Tacaribe Virus Infection May Induce Inhibition of the Activity of the Host Cell Ca2+ and Na+/K+ Pumps

Abstract

SUMMARYInfection of Vero cells with Tacaribe virus stocks containing a high ratio of standard (plaque-forming) viruses to defective interfering particles (DIP) induced inhibition of the host cell Ca 2+ ATPase (Ca 2+ pump) and the ouabain-sensitive Na+/K + ATPase (Na+/K + pump). The Mg 2+ ATPase which is not involved in cation transport was not affected. The presence of DIP in the inocula protected the cells from alteration of the transport-associated ATPases induced by standard viruses.

Rotavirus infection alters Na+ and K+ homeostasis in MA-104 cells

“…This conclusion is supported by the observation in infected cells of (i) an increase in the passive 86Rb efflux and 22Na uptake; (ii) a stimulation of the ouabainsensitive 86Rb uptake, indicating a stimulation of the N a t / K ÷ pump; and (iii) no changes in the number of N a t / K + pumps and in the activity of the N a t / K + ATPase. This situation is similar to the one observed in cells infected with picornavirus or cytomegalovirus (Nair, 1984;Lacal & Carrasco, 1982;Nokta et al, 1988) but completely different from the one observed after infection with togavirus or arenavirus (Ulug et al, 1984;Rey et al, 1988), in which ion concentration changes appeared to be related to inhibition of the N a t / K + ATPase activity. In addition, we have found that rotavirus infection increases intracellular free Ca 2÷ concentration with approximately the same time course as the changes observed in monovalent cation homeostasis (Michelangeli et al, 1991).…”

“…This appears to be a rather general phenomenon in lytic infections by either enveloped or naked viruses. The alteration of the intracellular concentrations of Na ÷ and K ÷ has been related to mechanisms such as a change in the plasma membrane permeability to these ions or to an inhibitory effect on Na+/K + ATPase activity (Lop~z-Rivas et al, 1987;Ulug et al, 1984;Rey et al, 1988). This effect is presumed to play a role in the induction of morphological changes in infected cells, such as the development of cytomegaly in cytomegalovirus-infected cells (Nokta et al, 1988), and to contribute to the development of c.p.e.…”

Effects of herpes simplex virus type 1 infection on the plasma membrane and related functions of HeLa S3 cells

“…Membrane-related changes following virus infection have been examined in various virus-cell systems. Reversed polarity has been observed in transmembrane potentials of HEp2 and fibroblast cells (Fritz & Nahmias, 1972) and alterations have been reported in the activities of Na +, K * and Ca 2+ pumps of cells infected with Arenaviridae (Rey et al, 1988), Herpesviridae (Hackstadt & Mallavia, 1982), Reoviridae (del Castillo et al, 1991), Rhabdoviridae (Francouer & Stanners, 1978;Gray et al, 1983), Bunyaviridae (Frugulhetti & Rebello, 1989), Picornaviridae (Carrasco & Smith, 1976;Castrillo & Carrasco, 1986) and Togaviridae (Garry et al, 1979). Although viral gene products have recently been implicated in the selective shut-off of host cell protein synthesis (Wycoff et al, 1992;Kwong & Frankel, 1989), changes in intracellular ion concentrations have been claimed to modulate the function of the eukaryotic ribosome (Carrasco & Smith, 1976;Perez & Carrasco, 1992).…”

Análisis molecular del gen de la proteína de la nucleocapside del virus Junín

“…La composición lipídica de los arenavirus ha sido estudiada en algunos de sus miembros, encontrándose que no tiene la misma composición cuantitativa y cualitativa que la membrana plasmática de las células infectadas por el virus (Rosas, 1984;Rosas et al, 1988). Esta relación entre la brotacion del virus y la membrana plasmática de la célula hospedadora ha dado lugar a diversos estudios acerca de la influencia de la infección viral en los procesos mediados por membranas tales como la variación de la permeabilidad a iones y la disminución de la actividad de las bombas de Na+/K+ y bomba de Ca++ (Rey et al, 1988;Rey, 1990) Las partículas virales son pleomórficas, con un diámetro aproximado de 50 a 300 nm (Dalton et al, 1968;Gschwender et al, 1975;Murphy et al, 1969;Murphy et al, 1970;Murphy y Whitfield, 1975 Se indican las dos formas de nucleocápsides S y L. a. Esquema de la estructura probable del RNA S de un arenavirus (N: gen de la proteína de la nucleocápside; GPC: gen del precursor de las glicoproteínas). Se indica el apareamiento de los extremos 3' y 5' (pandhandle) y la estructura probable de la región intergénica.…”