T‐lymphocyte subpopulation changes during hemolysis in glucose‐6‐phosphate dehydrogenase (G6PD)‐deficient children

Schilirò, Gino; Minniti, Caterina P.; Sciotto, A.; Bellino, A.; Russo, Antonio

doi:10.1002/ajh.2830210206

Cited by 3 publications

(3 citation statements)

References 7 publications

(6 reference statements)

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“…One HIV-positive patient (ID no. 20020282) had a CFP-10 decrease that rebounded 2 mo after completion of therapy, perhaps owing to a lack of leukocyte bactericidal activity associated with G6PD deficiency or a decrease in the proportion of CD4 + T lymphocytes (32). All of the other HIV-positive patients achieved Mtb antigen clearance during treatment or showed continued decreases posttreatment and ultimately had undetectable antigen levels.…”

Section: Longitudinal Quantification Of Cfp-10 and Esat-6 In Patientsmentioning

confidence: 99%

Quantification of circulating Mycobacterium tuberculosis antigen peptides allows rapid diagnosis of active disease and treatment monitoring

Liu

Zhao

Fan

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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Tuberculosis (TB) is a major global health threat, resulting in an urgent unmet need for a rapid, non-sputum-based quantitative test to detect active Mycobacterium tuberculosis (Mtb) infections in clinically diverse populations and quickly assess Mtb treatment responses for emerging drug-resistant strains. We have identified Mtb-specific peptide fragments and developed a method to rapidly quantify their serum concentrations, using antibody-labeled and energy-focusing porous discoidal silicon nanoparticles (nanodisks) and high-throughput mass spectrometry (MS) to enhance sensitivity and specificity. NanoDisk-MS diagnosed active Mtb cases with high sensitivity and specificity in a case-control study with cohorts reflecting the complexity of clinical practice. Similar robust sensitivities were obtained for cases of culture-positive pulmonary TB (PTB; 91.3%) and extrapulmonary TB (EPTB; 92.3%), and the sensitivities obtained for culture-negative PTB (82.4%) and EPTB (75.0%) in HIV-positive patients significantly outperformed those reported for other available assays. NanoDisk-MS also exhibited high specificity (87.1-100%) in both healthy and high-risk groups. Absolute quantification of serum Mtb antigen concentration was informative in assessing responses to antimycobacterial treatment. Thus, a NanoDisk-MS assay approach could significantly improve the diagnosis and management of active TB cases, and perhaps other infectious diseases as well.D espite international efforts and initiatives, tuberculosis (TB) remains a major public health concern worldwide, associated with high morbidity and mortality (1, 2). Detecting active TB cases and monitoring their responses to therapy are fraught with challenges, relying predominantly on microbiologic techniques that use sputum samples, including acid-fast Bacillus (AFB) smear microscopy-widely used as an initial test for TB diagnosis (3, 4)-and Mycobacterium tuberculosis (Mtb) culture, both of which have only moderate sensitivity and specificity and a long turnaround time (5, 6). Moreover, sputum samples are difficult to obtain after symptom improvement, and often are not diagnostically useful for extrapulmonary TB (EPTB) cases. The PCR-based Xpert MTB/RIF sputum assay was introduced to improve the speed and specificity of TB diagnosis, but this assay has poor sensitivity under low bacterial loads and cannot distinguish live and nonviable Mtb contributions (7,8). A recent World Health Organization (WHO) policy update acknowledged the low quality of evidence supporting the use of Xpert MTB/RIF to diagnose EPTB (9). Diagnostic challenges can be further magnified in patients coinfected with HIV and TB (10). In addition, none of these techniques provides quantitative results that can be used to monitor treatment effects (11,12).Consequently, there is an urgent need, highlighted as a high priority in a recent WHO consensus report (13), for the development of rapid, quantitative, non-sputum-based biomarker tests that do not require bacterial isolation to detect active TB (13). Non-s...

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Section: Longitudinal Quantification Of Cfp-10 and Esat-6 In Patientsmentioning

confidence: 99%

Quantification of circulating Mycobacterium tuberculosis antigen peptides allows rapid diagnosis of active disease and treatment monitoring

Liu

Zhao

Fan

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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“…In red blood cells, deleterious variants in G6PD lead to impairment of the pentose phosphate pathway and subsequently the ability of the cell to scavenge free radicals. Although G6PD deficiency is primarily recognized as a disorder of hemolysis, early studies also have reported changes in T cell subpopulations during hemolysis 90 which was proposed to be due to bilirubin and hemoglobin altering the T cell receptor binding characteristics. 91 However, further work on the function of these T cell subpopulations and their relationship to G6PDH deficiency have not performed and may be fruitful in revealing a mechanism of T cell dysfunction.…”

Section: Natural Killer T (Nkt) Cells Have Features Of Both T Cells Andmentioning

confidence: 99%

Chemical individuality in T cells: A Garrodian view of immunometabolism

McGuire

2020

Immunological Reviews

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Metabolically quiescent T cells circulate throughout the body in search of antigen.Following engagement of their cognate receptors, T cells undergo metabolic reprogramming to support their activation, differentiation, and ultimately function. In the spirit of Sir Archibald Garrod, this metabolic reprogramming actually imparts a chemical individuality which confers advantage, while in others confers vulnerability, depending upon the milieu. Studying T cell immunometabolism in the context of inborn errors of metabolism allows one to define essential pathways of intermediary metabolism as well metabolic vulnerabilities and plasticity. Inborn errors of metabolism, a class of diseases first named by Garrod, have a long history of being informative for common physiologic and pathologic processes. This endeavor may be accomplished through the study of patients, animal models, and in vitro models of inborn errors of metabolism. In this review, the basics of intermediary metabolism and core metabolic pathways will be discussed, along with their relationship to T cell immunometabolism.Due to their pleiotropic nature, the reader will be specifically directed toward various inborn errors of metabolism which may be helpful for answering important questions about the role of metabolism in T cells. K E Y W O R D Simmunometabolism, inborn errors of metabolism, T cells

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“… 8 Glucose-6-phosphate dehydrogenase deficiency also affects monocyte/macrophage polarization in humans. 9 Schilirò et al 10 reported that the T-lymphocyte subpopulation changes and the ratio of CD4/8 during hemolysis in children with G6PD deficiency reverses.…”

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confidence: 99%

Evaluation of Lymphocyte Subgroups in Children with Glucose-6- Phosphate Dehydrogenase Deficiency

et al. 2023

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T‐lymphocyte subpopulation changes during hemolysis in glucose‐6‐phosphate dehydrogenase (G6PD)‐deficient children

Cited by 3 publications

References 7 publications

Quantification of circulating Mycobacterium tuberculosis antigen peptides allows rapid diagnosis of active disease and treatment monitoring

Quantification of circulating Mycobacterium tuberculosis antigen peptides allows rapid diagnosis of active disease and treatment monitoring

Chemical individuality in T cells: A Garrodian view of immunometabolism

Evaluation of Lymphocyte Subgroups in Children with Glucose-6- Phosphate Dehydrogenase Deficiency

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