Taenia solium: characterization of a small heat shock protein (Tsol-sHSP35.6) and its possible relevance to the diagnosis and pathogenesis of neurocysticercosis

Ferrer, Elizabeth; González, Luís Miguel; MildredFoster-Cuevas,; Cortéz, María Milagros; Dávila, Iris; Rodríguez, Mercedes; Sciutto, Edda; Harrison, L.J.S.; Parkhouse, R. M. E.; Gárate, Teresa

doi:10.1016/j.exppara.2004.11.014

Cited by 35 publications

(24 citation statements)

References 62 publications

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“…Taenia crassiceps-specific IgG1 and IgG2a levels were determined by ELISA as previously described (Ferrer et al 2005). Results are expressed as the maximal sera dilution (endpoint titer) where OD was detected.…”

Section: Nos Inhibitionmentioning

confidence: 99%

Nitric oxide contributes to host resistance against experimental Taenia crassiceps cysticercosis

et al. 2007

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The immune mechanisms that underlie resistance and susceptibility to cysticercosis are not completely understood. In this paper, using susceptible BALB/c mice and resistant STAT6-/-BALB/c mice, we have analyzed the role of nitric oxide (NO) in determining the outcome of murine cysticercosis caused by the cestode Taenia crassiceps. After T. crassiceps infection, wild-type BALB/c mice developed a strong Th2-like response, produced high levels of IgG1, IgE, IL-5, IL-4, and discrete levels of NO, and remained susceptible to T. crassiceps infection. In contrast, similarly infected BALB/c mice treated with N(omega)-nitro-L-arginine methyl ester (L-NAME, an inhibitor of NO synthase) mounted a similar immune response but with lower levels of NO and harbored nearly 100% more parasites than N(omega)-nitro-D-arginine methyl ester (D-NAME, inactive enantiomer)-treated mice. To further analyze the role of NO in murine cysticercosis, we treated STAT6-/-male mice (known to be highly resistant to T. crassiceps) with L-NAME during 8 weeks of infection. As expected, STAT6-/-mice mounted a strong Th1-like response, produced high levels of IgG2a, IFN-gamma, and IL-17, whereas their macrophages displayed increased transcripts of tumor necrosis factor (TNF)-alpha as well as inducible nitric oxide synthase (iNOS) and efficiently controlled T. crassiceps infection. However, STAT6-/-male mice receiving L-NAME mounted a similar immune response but with lower iNOS transcripts concomitantly with decreased levels of NO in sera and displayed significantly higher parasite burdens. These findings suggest that macrophage activation and NO production are effector mechanisms that importantly contribute in host resistance to T. crassiceps infection. The immune mechanisms that underlie resistance and susceptibility to cysticercosis are not completely understood. In this paper, using susceptible BALB/c mice and resistant STAT6-/-BALB/c mice, we have analyzed the role of nitric oxide (NO) in determining the outcome of murine cysticercosis caused by the cestode Taenia crassiceps. After T. crassiceps infection, wild-type BALB/c mice developed a strong Th2-like response, produced high levels of IgG1, IgE, IL-5, IL-4, and discrete levels of NO, and remained susceptible to T. crassiceps infection. In contrast, similarly infected BALB/c mice treated with N(omega)-nitro-L-arginine methyl ester (L-NAME, an inhibitor of NO synthase) mounted a similar immune response but with lower levels of NO and harbored nearly 100% more parasites than N(omega)-nitro-d-arginine methyl ester (D-NAME, inactive enantiomer)-treated mice. To further analyze the role of NO in murine cysticercosis, we treated STAT6-/-male mice (known to be highly resistant to T. crassiceps) with L-NAME during 8 weeks of infection. As expected, STAT6-/-mice mounted a strong Th1-like response, produced high levels of IgG2a, IFN-gamma, and IL-17, whereas their macrophages displayed increased transcripts of tumor necrosis factor (TNF)-alpha as well as inducible nitric oxide synthase (iNOS) and efficien...

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Section: Nos Inhibitionmentioning

confidence: 99%

Nitric oxide contributes to host resistance against experimental Taenia crassiceps cysticercosis

et al. 2007

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“…Isolation of a T. solium metacestode tegument antigen (TEGTsol) by immunoscreening A T. solium metacestode cDNA library was prepared using the Uni-ZAP® XR library kit (Strategene, La Jolla, CA, USA; Montero et al 2003;Ferrer et al 2005b). As T. saginata and T. solium are of such close taxonomic relationship that each can be regarded as a model for the other (Harrison and Parkhouse 1989), the library was screened using a pool of sera from rabbits immunized against metacestode excretory/secretory and glycoprotein antigens from these two taeniids, exactly as described previously (Montero et al 2003;Ferrer et al 2005b).…”

Section: Methodsmentioning

confidence: 99%

“…As T. saginata and T. solium are of such close taxonomic relationship that each can be regarded as a model for the other (Harrison and Parkhouse 1989), the library was screened using a pool of sera from rabbits immunized against metacestode excretory/secretory and glycoprotein antigens from these two taeniids, exactly as described previously (Montero et al 2003;Ferrer et al 2005b). A total of 1.2×10 6 recombinant phages of a T. solium metacestode cDNA library were screened with a rabbit antiserum pool.…”

Section: Methodsmentioning

confidence: 99%

The Taenia saginata homologue of the major surface antigen of Echinococcus spp. is immunogenic and 97% identical to its Taenia solium homologue

et al. 2007

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The TEG-Tsag gene of Taenia saginata is homologous to the genes expressing the two major surface antigens of Echinococcus spp. (EM10 and EG10). Surface antigens of parasites are logical candidates for vaccines, and in this paper we demonstrate that cattle vaccinated with the recombinant TEG-Tsag protein, either used singly or in conjunction with the recombinant HP6-Tsag protein, the major 18 kDa surface/secreted antigen of T. saginata oncospheres, produce excellent antibody responses to both these recombinant proteins. Thus TEG-Tsag may have utility as a vaccine and also as a diagnostic tool for bovine cysticercosis. In addition, as we now demonstrate a 97% homology between TEG-Tsag and its Taenia solium homologue, TEG-Tsol, this latter molecule may have similar potential in the control of human and porcine cysticercosis. The TEG molecule is characterized by an N-terminal FERM domain and a C-terminal ERM domain which are found in a number of cytoskeletal-associated proteins located at the interface between the plasma membrane and the cytoskeleton and in proteins that interact with lipid membranes. The FERM domain is also postulated to bind to adhesion proteins, in a PIP2-regulated fashion, providing a link between cytoskeletal signals and membrane dynamics. Thus TEG protein may play a role in tegument function and interaction with the host.

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“…Paramyosin, sHSP, TSA18, F18, 50 kDa glycoprotein, TsAg5, and other molecules were cloned and expressed in prokaryotic and eukaryotic systems and evaluated with collections of serum and CSF samples. The recombinant products have been checked in ELISA and Western blot, with good sensitivity and specificity for NCC diagnosis (Vazquez-Talavera et al 2001;Ferrer et al 2003bFerrer et al , 2005aFerrer et al , 2007aMontero et al 2003;Hancock et al 2004). Even though most of them worked better with active NCC samples, TSA18 expressed in baculovirus system showed the best sensitivity (60 %) for inactive NCC immunodetection.…”

Section: Immunodiagnosismentioning

confidence: 99%

Taeniosis and Cysticercosis

Ferrer

Gárate

2014

Helminth Infections and Their Impact on Global Public Health

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Taeniosis and cysticercosis are zoonotic diseases produced by Taenia saginata and Taenia solium. The adult tapeworms are parasites of human intestine and show a wide geographical distribution. Taenia asiatica, another tapeworm species, was described in Southeast Asia. The larval stages of these cestodes (metacestodes or cysticerci) cause cysticercosis; T. saginata causes bovine cysticercosis, T. asiatica larvae develop in the pig viscera, and T. solium is able to produce cysticercosis in both pig and man. When the parasite larva invades, the central nervous system (CNS) can provoke neurocysticercosis (NCC), one of the most frequent parasitic infections of human CNS. These diseases continue to cause health problems and livestock industry losses in areas where the parasites are endemic and also in non-endemic regions as a consequence of travel and migrations. There are few symptoms associated with taeniosis; in contrast, NCC (pleomorphic pathology) could be a life-threatening disease, depending on the location, number, stage of cysticerci, and the host immune response. Diagnosis of taeniosis is generally achieved by stool microscopic examinations, and the detection of cysticercosis is generally performed by neuroimaging and immunoassays. Both conventional coprological techniques and immunological assays show limitations, and new diagnostic tools have been developed, more specific and sensitive, such as specific monoclonal antibodies, recombinant antigens, synthetic peptides, and PCR. Considering the clinical impact, veterinary problems, and economic losses derived from taeniosis/cysticercosis, control programs have been implemented. In addition, several vaccine candidates have been characterized that could complement the control measures.

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Taenia solium: characterization of a small heat shock protein (Tsol-sHSP35.6) and its possible relevance to the diagnosis and pathogenesis of neurocysticercosis

Cited by 35 publications

References 62 publications

Nitric oxide contributes to host resistance against experimental Taenia crassiceps cysticercosis

Nitric oxide contributes to host resistance against experimental Taenia crassiceps cysticercosis

The Taenia saginata homologue of the major surface antigen of Echinococcus spp. is immunogenic and 97% identical to its Taenia solium homologue

Taeniosis and Cysticercosis

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