Tagging of T cells with superparamagnetic iron oxide: Uptake kinetics and relaxometry

Bulte, Jeff W.M.; Laughlin, Peter G.; Jordan, Elaine; Vu, Tran Anh; Vymazal, Josef; Frank, Joseph A.

doi:10.1016/s1076-6332(96)80564-2

Cited by 49 publications

(34 citation statements)

References 6 publications

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“…In general, most nonphagocytic cells do not take up MNPs efficiently or require cells to be exposed to high amounts of iron in culture [21,22,[34][35][36]. Most magnetic nanoparticles require transfection agents for adequate internalization by stem cells, although it has been reported that Feridex complexed with PL blocked the differentiation of human MSCs into chondrocytes [37].…”

Section: Discussionmentioning

confidence: 99%

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Optimization of the magnetic labeling of human neural stem cells and MRI visualization in the hemiparkinsonian rat brain

Ramos-Gómez¹,

Seiz²,

Martínez‐Serrano³

2016

Nano Online

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Background: Magnetic resonance imaging is the ideal modality for non-invasive in vivo cell tracking allowing for longitudinal studies over time. Cells labeled with superparamagnetic iron oxide nanoparticles have been shown to induce sufficient contrast for in vivo magnetic resonance imaging enabling the in vivo analysis of the final location of the transplanted cells. For magnetic nanoparticles to be useful, a high internalization efficiency of the particles is required without compromising cell function, as well as validation of the magnetic nanoparticles behaviour inside the cells. Results: In this work, we report the development, optimization and validation of an efficient procedure to label human neural stem cells with commercial nanoparticles in the absence of transfection agents. Magnetic nanoparticles used here do not affect cell viability, cell morphology, cell differentiation or cell cycle dynamics. Moreover, human neural stem cells progeny labeled with magnetic nanoparticles are easily and non-invasively detected long time after transplantation in a rat model of Parkinson's disease (up to 5 months post-grafting) by magnetic resonance imaging. Conclusions: These findings support the use of commercial MNPs to track cells for short-and mid-term periods after transplantation for studies of brain cell replacement therapy. Nevertheless, long-term MR images should be interpreted with caution due to the possibility that some MNPs may be expelled from the transplanted cells and internalized by host microglial cells.

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Section: Discussionmentioning

confidence: 99%

“…Consequently, these contrast agents are not used as isolated reagents to label hNSCs or other mammalian cells [20][21][22]. In most cases, internalization of nanoparticles by hNSCs requires the use of transfection agents (TAs), like protamine sulfate (PS) or poly-L-lysine (PL) to achieve an efficient labeling of the stem cells.…”

Section: Introductionmentioning

confidence: 99%

Optimization of the magnetic labeling of human neural stem cells and MRI visualization in the hemiparkinsonian rat brain

Ramos-Gómez¹,

Seiz²,

Martínez‐Serrano³

2016

Nano Online

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“…using lectins [143], by conjugating antigen-specific monoclonal antibodies (MION-46 L [144]), or by binding specific peptide chains (HIV-1 Tat peptide) to the dextran coating of SPIO (MION-Tat, CLIO-Tat [145]). Moreover, the use of transfection media [146], polyamines, lipids, or dendrimers [147] can result in enhanced cellular SPIO incorporation.…”

Section: Cell Labeling and Cell Imaging In Mrimentioning

confidence: 99%

Superparamagnetic Iron Oxide Nanoparticles in Biomedicine: Applications and Developments in Diagnostics and Therapy

Ittrich¹,

Peldschus²,

Raabe³

et al. 2013

Fortschr Röntgenstr

122

101

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Superparamagnetic iron oxide nanoparticles (SPIO) can be used to image physiological processes and anatomical, cellular and molecular changes in diseases. The clinical applications range from the imaging of tumors and metastases in the liver, spleen and bone marrow, the imaging of lymph nodes and the CNS, MRA and perfusion imaging to atherosclerotic plaque and thrombosis imaging. New experimental approaches in molecular imaging describe undirected SPIO trapping (passive targeting) in inflammation, tumors and associated macrophages as well as the directed accumulation of SPIO ligands (active targeting) in tumor endothelia and tumor cells, areas of apoptosis, infarction, inflammation and degeneration in cardiovascular and neurological diseases, in atherosclerotic plaques or thrombi. The labeling of stem or immune cells allows the visualization of cell therapies or transplant rejections. The coupling of SPIO to ligands, radio- and/or chemotherapeutics, embedding in carrier systems or activatable smart sensor probes and their externally controlled focusing (physical targeting) enable molecular tumor therapies or the imaging of metabolic and enzymatic processes. Monodisperse SPIO with defined physicochemical and pharmacodynamic properties may improve SPIO-based MRI in the future and as targeted probes in diagnostic magnetic resonance (DMR) using chip-based ?NMR may significantly expand the spectrum of in vitro analysis methods for biomarker, pathogens and tumor cells. Magnetic particle imaging (MPI) as a new imaging modality offers new applications for SPIO in cardiovascular, oncological, cellular and molecular diagnostics and therapy. Key Points: Citation Format:

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“…Different modifications and coating of SPIO particles through linking to lectins [30], HIV-tat peptide [31,32], internalizing monoclonal antibodies [33,34], or dendrimers [35,36] have been described to improve the internalization of the contrast agent, with indubitable advantages in terms of labeling efficiency but also with potential disadvantages in terms of technical complexity, poor availability, species specificity, and biosafety, issues that are critical for the potential clinical translation of these labeling procedures.…”

Section: Introductionmentioning

confidence: 99%

Efficient In Vitro Labeling of Human Neural Precursor Cells with Superparamagnetic Iron Oxide Particles: Relevance for In Vivo Cell Tracking

Neri¹,

Maderna²,

Cavazzin³

et al. 2007

Stem Cells

148

123

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Recent studies have raised appealing possibilities of replacing damaged or lost neural cells by transplanting in vitro-expanded neural precursor cells (NPCs) and/or their progeny. Magnetic resonance (MR) tracking of superparamagnetic iron oxide (SPIO)-labeled cells is a noninvasive technique to track transplanted cells in longitudinal studies on living animals. Murine NPCs and human mesenchymal or hematopoietic stem cells can be efficiently labeled by SPIOs. However, the validation of SPIO-based protocols to label human neural precursor cells (hNPCs) has not been extensively addressed. Here, we report the development and validation of optimized protocols using two SPIOs (Sinerem and Endorem) to label human hNPCs that display bona fide stem cell features in vitro. A careful titration of both SPIOs was required to set the conditions resulting in efficient cell labeling without impairment of cell survival, proliferation, self-renewal, and multipotency. In vivo magnetic resonance imaging (MRI) combined with histology and confocal microscopy indicated that low numbers (5 ؋ 10 3 to 1 ؋ 10 4 ) of viable SPIO-labeled hNPCs could be efficiently detected in the short term after transplantation in the adult murine brain and could be tracked for at least 1 month in longitudinal studies. By using this approach, we also clarified the impact of donor cell death to the MR signal. This study describes a simple protocol to label NPCs of human origin using SPIOs at optimized low dosages and demonstrates the feasibility of noninvasive imaging of labeled cells after transplantation in the brain; it also evidentiates potential limitations of the technique that have to be considered, particularly in the perspective of neural cell-based clinical applications. STEM CELLS 2008;26:505-516 Disclosure of potential conflicts of interest is found at the end of this article.

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Tagging of T cells with superparamagnetic iron oxide: Uptake kinetics and relaxometry

Cited by 49 publications

References 6 publications

Optimization of the magnetic labeling of human neural stem cells and MRI visualization in the hemiparkinsonian rat brain

Optimization of the magnetic labeling of human neural stem cells and MRI visualization in the hemiparkinsonian rat brain

Superparamagnetic Iron Oxide Nanoparticles in Biomedicine: Applications and Developments in Diagnostics and Therapy

Efficient In Vitro Labeling of Human Neural Precursor Cells with Superparamagnetic Iron Oxide Particles: Relevance for In Vivo Cell Tracking

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