T-Lymphocytes in Rat Lymphoid Follicles are a Subset of T Helper Cells

Kroese, Franciscus; Wubbena, As; Joling, P.; Nieuwenhuis, Paul

doi:10.1007/978-1-4613-2463-8_54

Cited by 10 publications

(8 citation statements)

References 12 publications

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“…The antibodies were kindly donated by J. Rozing (TNO, Rijswijk, Netherlands). They have been characterized previously (17)(18)(19)(20). Their reaction pattern was confirmed in our laboratory on rat spleen and lymph node sections.…”

Section: Methodsmentioning

confidence: 98%

Transplantation of Purified Islet Cells in Diabetic Rats: II. Immunogenicity of Allografted Islet β-Cells

et al. 1991

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This study examines whether the survival of allografted rat islet beta-cells is influenced by the presence of other pancreatic donor cells. Grafts (RT1u/l) of different cellular composition were intraportally transplanted in streptozocin-induced diabetic rats (RT1n/n). All grafts corrected the diabetic state within 3 days. Implants of freshly isolated islets contained various endocrine and nonendocrine cell types; they became diffusely infiltrated within 1 wk and were completely destroyed within 2 wk. A 4-day culture period did not lead to major changes in the cellular composition of the islets or in their survival as allograft. Islet cell aggregates prepared after islet dissociation and cell purification were less acutely infiltrated and less rapidly rejected. Aggregates composed of sorted MHC class II-negative cells maintained basal normoglycemia in 3 of 5 recipients for 5 wk but only in 1 of 5 for 20 wk. Aggregates of purified islet beta-cells remained relatively free of diffuse infiltrations during the 1st wk and preserved the normalized state in 7 of 13 recipients for 5 wk; after 20 wk, 6 of 13 were still aglucosuric, but 40% of the implants were diffusely infiltrated and depleted of insulin. Reaggregation of purified islet beta-cells with purified islet endocrine non-beta-cells promoted their long-term survival as allograft: 11 of 13 recipients of mixed islet endocrine cells maintained normal basal glycemia over 20 wk; their implants contained relatively constant insulin reserves and remained virtually devoid of diffuse infiltrations. These results demonstrate that techniques aiming at the elimination of surface MHC class II-positive cells are less successful in preparing rat islet allografts of low immunogenicity than methods of positive cell selection. Pure islet beta-cells are immunogenic as an allograft but illicit a milder and less-acute immune attack than undissociated islet tissue. Nonendocrine and damaged islet cells are suspected of enhancing the rapidity and intensity of the cytotoxic reaction. Survival of allografted beta-cells is markedly prolonged by the presence of islet endocrine non-beta-cells within the graft. The mechanisms underlying this effect have not yet been elucidated; they may involve immune and metabolic interactions of the endocrine non-beta-cells. We conclude that purification of islet endocrine cells represents a new and powerful method for preparing insulin-producing allografts that can survive in hosts without pharmacological immunosuppression.

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Section: Methodsmentioning

confidence: 98%

Transplantation of Purified Islet Cells in Diabetic Rats: II. Immunogenicity of Allografted Islet β-Cells

et al. 1991

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“…Sections were scored for mononuclear and polymorph nuclear cells. The other half of the material was snap frozen, and cryostat sections (6-8 pm) were prepared for histochemical examination as described by Kroese et al (16). B-Lymphocytes were identified by staining with rabbit anti-swine IgG (RASw-IgG).…”

Section: Skin Testsmentioning

confidence: 99%

An evaluation of immune competence in different swine breeds

Joling

Mok

Reilingh

et al. 1993

Veterinary Quarterly

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ORIGINAL PAPERS current state of fasting. The correlation coefficients (Table 4) showed that, compared to animals infected with Tvivax, animals infected with Tcongolense with a greatly decreased DMI showed a smaller increase in plasma urea, but a greater increase in plasma NEFA and BHBA. In addition, plasma TP levels were lower in animals Infected with Tcongolense than in animals infected with Various characteristics of the KLH-specific immune response were studied in 988 sows of four breeds. KLHspecific immune responses showed considerable variability. The applied statistical model explained 63 to 73 per cent of this variation. influences, expressed as the heritability estimate, were rather high for the IgG response (0.33), as well as for the skin reaction (0.26) and the LST (0.41 -0.45). A positive correlation between the various immune parameters was found. Selective breeding for immune responsiveness seems to be feasible, but selection for cellular as well as

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“…Firstly, FDC are restricted to B cell follicles, areas that contain only small and possibly selected subsets of T cells (15)(16)(17). Secondly, current knowledge of antigen handling by FDC would indicate that they do not process antigen.…”

Section: Introductionmentioning

confidence: 99%