T lymphocyte-mediated protection against Pseudomonas aeruginosa infection in granulocytopenic mice.

Abstract

BALB/c mice immunized with Pseudomonas aeruginosa immunotype 1 polysaccharide develop protective T cell immunity to bacterial challenge. In vitro, T cells from immunized mice kill P. aeruginosa by production of a bactericidal lymphokine. The present study demonstrates that adoptive transfer of T cells from immunized BALB/c mice to granulocytopenic mice resulted in 97% survival on challenge with P. aeruginosa, compared with 17% survival with adoptive transfer of T cells from nonimmune BALB/c mice. This protection is specifically elicited by reexposure to the original immunizing antigen; adoptive recipients cannot withstand challenge with immunotype 3 P. aeruginosa. However, the adoptive recipients do survive simultaneous infection with both P. aeruginosa immunotypes 1 and 3. Adoptive transfer of T cells from the congenic CB.20 mice, which are unable to kill P. aeruginosa in vitro, provides only 20% protection to granulocytopenic mice. These studies indicate that transfer of specific immune T lymphocytes can significantly enhance the resistance to P. aeruginosa infection in granulocytopenic mice.

Cystic fibrosis, gene therapy, and lung inflammation: for better or worse?

“…For example, these authors have shown that adoptive transfer of T cells from mice immunized with P. aeruginosa to granulocytopenic mice resulted in nearly 100% survival upon challenge with P. aeruginosa (12). Similar findings have been reported by others (3).…”

“…Early studies by Markham and coworkers (8,12) suggest that T cell-mediated immune responses are protective against P. aeruginosa infections. For example, these authors have shown that adoptive transfer of T cells from mice immunized with P. aeruginosa to granulocytopenic mice resulted in nearly 100% survival upon challenge with P. aeruginosa (12).…”

Phenotypic and functional characterization of human lymphocytes activated by interleukin-2 to directly inhibit growth of Cryptococcus neoformans in vitro.

“…The data presented herein strongly suggest that IL-2-activated T cells are capable of attacking C. neoformans and directly mediating anticryptococcal activity without MHC restriction. Other investigators have demonstrated that splenic CD8+ T cells, obtained from mice immunized with Pseudomonas aeruginosa polysaccharide antigen, secrete a bactericidal lymphokine (27)(28)(29). The bactericidal effect occurred in the absence of Ab or complement and did not require phagocytic cells.…”

IFN Regulatory Factor 3 Contributes to the Host Response during Pseudomonas aeruginosa Lung Infection in Mice

“…These include neutrophils (1), macrophages (21,22), NKT cells (NKTs) (23), NK cells (24), dendritic cells (25), T cells (26,27), and B cells (28,29). We attempted to examine whether IRF3 regulates immune cell infiltration into the lung in response to P. aeruginosa infection.…”