T-lymphocyte responses to plicatic acid–human serum albumin conjugate in occupational asthma caused by western red cedar☆☆☆★★★

Immunoglobulin E-mediated sensitization to pine and beech dust in relation to wood dust exposure levels and respiratory symptoms in the furniture industry

“…Our results suggest that sIgE-mediated mechanisms explain, at the most, a minor part of the increased occurrence of respiratory symptoms found in woodworkers, which is in line with results from, for example, western red cedar (20,21), where a non-IgE-mediated specific effect of plicatic acid is very likely. We have earlier investigated IgE-mediated sensitization by skin prick test for both high molecular weight (HMW) (>10 kDa) and LMW extract (<10 kDa) of pine and beech dust (40), and we found few positive skin prick test for the low compared to the HMW extracts, which do not support IgE-mediated sensitization to LMW agents to be of major importance, at least for pine and beech.…”

“…The aetiological agent is the low molecular weight (LMW) agent plicatic acid (18). In a single study, specific IgE (sIgE) was documented among 44% (8 out of 18) with a positive specific provocation test for plicatic acid (19), but further studies have shown IgE-mediated sensitization to be of minor importance for the aetiology of western red cedar asthma ( 20,21).…”

Subclinical immunologic and physiologic responses in hexamethylene diisocyanate‐exposed auto body shop workers

“…The means of quadruplicate cultures were determined and the results expressed as stimulation indices which were calculated based on (average cpm with test antigenÐaverage cpm background)/(average cpm with control antigenÐaverage cpm background). A stimulation index of 2.0 was regarded as the cutoff criterion for a positive response, a cutoff used by other investigators [Zeiss et al, 1977;Frew et al, 1998]. …”

Diisocyanate‐exposed auto body shop workers: A one‐year follow‐up

“…The means of quadruplicate cultures were determined and the results expressed as stimulation indices which were calculated based on (average cpm with test antigenÀaverage cpm background)/(average cpm with control antigenÀaverage cpm background). A stimulation index of 2.0 was regarded as the cutoff criterion for a positive response, a cutoff used by other investigators [Zeiss et al, 1977;Frew et al, 1998;Redlich et al, 2001]. …”