T lymphocytes possess the machinery for 5‐<scp>HT</scp> synthesis, storage, degradation and release

Chen, Y.; Leon‐Ponte, Matilde; Pingle, Sandeep C.; O’Connell, Peta J.; Ahern, Gerard P.

doi:10.1111/apha.12470

Cited by 37 publications

(41 citation statements)

References 27 publications

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“…As discussed earlier, four enzymes are involved in the synthesis of melatonin: TPH, AADC, AA‐NAT (also called as NAT), and ASMT (also called as HIOMT). It has been reported that TPH and AADC in T cells also participate in serotonin formation . In addition to TPH and AADC, T cells also express NAT and HIOMT.…”

Section: Melatonin Signaling In T Cellsmentioning

confidence: 99%

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Melatonin signaling in T cells: Functions and applications

Ren

Liu

Chen

et al. 2017

Journal of Pineal Research

180

130

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Melatonin affects a variety of physiological processes including circadian rhythms, cellular redox status, and immune function. Importantly, melatonin significantly influences T-cell-mediated immune responses, which are crucial to protect mammals against cancers and infections, but are associated with pathogenesis of many autoimmune diseases. This review focuses on our current understanding of the significance of melatonin in T-cell biology and the beneficial effects of melatonin in T-cell response-based diseases. In addition to expressing both membrane and nuclear receptors for melatonin, T cells have the four enzymes required for the synthesis of melatonin and produce high levels of melatonin. Meanwhile, melatonin is highly effective in modulating T-cell activation and differentiation, especially for Th17 and Treg cells, and also memory T cells. Mechanistically, the influence of melatonin in T-cell biology is associated with membrane and nuclear receptors as well as receptorindependent pathways, for example, via calcineurin. Several cell signaling pathways, including ERK1/2-C/EBPα, are involved in the regulatory roles of melatonin in Tcell biology. Through modulation in T-cell responses, melatonin exerts beneficial effects in various inflammatory diseases, such as type 1 diabetes, systemic lupus erythematosus, and multiple sclerosis. These findings highlight the importance of melatonin signaling in T-cell fate determination, and T cell-based immune pathologies. K E Y W O R D Smelatonin, multiple sclerosis, systemic lupus erythematosus, T cells, Th17 cells, Treg cells

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Section: Melatonin Signaling In T Cellsmentioning

confidence: 99%

“…It has been reported that TPH and AADC in T cells also participate in serotonin formation. 92 In addition to TPH and AADC, T cells also express NAT and HIOMT. Resting PBMCs and PBMCs activated by PHA express NAT and HIOMT and release abundant amounts of melatonin into the culture media.…”

Section: Melatonin Signaling In T Cellsmentioning

confidence: 99%

Melatonin signaling in T cells: Functions and applications

Ren

Liu

Chen

et al. 2017

Journal of Pineal Research

180

130

View full text Add to dashboard Cite

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“…Important genes involved in oxidative stress and detoxification of oxidation by-products are included in this pathway, such as membrane-bound microsomal glutathione S-transferase ( MGST1 ), with a role in the development of inflammation and in cellular defense [101], aldehyde dehydrogenase ( ALDH3B1 ) [102] and monoamine oxidase A ( MAOA ) [103]. Monoamine oxidase A has a role in the metabolism of serotonin: this molecule has been shown to be synthesized, released and degraded also by T lymphocytes [104]). Among the significant genes, we found also some terms significantly overexpressed in other pathways, as AOX1 and CYP2E1 .…”

Section: Discussionmentioning

confidence: 99%

Breed and adaptive response modulate bovine peripheral blood cells’ transcriptome

Pošćić

Montanari

D’Andrea

et al. 2017

J Animal Sci Biotechnol

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BackgroundAdaptive response includes a variety of physiological modifications to face changes in external or internal conditions and adapt to a new situation. The acute phase proteins (APPs) are reactants synthesized against environmental stimuli like stress, infection, inflammation.MethodsTo delineate the differences in molecular constituents of adaptive response to the environment we performed the whole-blood transcriptome analysis in Italian Holstein (IH) and Italian Simmental (IS) breeds. For this, 663 IH and IS cows from six commercial farms were clustered according to the blood level of APPs. Ten extreme individuals (five APP+ and APP- variants) from each farm were selected for the RNA-seq using the Illumina sequencing technology. Differentially expressed (DE) genes were analyzed using dynamic impact approach (DIA) and DAVID annotation clustering. Milk production data were statistically elaborated to assess the association of APP+ and APP- gene expression patterns with variations in milk parameters.ResultsThe overall de novo assembly of cDNA sequence data generated 13,665 genes expressed in bovine blood cells. Comparative genomic analysis revealed 1,152 DE genes in the comparison of all APP+ vs. all APP- variants; 531 and 217 DE genes specific for IH and IS comparison respectively. In all comparisons overexpressed genes were more represented than underexpressed ones. DAVID analysis revealed 369 DE genes across breeds, 173 and 73 DE genes in IH and IS comparison respectively. Among the most impacted pathways for both breeds were vitamin B6 metabolism, folate biosynthesis, nitrogen metabolism and linoleic acid metabolism.ConclusionsBoth DIA and DAVID approaches produced a high number of significantly impacted genes and pathways with a narrow connection to adaptive response in cows with high level of blood APPs. A similar variation in gene expression and impacted pathways between APP+ and APP- variants was found between two studied breeds. Such similarity was also confirmed by annotation clustering of the DE genes. However, IH breed showed higher and more differentiated impacts compared to IS breed and such particular features in the IH adaptive response could be explained by its higher metabolic activity. Variations of milk production data were significantly associated with APP+ and APP- gene expression patterns.Electronic supplementary materialThe online version of this article (doi:10.1186/s40104-017-0143-y) contains supplementary material, which is available to authorized users.

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“…The association of serotonin receptors through kinase signaling is a new perspective, with which to continue studying the fluoxetine effect on TauT. T cells can release 5-HT into the extracellular space in response to stimulation [50] . Therefore, it is possible that 5-HT was present in the microenvironment and exerted some effects on its receptors.…”

Section: Discussionmentioning

confidence: 99%

Fluoxetine Treatment in Rats Increases the Rate of Taurine Transport in Mononuclear Cells

Colmenares-Aguilar

Urbina²,

Obregón³

et al. 2017

Neuroimmunomodulation

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Fluoxetine, a selective serotonin reuptake inhibitor antidepressant, modulates the mitogen-induced proliferation of lymphocytes. Lymphocytes contain taurine and express taurine transporter (TauT). Among the effects of taurine on lymphocytes are protection against oxidants and regulation of the inflammatory aspects of the immune response. Our aim was to determine the influence of fluoxetine treatment on taurine transport, and to determine the presence of TauT in the mononuclear cells of rats. Methods: Male adult Sprague-Dawley rats were treated with fluoxetine 10 mg/kg i.p. for 1, 2, and 3 weeks. The cells were obtained by density gradients. [³H]Taurine was used for transport assays. Amino acid levels were determined by high-performance liquid chromatography. Immunolabeling of CD4+, CD8+, and TauT was performed. The mRNA of TauT was evaluated by RT-PCR. Controls were included for each protocol. Results: The transport of taurine, after 1 week of treatment, was significantly augmented compared to controls. The affinity significantly increased at 1 and 2 weeks. While the percentage of CD4+ cells decreased and that of CD8+ cells increased, the percentage of TauT in CD4+ and CD8+ cells was not affected. Reduction of levels of aspartic acid, glutamic acid, threonine, alanine, glycine, and arginine occurred at 1 and 2 weeks. The taurine concentration significantly decreased after 2 and 3 weeks of treatment. The estimation of mRNA of TauT was not different. Conclusion: Taurine transport increases with fluoxetine treatment, and so this could be related to an immunomodulatory role of fluoxetine through TauT. Inhibition of serotonin reuptake might be involved in the regulation of taurine transport in mononuclear cells.

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T lymphocytes possess the machinery for 5‐HT synthesis, storage, degradation and release

Abstract: These data reveal coordinated changes in 5-HT production, metabolism and storage that may optimize 5-HT secretion from the CD8(+) T cell subset in response to activation stimuli.

Cited by 37 publications

References 27 publications

Melatonin signaling in T cells: Functions and applications

Melatonin signaling in T cells: Functions and applications

Breed and adaptive response modulate bovine peripheral blood cells’ transcriptome

Fluoxetine Treatment in Rats Increases the Rate of Taurine Transport in Mononuclear Cells

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