Tabelle der im Alter auftretenden Kataraktformen - Klinisches Bild, Prognose, Biochemie und Ätiologie

H, Pau

doi:10.1055/s-2008-1050296

Cited by 2 publications

(2 citation statements)

References 6 publications

(8 reference statements)

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“…Netzhaut-Hämangioblastome im Vorpubertäts-und Pubertätsalter Hämangioblastome beim VHL im Kindesalter zwischen zehn und 15 Jahren wurden mitgeteilt von: Amoils und Smith [2], Baras et al [9], Bedell [10], Berblinger [13], Brock et al [18], Browne et al [19], Cordes und Dickson [21], Cordes und Schwartz [22], Czermak [23], Frenkel [31], Gass [36], Ginsberg und Spiro [38], Griffith und Ormond [45], Grossniklaus [46] [112], Pau [115], Rados [118], Röhrborn und Gill [122], Vortmeyer et al [161], Yimoyines et al [174].…”

Section: Die Meisten Patienten Wiesen Im Alter Zwischen Zehn Und 40unclassified

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Angiomatosis retinae

Schmidt¹

2005

Klin Monatsbl Augenheilkd

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Section: Die Meisten Patienten Wiesen Im Alter Zwischen Zehn Und 40unclassified

“…Retinale Hämangioblastome ohne verdickte nutritive Gefäße Pau [115] [175,176] in Frederick (National Cancer Institutes, USA) gemacht: 1993 wurde das VHL-Gen identifiziert [73]. Seither ist es möglich, Einzelpersonen anhand der Blut-DNA hinsichtlich der vorliegenden Mutation zu untersuchen [107].…”

Section: Vorkommen Von Retinalen Hämangioblastomen Im Alterunclassified

Angiomatosis retinae

Schmidt¹

2005

Klin Monatsbl Augenheilkd

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A Cas9-mediated adenosine transient reporter enables enrichment of ABE-targeted cells

et al. 2020

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Background Adenine base editors (ABE) enable single nucleotide modifications without the need for double-stranded DNA breaks (DSBs) induced by conventional CRIPSR/Cas9-based approaches. However, most approaches that employ ABEs require inefficient downstream technologies to identify desired targeted mutations within large populations of manipulated cells. In this study, we developed a fluorescence-based method, named “Cas9-mediated adenosine transient reporter for editing enrichment” (CasMAs-TREE; herein abbreviated XMAS-TREE), to facilitate the real-time identification of base-edited cell populations. Results To establish a fluorescent-based assay able to detect ABE activity within a cell in real time, we designed a construct encoding a mCherry fluorescent protein followed by a stop codon (TGA) preceding the coding sequence for a green fluorescent protein (GFP), allowing translational readthrough and expression of GFP after A-to-G conversion of the codon to “TGG.” At several independent loci, we demonstrate that XMAS-TREE can be used for the highly efficient purification of targeted cells. Moreover, we demonstrate that XMAS-TREE can be employed in the context of multiplexed editing strategies to simultaneous modify several genomic loci. In addition, we employ XMAS-TREE to efficiently edit human pluripotent stem cells (hPSCs), a cell type traditionally resistant to genetic modification. Furthermore, we utilize XMAS-TREE to generate clonal isogenic hPSCs at target sites not editable using well-established reporter of transfection (RoT)-based strategies. Conclusion We established a method to detect adenosine base-editing activity within a cell, which increases the efficiency of editing at multiple genomic locations through an enrichment of edited cells. In the future, XMAS-TREE will greatly accelerate the application of ABEs in biomedical research.

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Tabelle der im Alter auftretenden Kataraktformen - Klinisches Bild, Prognose, Biochemie und Ätiologie

Cited by 2 publications

References 6 publications

Angiomatosis retinae

Angiomatosis retinae

A Cas9-mediated adenosine transient reporter enables enrichment of ABE-targeted cells

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