Taenia crassiceps: A secretion-substance of low molecular weight leads to disruption and apoptosis of seminiferous epithelium cells in male mice

A Taenia crassiceps metacestode factor enhances ovarian follicle atresia and oocyte degeneration in female mice

“…Zepeda et al (2011b) showed that subcutaneous inoculation of peritoneal fluid or T. crassiceps MF was successfully used and hence this procedure was chosen in this study despite the fact that parasites were implanted intraperitoneally. The increase in the carbohydrate concentration in the Sephadex G-50 MF second peak compared with the HPF suggests that at least half of this concentration could be secreted by intraperitoneal metacestodes.…”

“…Fas and Fas ligand expression, which are the proximal members of the death receptor pathway, have been observed in murine ovarian follicles (Dharma et al, 2003). The physical and chemical characteristics of the low molecular weight (,1.350 kDa) T. crassiceps MF, its absorbance at 260 nm wavelength (Zepeda et al, 2011b) and the presence of an oligosaccharide (Cassani et al, unpublished data) resemble the T. solium MF (Molinari et al, 1990). Apoptosis is accompanied by cell rounding-up, cytoplasmic prolongation retraction, reduced cellular volume, chromatin condensation (pyknosis), nuclear fragmentation (karyorrhexis), plasma membrane blebbing, with maintenance of integrity until the final stages, and engulfment by resident phagocytes in vivo.…”

“…Peritoneal fluid from both healthy (HPF) and T. crassiceps metacestode-infected female mice was obtained following the method of Zepeda et al (2011b). Briefly, after 9 days of infection with T. crassiceps metacestodes, mice were anaesthetized and the peritoneal cavity of each mouse, including control mice, was washed with 8 ml sterile phosphate-buffered saline (PBS) through a small laparotomy.…”

“…To obtain the T. crassiceps MF, lyophilized peritoneal fluids (15 mg dry weight) from infected mice were resuspended in 15 ml PBS, filtered through a 0.22 mm Millipore membrane and loaded on to a Sephadex G-50 M (90 £ 1.5 cm) column (Pharmacia, Sweden) according to the method of Zepeda et al (2011b). Fractions, each of 4 ml, were then collected and the eluted material was monitored at 280 nm.…”

Effects of Taenia Pisiformis Infection and Obesity on Clinical Parameters, Organometry and Fat Distribution in Male Rabbits

“…In addition, it has been observed that patients infected with the protozoa Giardia lamblia present lower levels of total cholesterol and LDL compared to healthy individuals [ 1 ]. In the case of cestodes, specifically in male mice infected with Taenia crassiceps , it was found an increase in the number of macrophages in the luminal space and in the seminiferous tubules, and morphological abnormalities in somatic cells (Sertoli and Leydig cells) and in germ cells [ 10 ]. The increase in visceral adipose tissue is associated with glucose intolerance and insulin resistance, which is directly correlated with the content of triglycerides in the liver [ 11 ].…”