2019
DOI: 10.25083/rbl/24.5/845.850
|View full text |Cite
|
Sign up to set email alerts
|

Tacrolimus concentrations by ELISA and LC - MS/MS

Abstract: Introduction. Tacrolimus has immunosuppressive effects in vitro through the inhibition of mixed lymphocyte reactivity and the generation of cytotoxic T cells. There is a wide variety of laboratory methods for monitoring of immunosuppressive drugs. There is a growing clinical needfor a new evaluation of these methods. Material and Method. A total of 17 transplant patients were retrospectively analyzed. This study aimed to evaluate the concordance between ELISA and LC-MS/MS methods for measurement of tacrolimus … Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1

Citation Types

0
1
0

Year Published

2023
2023
2023
2023

Publication Types

Select...
1

Relationship

0
1

Authors

Journals

citations
Cited by 1 publication
(1 citation statement)
references
References 18 publications
(18 reference statements)
0
1
0
Order By: Relevance
“…However, the above-mentioned highly-accurate and sensitive pieces of equipment are expensive, their operation is time-consuming, and requires highly professional operators because of the complicated detection steps, which makes them unsuitable for the needs of rapid and frequent monitoring. The immunoassay methods used for tacrolimus include enzyme-linked immunosorbent assay (ELISA), 26 microparticle enzyme immunoassay (MEIA), 27 chemiluminescence enzyme immunoassay (CLEIA) 28 and electrochemiluminescence immunoassay (ECLIA). 29 The immunoassay is simple to perform and low cost, but it has the disadvantages of low sensitivity and lack of specificity.…”
Section: Introductionmentioning
confidence: 99%
“…However, the above-mentioned highly-accurate and sensitive pieces of equipment are expensive, their operation is time-consuming, and requires highly professional operators because of the complicated detection steps, which makes them unsuitable for the needs of rapid and frequent monitoring. The immunoassay methods used for tacrolimus include enzyme-linked immunosorbent assay (ELISA), 26 microparticle enzyme immunoassay (MEIA), 27 chemiluminescence enzyme immunoassay (CLEIA) 28 and electrochemiluminescence immunoassay (ECLIA). 29 The immunoassay is simple to perform and low cost, but it has the disadvantages of low sensitivity and lack of specificity.…”
Section: Introductionmentioning
confidence: 99%