T98G: An anchorage‐independent human tumor cell line that exhibits stationary phase G1 arrest in vitro

Abstract

T98 and T98G are two related cell lines that were derived from a human glioblastoma multiforma tumor. T98G has almost twice as many chromosomes as T98, suggesting that it is a polyploid variant of T98. Three aspects of control of cellular proliferation were studied in T98 and T98G cells in comparison to WI-38 normal human diploid cells. WI-38 cells have the following properties: (1) they can undergo only a limited number of population doublings in vitro; (2) they cannot proliferate without anchorage; and (3) they become arrested in G1 phase under stationary phase conditions. T98 cells differ from normal cells in all three of these properties, as do many other transformed cell lines. However, the derivative of T98, namely T98G, expresses an unique combination of normal and transformed aspects of the control of cellular proliferation. T98G cells are like normal cells in that they become arrested in G1 phase under stationary phase conditions, yet they also exhibit the transformed characteristics of anchorage independence and immortality. Thus, T98G cells demonstrate that transformation to immortality and anchorage independence can exist without concomitant loss of the normal mechanism for G1 arrest in response to stationary phase conditions. This result supports the hypothesis that each of these three aspects of control of cellular proliferation can be altered independently. Partially transformed cell lines, such as T98G, should be useful for sorting out the biochemical changes associated with transformation in each of these aspects.

Suppression of the cell proliferation and invasion phenotypes in glioma cells by the LGI1 gene

“…The GBM-derived T98G (Stein, 1979) and A172 (Giard et al, 1973) cell lines, and the grade III astocytoma-derived U87 cell line (Ponten and Macintyre, 1968) were maintained in DMEM with 10% FBS under 10% CO 2 . Cells were transferred using trypsin/EDTA.…”

p27Kip 1 Expression by Contact Inhibition as a Prognostic Index of Human Glioma

“…Our results indicate that p27 mRNA expression is markedly induced at high cell density in low-grade (T98G and NAC6) but not high-grade (A172 and U251) glioma cells. T98G cells have been suggested to have arisen from T98 cells, which were originally derived from a high-grade glioma; however, the former have retained some normal characteristics such as G1 phase arrest under stationary phase conditions and were found to be nontumorigenic in nude mice (Stein, 1979). Therefore, we evaluated T98G cells as representative of low-grade gliomas.…”

Pre-existing herpes simplex virus 1 (HSV-1) immunity decreases, but does not abolish, gene transfer to experimental brain tumors by a HSV-1 vector

“…46 The T98G human glioma line was established from a human glioblastoma. 47 Tumor cells were grown in Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal calf serum, 100 U/ml penicillin, and 100 g/ml streptomycin (GIBCO, Gaithersburg, MD, USA) at 37°C in 5% carbon dioxide.…”