T2 Magnetic Resonance: A Diagnostic Platform for Studying Integrated Hemostasis in Whole Blood—Proof of Concept

Skewis, Lynell R.; Lebedeva, Tatiana; Papkov, V G; Thayer, Edward C.; Massefski, Walter; Cuker, Adam; Nagaswami, Chandrasekaran; Litvinov, Rustem I.; Kowalska, M. Anna; Rauova, Lubica; Poncz, Mortimer; Weisel, John W.; Lowery, Thomas J.; Cines, Douglas B.

doi:10.1373/clinchem.2014.223735

Cited by 29 publications

(31 citation statements)

References 32 publications

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“…By exploiting magnetic nanoparticles coupled with specific antibodies, low levels of infectious agents can be detected using benchtop T 2 measurements [55,56]. Related technology is being developed for the clinical evaluation of hemostasis, namely disorders of clotting and bleeding [57]. These new applications of compact NMR relaxometry are the focus of another article in this issue authored by Thomas J. Lowery.…”

Section: Low-field Relaxometry Studies Of the Liquid Components Ofmentioning

confidence: 99%

Compact NMR relaxometry of human blood and blood components

Cistola

Robinson

2016

TrAC Trends in Analytical Chemistry

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Nuclear magnetic resonance relaxometry is a uniquely practical and versatile implementation of NMR technology. Because it does not depend on chemical shift resolution, it can be performed using low-field compact instruments deployed in atypical settings. Early relaxometry studies of human blood were focused on developing a diagnostic test for cancer. Those efforts were misplaced, as the measurements were not specific to cancer. However, important lessons were learned about the factors that drive the water longitudinal (T1) and transverse (T2) relaxation times. One key factor is the overall distribution of proteins and lipoproteins. Plasma water T2 can detect shifts in the blood proteome resulting from inflammation, insulin resistance and dyslipidemia. In whole blood, T2 is sensitive to hemoglobin content and oxygenation, although the latter can be suppressed by manipulating the static and applied magnetic fields. Current applications of compact NMR relaxometry include blood tests for candidiasis, hemostasis, malaria and insulin resistance.

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Section: Low-field Relaxometry Studies Of the Liquid Components Ofmentioning

confidence: 99%

Compact NMR relaxometry of human blood and blood components

Cistola

Robinson

2016

TrAC Trends in Analytical Chemistry

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“…of detecting a variety of targets, including: molecular targets (e.g., DNA); immunodiagnostics (e.g., proteins); and a broad range of hemostasis markers [21][22][23][24]. For molecular and immunodiagnostic targets, T2MR utilizes advances in the field of direct sample detection by deploying superparamagnetic particles that enable T2MR signals to be sensitive to specific targets.…”

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confidence: 99%

T2MR and T2Candida: novel technology for the rapid diagnosis of candidemia and invasive candidiasis

2015

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Candidemia and other forms of invasive candidiasis pose a significant diagnostic challenge. In order to provide the best treatment, it is important to accurately detect the fungal infection and identify the species. Historically, diagnosis of Candida infections depended upon three classical laboratory approaches: microbiologic, immunologic, histopathologic; and now includes new methods such as radiographic techniques, molecular, proteomic and biochemical methods. The T2Candida Panel has introduced a new class of infectious disease diagnostics that can rapidly detect and identify the causative pathogen of sepsis directly from a patient blood sample in a culture-independent manner. This test enables detection of Candida directly from the patient sample, a significant advance for the rapid and accurate diagnosis of invasive candidiasis.

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“…T2MR measures the transverse relaxation time of the nuclear magnetic resonance signal of water and is sensitive to changes in the microenvironments in aqueous solutions. 264 Recently, DNA functionalized nanoparticles that cluster upon binding of target nucleic acids were reported, resulting in easily detected T2 relaxation rate changes. 265 In combination with a specially designed, portable T2MR instrument that is compatible with standard PCR tubes, detection of Candida species at concentrations <5 CFU/mL in blood directly from patient samples was reported.…”

Section: Diagnostic Techniquesmentioning

confidence: 99%

Targeted treatment for bacterial infections: prospects for pathogen-specific antibiotics coupled with rapid diagnostics

Maxson

Mitchell

2016

Tetrahedron

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Antibiotics are a cornerstone of modern medicine and have significantly reduced the burden of infectious diseases. However, commonly used broad-spectrum antibiotics can cause major collateral damage to the human microbiome, causing complications ranging from antibiotic-associated colitis to the rapid spread of resistance. Employing narrower spectrum antibiotics targeting specific pathogens may alleviate this predicament as well as provide additional tools to expand an antibiotic repertoire threatened by the inevitability of resistance. Improvements in clinical diagnosis will be required to effectively utilize pathogen-specific antibiotics and new molecular diagnostics are poised to fulfill this need. Here we review recent trends and the future prospects of deploying narrower spectrum antibiotics coupled with rapid diagnostics. Further, we discuss the theoretical advantages and limitations of this emerging approach to controlling bacterial infectious diseases.

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T2 Magnetic Resonance: A Diagnostic Platform for Studying Integrated Hemostasis in Whole Blood—Proof of Concept

Cited by 29 publications

References 32 publications

Compact NMR relaxometry of human blood and blood components

Compact NMR relaxometry of human blood and blood components

T2MR and T2Candida: novel technology for the rapid diagnosis of candidemia and invasive candidiasis

Targeted treatment for bacterial infections: prospects for pathogen-specific antibiotics coupled with rapid diagnostics

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