFGPRL-NH 2 [21] 1857.9 ? ?…”

Section: Resultsmentioning

confidence: 99%

Matrix-assisted laser desorption/ionization quadrupole Time-of-Flight Mass Spectrometry: An elegant tool for peptidomics

Verhaert¹,

Uttenweiler-Joseph

Vries³

et al. 2001

Proteomics

110

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A Matrix-assisted laser desorption/ionization hybrid quadrupole orthogonal acceleration time-of-flight mass spectrometer was employed to acquire neuropeptide mass spectra, directly from neuropeptide secreting tissue deposited on the sample target, in the presence of dihydroxybenzoic acid as matrix. The cockroach corpus cardiacum served as model neuroendocrine tissue. Twelve neuropeptide ion peaks, with mass-to-charge ratio values ranging between 800 and 3,000 Da were selected for tandem mass spectrometry. All peptides below 1,600 Da could be fully sequenced; tandem mass spectrometry analysis of the remaining (three) largest peptides resulted in (limited) sequence tags, which, also due to unavailability of an appropriate neuropeptide structure database, did not allow complete structure elucidation.

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Tagma-specific distribution of FXPRLamides in the nervous system of the American cockroach

Cited by 48 publications

References 39 publications

Pyrokinin/PBAN-like peptides in the central nervous system of mosquitoes

Pyrokinin/PBAN-like peptides in the central nervous system of mosquitoes

Two nitridergic peptides are encoded by the genecapabilityinDrosophila melanogaster

Matrix-assisted laser desorption/ionization quadrupole Time-of-Flight Mass Spectrometry: An elegant tool for peptidomics

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