Taenia taeniaeformis: Inactivation of metacestodes by gossypol in vitro

Gossypol, a natural polyphenolic compound, induces growth-inhibitory and antiparasitic effects in Taenia taeniaeformis metacestodes in vivo and in vitro. We investigated the uptake and localization of [3H]-gossypol in this parasite. Metacestodes were incubated in 10(-5) M [3H]-gossypol at 37 degrees C. Parasites steadily took up tritium activity over the first 3 h of incubation, after which a plateau was maintained for the duration of the experiment. Tissue: medium radioactivity ratios revealed that intralarval tritium activity matched extralarval activity within 30 min of incubation and continued to increase with time. Reverse-phase high-performance liquid chromatographic (HPLC) analysis confirmed tissue incorporation of tritium activity that manifested as a single radioactive species. Autoradiography localized [3H]-gossypol to the tegument, calcareous corpuscles, and parenchyma over the first 2 h of incubation. By 6 h, parenchymal radioactivity had disappeared. T. taeniaeformis metacestodes rapidly take up and accumulate [3H]-gossypol in vitro. This accumulation is apparently selective for specific sites, which may have implications for gossypol's metacestocidal action.

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In vitro uptake and autoradiographic localization of tritiated gossypol inTaenia taeniaeformis metacestodes

Kulp

Rikihisa

Lin

et al. 1993

Parasitol Res

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Gossypol content on leaves and seeds from some wild <i>Malvaceae</i> species

Sotelo

Villavicencio

Montalvo

et al. 2004

Afr. J. Trad. Compl. Alt. Med.

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Ten Malvaceae species were analysed for their seed and leaf gossypol content by HPLC.The results showed that gossypol is common in most of the Malvaceae family, but its concentration varies among the species and also among the varieties of the same specie. We found that Hampea integerrina Schldt., had two fold more gossypol in its seeds than Gossypium hirsutum L., from which the compound was initially isolated and its antifertility effect studied. The toxic effects of gossypol earlier observed against several parasitic protozoa and viruses makes these findings very important, since the Malvaceae specimens studied here antiseptic. On the other hand, it is also noteworthy that in Hibicus sabdariffa L., flower, traditionally used in refreshing drinks, no gossypol was detected in its seeds or leaves.

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Taenia taeniaeformis: Inactivation of metacestodes by gossypol in vitro

Cited by 2 publications

References 15 publications

In vitro uptake and autoradiographic localization of tritiated gossypol inTaenia taeniaeformis metacestodes

In vitro uptake and autoradiographic localization of tritiated gossypol inTaenia taeniaeformis metacestodes

Gossypol content on leaves and seeds from some wild <i>Malvaceae</i> species

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