TACC3-TSC2 maintains nuclear envelope structure and controls cell division

The Centrosomal Adaptor TACC3 and the Microtubule Polymerase chTOG Interact via Defined C-terminal Subdomains in an Aurora-A Kinase-independent Manner

“…Consistent with previous findings (28,33,55), the N termini of TACC3 isoforms are characterized by a variable length and different amino acid composition as compared with other vertebrate TACC family members. Interestingly, the first 100 amino acids of TACC3 isoforms display a sequence identity of up to 75% (data not shown) followed by the central repeat region that in the case of murine TACC3 comprises seven conserved serine-proline-glutamate-rich repeats (7R) each consisting of 24 amino acids.…”

“…This is different for the TACC domain that has been identified by yeast two hybrid-based screening as well as pulldown and immunoprecipitation assays as major binding partner for various, functionally rather diverse proteins. These include factors involved in cortical neurogenesis (Cep192, DOCK7) (68,69), hematopoietic development (FOG-1) (70), hypoxia response and gene expression (ARNT) (66), transcriptional regulation (MBD2) (71), and regulation of mTOR signaling (TSC2) (55). Interestingly, FOG-1 and ARNT have been proposed to bind to a region containing the last 20 residues of the CC2 subdomain (66,72).…”

“…Further features include a central serine-proline-glutamate-rich repeat region (28,33) that in the case of murine TACC3 is characterized by seven perfect repeats of 24 amino acids each (thereafter referred to as "7R") (33,38) as well as a highly conserved, coiled-coil-containing C terminus (thereafter referred to as "CC" or "TACC domain"). This signature domain is composed of ϳ200 amino acids (aa) (27,33,55), required for centrosomal localization, and known to be involved in protein-protein interaction (28). Here, TACC proteins interact from yeast to human through their TACC domain with the C terminus of XMAP215 family members (28,46,56,57), thereby targeting them to spindle poles.…”

The tumor suppressor tuberin regulates mitotic onset through the cellular localization of cyclin B1

“…Previous work has demonstrated a role for tuberin in regulating nucleocytoplasmic transport. 32,33 Whether interactions with TACC3 are necessary for tuberin effects on CycB1 remain to be determined. It is notable that this report and others 34 have shown an accumulation of CycB1 in tuberin mutants.…”

TACC3 promotes epithelial–mesenchymal transition (EMT) through the activation of PI3K/Akt and ERK signaling pathways

“…All three known human TACC proteins (TACC1-3) share a highly conserved C-terminal coiled-coil domain (TACC domain), which can interact with tubulin/microtubules [14]. Although the role of TACC3 has not been fully elucidated, increasing evidence suggests that TACC3 is required for centrosomedependent microtubule assembly, kinetochore-microtubule attachment and spindle-dependent chromosome alignment during mitosis [17][18][19][20][21][22]. Importantly, mitotic kinase Aurora A-mediated TACC3 phosphorylation is essential for its localization to mitotic spindles and centrosomes in mitotic cells [21,23].…”