Taenia crassiceps: Effect of normal and immune serum on metacestodes in vitro

Siebert, A.E.; Good, A.H.

doi:10.1016/0014-4894(79)90065-1

Cited by 12 publications

(3 citation statements)

References 15 publications

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“…Early studies on the immune response against this parasite were performed in the late 1970s and early 1980s by Siebert and Good [24, 25]. This work mainly focused on the humoral immune response against T. crassiceps and found that antibodies anti- T. crassiceps cannot be correlated with cytotoxic effects or tegument degradation.…”

Section: The Immune Response To Experimental T Crassiceps Infectimentioning

confidence: 99%

Immunoregulation byTaenia crassicepsand Its Antigens

Peón

Espinoza-Jiménez

Terrazas

2013

BioMed Research International

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Taenia crassiceps is a cestode parasite of rodents (in its larval stage) and canids (in its adult stage) that can also parasitize immunocompromised humans. We have studied the immune response elicited by this helminth and its antigens in mice and human cells, and have discovered that they have a strong capacity to induce chronic Th2-type responses that are primarily characterized by high levels of Th2 cytokines, low proliferative responses in lymphocytes, an immature and LPS-tolerogenic profile in dendritic cells, the recruitment of myeloid-derived suppressor cells and, specially, alternatively activated macrophages. We also have utilized the immunoregulatory capabilities of this helminth to successfully modulate autoimmune responses and the outcome of other infectious diseases. In the present paper, we review the work of others and ourselves with regard to the immune response induced by T. crassiceps and its antigens, and we compare the advances in our understanding of this parasitic infection model with the knowledge that has been obtained from other selected models.

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Section: The Immune Response To Experimental T Crassiceps Infectimentioning

confidence: 99%

Immunoregulation byTaenia crassicepsand Its Antigens

Peón

Espinoza-Jiménez

Terrazas

2013

BioMed Research International

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“…Studies done with other cestodes have shown that a vigorous antibody response is associated with active infection (1,46). The cyst stages of the parasites are resistant to killing by the antibody (38). Instead cysts are shown to actively bind and take up immunoglobulin (4,6,12,15).…”

Section: Discussionmentioning

confidence: 99%

Granuloma cytokines in murine cysticercosis

et al. 1997

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Taenia solium, is one of the most common causes of seizures worldwide. The symptoms result from granulomatous inflammation associated with dying cyst forms of the parasite. Although the invasive larvae can be killed by immune serum plus complement, immunity to the cyst stage depends on a cellular response. This dichotomous immune response is reminiscent of the extremes of the immune response associated with T helper 1 (Th1) and Th2 cytokine profiles. To characterize the cytokine response in cysticercosis, granulomas were removed from the peritoneal cavity of mice infected with Taenia crassiceps cysts and examined for cytokine message by in situ hybridization using 35 S-labeled RNA probes. The granulomas were staged based on histologic appearance of the degenerating parasite. Message for gamma interferon (IFN-␥) was identified by light microscopy in 11 of the 12 granulomas, and interleukin-2 (IL-2) message was identified in 9 of the 12. By laser scanning confocal microscopy, significantly increased IFN-␥ and IL-2 pixel intensity was identified in nearly all of the granulomas from early histologic stages. Message for IL-4 was seen in 6 of the 12 granulomas. Only granulomas with complete destruction of the parasite architecture displayed more than minimal amounts of IL-4 message by light microscopy, and only 2 of 12 granulomas had IL-4 pixel intensity significantly above background. Only minimal amounts of IL-10 message were detected in 4 of 11 granulomas. Thus, early granulomas in cysticercosis are predominantly associated with a Th1 response, whereas later granulomas, in which parasite destruction is complete, have a mixture of Th1 and IL-4. The Th1 response appears to play an important role both in the pathogenesis of disease as well as in the clearing of the parasites, with IL-4 involved in downregulation of the initial response.

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“…It was also observed that if the culture vessel contained less than a critical volume of parasites (2-3 ml) then the survival of parasites in that particular culture was low. This may be due to the fact that the cysticerci may collectively produce a substance which normally inhibits the host immune response (Siebert & Good, 1979) and which may still be required by the cysticerci during the early stages of culture for protection against immune cells still attached to the tegument. Those parasites that were unsuitable for culture became inactive, produced many small buds and eventually shrunk in size until there was a total loss of form.…”

Section: Immunoprecipitation Of Biosynthetically Labelled and Rna Tramentioning

confidence: 99%

Taenia crassicepssurface immunoglobulins: parasite- or host-derived?

McManus

Lamsam

1990

Parasitology

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In common with other taeniid cestodes, host or host-like proteins, especially immunoglobulins, occur on the surface and in the cyst fluid of Taenia crassiceps metacestodes. Here, several approaches have been used to determine the origin of the immunoglobulins present on the tegument. Indirect IFAT showed that IgG was almost totally lost from the surface of bladders after 6 days culture in vitro. There was a rapid reacquisition of immunoglobulins following incubation of the cultured metacestodes with either normal mouse serum or mouse anti-T. crassiceps antiserum. Immunoprecipitation of in vitro translation products and biosynthetically labelled T. crassiceps proteins with a panel of anti-IgG antisera failed to positively identify any molecule with homology to mammalian immunoglobulins. These results suggest strongly that the immunoglobulins located on the surface of T. crassiceps are of host rather than parasite origin. The occurrence of a relatively low abundance receptor in the surface of the bladders, which binds non-specific host immunoglobulin, together with surface-bound specific anti-T. crassiceps antibodies can account for the presence of these host proteins. Freshly obtained bladders and metacestodes cultured in vitro for 6 days were transplanted into naive mice and the survival and development of the resulting parasites compared. In some individual mice there was a decrease in the number and volume of metacestodes and an increase in encapsulated parasites arising from cultured bladders. This was probably not related to the loss of host immunoglobulins from the parasite surface during culture as the reacquisition of these proteins after transplantation is likely to be far more rapid than any immune response could evoke in a naive host.

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Taenia crassiceps: Effect of normal and immune serum on metacestodes in vitro

Cited by 12 publications

References 15 publications

Immunoregulation byTaenia crassicepsand Its Antigens

Immunoregulation byTaenia crassicepsand Its Antigens

Granuloma cytokines in murine cysticercosis

Taenia crassicepssurface immunoglobulins: parasite- or host-derived?

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