Tabrizicola alkalilacus sp. nov., isolated from alkaline Lake Dajiaco on the Tibetan Plateau

Phurbu, Dorji; Wang, Hui; Tang, Qian; Lü, Huibin; Zhu, Han; Jiang, Si‐Ping; Xing, Peng; Wu, Qinglong L.

doi:10.1099/ijsem.0.003635

“…The genus Tabrizicola (type species, Tabrizicola aquatica ) proposed by Tarhriz et al [5] and recently emended by Tarhriz et al [6], belongs to the family Rhodobacteraceae . The genus Tabrizicola presently comprises five species with validly published names: Tabrizicola aquatica , isolated from a freshwater lake [5], Tabrizicola fusiformis isolated from a waste-activated sludge [7], Tabrizicola sediminis isolated from the sediment of saline lake [8], Tabrizicola alkalilacus isolated from an alkaline lake [9] and Tabrizicola piscis isolated from the intestinal tract of a freshwater fish [10]. Cells are Gram-stain-negative, aerobic, chemoorganotrophic, rod-shaped, non-motile, catalase-positive and oxidase-positive.…”

Section: Full-textmentioning

confidence: 99%

“…Chemotaxonomically, cells possess ubiquinone-10 (Q-10) as the major respiratory quinone, C 18 : 1 ω7c and C 18 : 1 ω7c 11-methyl as the predominant fatty acids, C 18 : 0 3-OH as the cellular hydroxy fatty acid, and DNA G+C contents between 62.9 and 65.9 mol% [5][6][7][8][9][10].…”

mentioning

confidence: 99%

Tabrizicola oligotrophica sp. nov. and Rhodobacter tardus sp. nov., two new species of bacteria belonging to the family Rhodobacteraceae

Sheu

¹

,

Li

²

,

Sheu

³

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

Two Gram-stain-negative, aerobic, non-motile bacteria, designated KMS-5T and CYK-10T, were isolated from freshwater environments. 16S rRNA gene sequence similarity results indicated that these two novel strains belong to the family Rhodobacteraceae . Strain KMS-5T is closely related to species within the genus Tabrizicola (96.1–96.8 % sequence similarity) and Cypionkella (96.5–97.0 %). Strain CYK-10T is closest to Rhodobacter thermarum YIM 73036T with 96.6 % sequence similarity. Phylogenetic analyses based on 16S rRNA gene sequences and an up-to-date bacterial core gene set showed that strain KMS-5T is affiliated with species in the genus Tabrizicola and strain CYK-10T is placed in a distinct clade with Rhodobacter blasticus ATCC 33485T, Rhodobacter thermarum YIM 73036T and Rhodobacter flagellatus SYSU G03088T. These two strains shared common chemotaxonomic features comprising Q-10 as the major quinone, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine as the principal polar lipids, and C18 : 1 ω7c as the main fatty acid. The average nucleotide identity, average amino acid identity and digital DNA–DNA hybridization values between these two novel isolates and their closest relatives were below the cut-off values of 95–96, 90 and 70 %, respectively, used for species demarcation. The obtained polyphasic taxonomic data suggested that strain KMS-5T represents a novel species within the genus Tabrizicola , for which the name Tabrizicola oligotrophica sp. nov. is proposed with KMS-5T (=BCRC 81196T=LMG 31337T) as the type strain, and strain CYK-10T should represent a novel species of the genus Rhodobacter , for which the name Rhodobacter tardus sp. nov. is proposed with CYK-10T (=BCRC 81191T=LMG 31336T) as the type strain.

show abstract

“…S3). All strains in the genus Tabrizicola contain phosphatidylglycerol, phosphatidylethanolamine, and diphosphatidylglycerol as cellular polar lipids [9–13]. Strain ETT8 T and all reference strains showed Q-10 as a predominant quinone.…”

Section: Full-textmentioning

confidence: 99%

“…Both ANI and dDDH values were far below the cutoff values recommended for bacterial species delineation [33,38]. Furthermore, the AAI cutoff range for classifying strains under the *Data obtained from [12,13]. Table 1.…”

mentioning

confidence: 98%

“…The genus Tabrizicola, belonging to the family Rhodobacteraceae within class Alphaproteobacteria, was introduced by Tarhriz et al [9]. At the time of writing, this genus comprises five recognized species, namely T. aquatica RCRI19 T [9], T. fusiformis SY72 T [10], T. sediminis DRYC-M-16 T [11], T. alkalilacus DJC T [12], and T. piscis K13M18 T [13]. These bacteria are all Gram-stain-negative and non-motile, containing C 18 : 1 ω6c and/or C 18 : 1 ω7c as the major fatty acids and Q-10 as the predominant quinone, plus the DNA G+C contents range from 62.9 mol% to 66.4 mol% [9][10][11][12][13].…”

mentioning

confidence: 99%

See 1 more Smart Citation

Tabrizicola algicola sp. nov. isolated from culture of microalga Ettlia sp.

Park

¹

,

Chun

²

,

Jin

³

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

A novel Gram-stain-negative, aerobic, non-spore-forming, non-motile, and rod-shaped bacterium, strain ETT8T was isolated from a chemostat culture of microalga Ettlia sp. YC001. Optimal growth was with 0–2% NaCl and at 25–37 °C on R2A medium. Phylogenetic analysis based on the 16S rRNA gene and genome sequence showed that strain ETT8T belongs to the genus Tabrizicola , with the close neighbours being T. sediminis DRYC-M-16T (98.1 %), T. alkalilacus DJCT (97.6 %), T. fusiformis SY72T (96.9 %), T. piscis K13M18T (96.8 %), and T. aquatica RCRI19T (96.5 %). The genomic comparison of strain ETT8T with type species in the genus Tabrizicola was analysed using the genome-to-genome distance calculator (GGDC), average nucleotide identity (ANI), and average amino acid identity (AAI) (values indicated ≤17.7, ≤75.4 and ≤71.9 %, respectively). The genomic DNA G+C content of strain ETT8T was 64.4 %, plus C18 : 1 ω6c and C18 : 0-iso were the major fatty acids and Q-10 the major respiratory quinone. Strain ETT8T contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine aminolipid, and four unidentified lipids as the major polar lipids. Based on the chemotaxonomic, genotypic, and phenotype results, strain ETT8T was recognized as a novel species of the genus Tabrizicola for which the name Tabrizicola algicola sp. nov. is proposed. The type strain is ETT8T (=KCTC 72206T=JCM 31893T=MCC 4339T).

show abstract

Rhodobacter amnigenus sp. nov. and Rhodobacter ruber sp. nov., isolated from freshwater habitats

Chen

¹

,

Chang

²

,

Yang

³

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

Two bacterial strains, designated HSP-20T and CCP-1T, isolated from freshwater habitats in Taiwan, were characterized by polyphasic taxonomy. Both strains were Gram-stain-negative, aerobic, non-motile and rod-shaped. Cells of strains HSP-20T and CCP-1T formed pink and dark red coloured colonies, respectively. Both strains contained bacteriochlorophyll a, and showed optimum growth under anaerobic conditions by photoheterotrophy, but no growth by photoautotrophy. Phylogenetic analyses based on 16S rRNA gene and whole-genome sequences indicated that both strains belonged to the genus Rhodobacter . Analysis of 16S rRNA gene sequences showed that strains HSP-20T and CCP-1T shared 98.3 % sequence similarity and were closely related to Rhodobacter tardus CYK-10T (96.0 %) and Rhodobacter flagellatus SYSU G03088T (96.0 %), respectively. Both strains shared common chemotaxonomic characteristics including Q-10 as the major isoprenoid quinone, C18 : 1 ω7c as the predominant fatty acid, and phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine as the main polar lipids. The DNA G+C content of both strains was 66.2 mol%. The average nucleotide identity, average amino acid identity and digital DNA–DNA hybridization values between these two novel isolates and their closest relatives were below the cut-off values of 95–96, 90 and 70 %, respectively, used for species demarcation. On the basis of phenotypic and genotypic properties and phylogenetic inference, both strains should be classified as novel species within the genus Rhodobacter , for which the names Rhodobacter amnigenus sp. nov. (=BCRC 81193T=LMG 31334T) and Rhodobacter ruber sp. nov. (=BCRC 81189T=LMG 31335T) are proposed.

show abstract

Tabrizicola alkalilacus sp. nov., isolated from alkaline Lake Dajiaco on the Tibetan Plateau

Cited by 14 publications

References 30 publications

Tabrizicola oligotrophica sp. nov. and Rhodobacter tardus sp. nov., two new species of bacteria belonging to the family Rhodobacteraceae

Tabrizicola oligotrophica sp. nov. and Rhodobacter tardus sp. nov., two new species of bacteria belonging to the family Rhodobacteraceae

Tabrizicola algicola sp. nov. isolated from culture of microalga Ettlia sp.

Rhodobacter amnigenus sp. nov. and Rhodobacter ruber sp. nov., isolated from freshwater habitats

Contact Info

Product

Resources

About