TAB2, a nucleoside diphosphate protein kinase, is a component of the tMEK2 disease resistance pathway in tomato

Xing, Tim; Rampitsch, Christof; Sun, Shulan; Romanowski, Andrea; Conroy, Chad; Stebbing, Jo‐Ann; Wang, Xiaojing

doi:10.1016/j.pmpp.2008.11.003

Cited by 13 publications

(33 citation statements)

References 44 publications

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“…25 Overexpression of tMEK2 MUT in tomato protoplasts and tomato plants upregulated TAB2. 26 However, a mutation at the kinase interaction motif (KIM) of TAB2 significantly reduced tMEK2 MUT -induced upregulation of PR1b1, β-1,3-glucanase and chitinase genes. 26 Protein pull-down assays suggest that TAB2 interacts with LeMPK3, but not with other members of tomato MAPKs.…”

Section: Nucleoside Diphosphate Protein Kinasementioning

confidence: 98%

“…26 However, a mutation at the kinase interaction motif (KIM) of TAB2 significantly reduced tMEK2 MUT -induced upregulation of PR1b1, β-1,3-glucanase and chitinase genes. 26 Protein pull-down assays suggest that TAB2 interacts with LeMPK3, but not with other members of tomato MAPKs. 26 Overexpression of the wild type TAB2 also enhanced resistance to virulent Pseudomonas syringae pv.…”

Section: Nucleoside Diphosphate Protein Kinasementioning

confidence: 98%

“…26 Protein pull-down assays suggest that TAB2 interacts with LeMPK3, but not with other members of tomato MAPKs. 26 Overexpression of the wild type TAB2 also enhanced resistance to virulent Pseudomonas syringae pv. Tomato.…”

Section: Nucleoside Diphosphate Protein Kinasementioning

confidence: 99%

Section: Prospects In Determining the Precise Mechanisms Of Phosphorymentioning

confidence: 99%

“…In an activated tomato tMEK2 pathway, a phosphoproteomics screening failed to find some known downstream kinases 3,26 such as LeMPK3, which was later identified in an in vitro protein interaction analysis. 26 While the abundance of the kinases or their substrates is one of the key limiting elements in detection, a transient nature of kinase function should be considered carefully. A novel screening platform, quantitative identification of kinase substrates (QIKS), was developed for proteome-wide substrate analysis of specific kinases, 35 and it would be worth testing its applicability in the analysis of plant-pathogen interactions in a defined proteome.…”

Section: Prospects In Determining the Precise Mechanisms Of Phosphorymentioning

confidence: 99%

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Revealing plant defense signaling

Xing¹,

Laroche²

2011

Plant Signaling & Behavior

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Section: Nucleoside Diphosphate Protein Kinasementioning

confidence: 98%

Section: Nucleoside Diphosphate Protein Kinasementioning

confidence: 98%

Section: Nucleoside Diphosphate Protein Kinasementioning

confidence: 99%

Section: Prospects In Determining the Precise Mechanisms Of Phosphorymentioning

confidence: 99%

Section: Prospects In Determining the Precise Mechanisms Of Phosphorymentioning

confidence: 99%

See 3 more Smart Citations

Revealing plant defense signaling

Xing¹,

Laroche²

2011

Plant Signaling & Behavior

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Comparative analysis of constitutive proteome between resistant and susceptible tomato genotypes regarding to late blight

Laurindo

Fontes

et al. 2017

Funct Integr Genomics

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Late blight is one of the most destructive diseases of the tomato, resulting in substantial economic losses. There is difficulty in controlling this disease, so the molecular characterization of tomato genotypes may help in the selection of higher resistance tomato plants against Phytophthora infestans, late blight's pathogen. The objective was to analyze the differences with regard to the constitutive proteome between the access Vegetable Germplasm Bank (BGH)-2127, resistant genotype, and Santa Clara-susceptible genotype to late blight. Proteomic analysis of leaf samples by two-dimensional electrophoresis (2-DE) followed by identification by mass spectrometry (MALDI TOF/TOF) was performed. Nineteen proteins were identified, which were then related to metabolism and energy, photosynthesis, transcription, stress, and defenses. Approximately 90% of these proteins were more abundant in Santa Clara, a susceptible cultivar. Acidic 26 kDa endochitinase and ribonuclease T2 proteins were more abundant in BGH-2127 access. The enzymatic activity confirmed a greater abundance of chitinase in the BGH-2127 access as compared to the cultivar Santa Clara. Gene expression analyses by real-time PCR demonstrated that the mRNA levels were not correlated with the respective protein levels. Abundance of the acidic 26 kDa endochitinase and ribonuclease T2 proteins in the constitutive proteomes of BGH-2127 may be associated with the answer to the resistance of this access.

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TaFLRS, a novel mitogen-activated protein kinase in wheat defence responses

et al. 2011

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Plants respond to biotic and abiotic stresses through the activation and coordination of various signalling pathways. The activation often requires the phosphorylation of proteins. In this study, we have identified the wheat TaFLRS MAP kinase (Fusarium and Leaf Rust Sensitive) gene that was upregulated in a wheat EST (expressed sequence tag) array analysis following a wheat-leaf rust interactive challenge. Our results demonstrate that TaFLRS is transcriptionally upregulated in incompatible interactions involving wheat and leaf rust and Fusarium graminearum, suggesting that this MAPK maybe involved in defence responses to these wheat pathogens. RT-PCR revealed that TaFLRS transcript levels are not altered by salicylic acid (SA) treatment. However, immunoprecipitation and western blotting analysis show that phosphorylation of TaFLRS at the TEY motif was enhanced by SA in the Fusarium head blight (FHB) resistant cultivar Frontana following challenge with the FHB pathogen. The role of TaFLRS MAP kinase in defence responses in wheat is discussed.

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TAB2, a nucleoside diphosphate protein kinase, is a component of the tMEK2 disease resistance pathway in tomato

Cited by 13 publications

References 44 publications

Revealing plant defense signaling

Revealing plant defense signaling

Comparative analysis of constitutive proteome between resistant and susceptible tomato genotypes regarding to late blight

TaFLRS, a novel mitogen-activated protein kinase in wheat defence responses

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