TACC3 expression is tightly regulated during early differentiation

Sadek, Christine M.; Pelto‐Huikko, Markku; Tujague, Michel; Steffensen, Knut R.; Wennerholm, M.; Gustafsson, Jan‐Åke

doi:10.1016/s1567-133x(02)00066-2

Cited by 50 publications

(62 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, TACC3 is highly upregulated in specific tissues during development, while TACC1 levels are more widespread [Sadek et al, 2003]. We sought to examine whether Xenopus TACC1 was spatiotemporally regulated during embryonic development, in comparison to TACC3.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Xenopus TACC1 is a microtubule plus‐end tracking protein that can regulate microtubule dynamics during embryonic development

et al. 2015

View full text Add to dashboard Cite

Microtubule plus‐end dynamics are regulated by a family of proteins called plus‐end tracking proteins (+TIPs). We recently demonstrated that the transforming acidic coiled‐coil (TACC) domain family member, TACC3, can function as a +TIP to regulate microtubule dynamics in Xenopus laevis embryonic cells. Although it has been previously reported that TACC3 is the only TACC family member that exists in Xenopus, our examination of its genome determined that Xenopus, like all other vertebrates, contains three TACC family members. Here, we investigate the localization and function of Xenopus TACC1, the founding member of the TACC family. We demonstrate that it can act as a +TIP to regulate microtubule dynamics, and that the conserved C‐terminal TACC domain is required for its localization to plus‐ends. We also show that, in Xenopus embryonic mesenchymal cells, TACC1 and TACC3 are each required for maintaining normal microtubule growth speed but exhibit some functional redundancy in the regulation of microtubule growth lifetime. Given the conservation of TACC1 in Xenopus and other vertebrates, we propose that Xenopus laevis is a useful system to investigate unexplored cell biological functions of TACC1 and other TACC family members in the regulation of microtubule dynamics. © 2015 The Authors. Cytoskeleton, Published by Wiley Periodicals, Inc.

show abstract

Section: Resultsmentioning

confidence: 99%

“…In mammals, TACC1 appears to be more widely‐expressed than TACC3, with TACC1 showing almost ubiquitous expression in adult murine and human tissues [Sadek et al, 2003; Still et al, 1999]. However, TACC1 is also spatiotemporally regulated during murine development [Lauffart et al, 2006; Still et al, 1999].…”

Section: Introductionmentioning

confidence: 99%

Xenopus TACC1 is a microtubule plus‐end tracking protein that can regulate microtubule dynamics during embryonic development

et al. 2015

View full text Add to dashboard Cite

show abstract

“…These genes encode proteins that are highly acidic and contain a conserved 200 amino-acid coiled coil domain, termed 'the TACC domain', which we previously predicted to play a critical role in the function of these proteins (Still et al, 1999a). Both in vitro and in vivo studies indicate that the TACC proteins are functionally linked to the processes of cell growth and differentiation (Gergely et al, 2000;Lauffart et al, 2003;Sadek et al, 2003). In fact, functional deletion of the Tacc3 gene in mice is embryonically lethal, with homozygous knockout mice dying during mid to late gestation (Piekorz et al, 2002).…”

mentioning

confidence: 99%

“…However, although initially described as centrosomal proteins (Gergely et al, 2000;Gergely, 2002), during interphase the human TACC proteins are actually distributed throughout the cell, with TACC1 and TACC3 being primarily concentrated in the nucleus (Gergely et al, 2000). Nuclear accumulation of TACC3 has also been noted during normal mouse development (Aitola et al, 2003;Sadek et al, 2003). Thus, the distribution and behavior of the TACC proteins resembles those of signaling intermediates that shuttle between the cytoplasm and the nucleus, suggesting that TACC proteins may have additional functions outside their potential role at the centrosome and during mitosis.…”

mentioning

confidence: 99%

The transforming acidic coiled coil proteins interact with nuclear histone acetyltransferases

et al. 2004

View full text Add to dashboard Cite

“…6). Expression data on TACC2 and TACC3 are compiled from published data (Still et al, 1999a, b;Sadek et al, 2003;Lauffart et al, 2003;Aitola et al, 2003). (Ϫ, Ϫ/ϩ, ϩϩ, ϩϩϩ, ϩϩϩϩ) represent increasing degrees of expression of the isoform, based upon amplification in the linear range compared to 18S RNA.…”

Section: Differential Expression Of Tacc1 Splice Variants In Adult Humentioning

confidence: 99%

Temporal and spatial expression of TACC1 in the mouse and human

Lauffart¹,

DiMatteo²,

Vaughan³

et al. 2006

Dev. Dyn.

View full text Add to dashboard Cite

TACC1 is the founding member of the evolutionarily conserved transforming acidic coiled coil genes. These genes play a role in normal development and tumorigenesis through interactions with multiple complexes involved in transcription, translation, and centrosomal dynamics. Despite its importance, detailed examination of the expression of TACC1 and splice variants has not previously been performed. In this study, the spatiotemporal distribution of the Tacc1 protein was examined immunohistochemically in cross-sections of mouse embryonic tissues. We also report the distribution of currently known/predicted TACC1 splice variants in adult humans. These results indicate that Tacc1 is regulated in a dynamic manner during embryogenesis. In adult humans, ubiquitous expression of at least one TACC1 splice variant is noted, although specific combinations of variants are evident in individual differentiated tissues. An important observation is that in the in vivo three-dimensional tissue architecture of the growing organism, both the human and mouse TACC1 protein can be localized to different subcellular compartments in a cell-and tissue-specific manner. This indicates that exploration of TACC1 function must take into account the temporal expression of specific splice variants that may perform different cell-type and tissue-specific functions. Furthermore, this analysis will provide the groundwork from which future Tacc1 knockout strategies can be designed and properly interpreted.

show abstract

TACC3 expression is tightly regulated during early differentiation

Cited by 50 publications

References 34 publications

Xenopus TACC1 is a microtubule plus‐end tracking protein that can regulate microtubule dynamics during embryonic development

Xenopus TACC1 is a microtubule plus‐end tracking protein that can regulate microtubule dynamics during embryonic development

The transforming acidic coiled coil proteins interact with nuclear histone acetyltransferases

Temporal and spatial expression of TACC1 in the mouse and human

Contact Info

Product

Resources

About