T-lymphocyte-expressing inflammatory cytokines underlie persistence of proteinuria in children with idiopathic nephrotic syndrome

Guimarães, Fábio Tadeu Lourenço; Melo, Gustavo Eustáquio Brito Alvim de; Cordeiro, Thiago Macedo e; Feracin, Victor; Vieira, Etel Rocha; Pereira, Wagner de Fátima; Pinheiro, Sérgio Veloso Brant; Miranda, Aline Silva de; Silva, Ana Cristina Simões e

doi:10.1016/j.jped.2017.08.005

Cited by 16 publications

(15 citation statements)

References 28 publications

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“…Certain studies have shown that the population of CD8+ T and CD4+ T cells is increased in PNS patients [ 31 , 32 ]. A recent research showed that INS patients had increased expression of TNF- α in CD4-lymphocytes and reduced expression of IFN- γ in CD8-lymphocytes [ 33 ]. B lymphocytes are also involved in the pathogenesis of PNS.…”

Section: Discussionmentioning

confidence: 99%

Renal Tubular Complement 3 Deposition in Children with Primary Nephrotic Syndrome

Wang

Zhu

et al. 2018

BioMed Research International

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Background This study aimed to investigate the clinical significance of complement 3 (C3) deposition in renal tubules of children with primary nephrotic syndrome (PNS). Methods The clinical and pathological characteristics of PNS were retrospectively reviewed in 99 PNS pediatric patients, who were divided into the C3 deposition and the non-C3 deposition groups. Results A total of 39 patients (39.39%) had renal tubule C3 deposition. In the C3 deposition group, the ratios of urine N-acetylglucosaminidase/creatinine (UNAG/Cr), urine β2 microglobulin/creatinine (Uβ2MG/Cr), and urine transferrin/creatinine (UTRF/Cr) were significantly higher than those of the non-C3 deposition group. The patients of the C3 deposition group had lower serum total protein and albumin, higher cholesterol and D-dimer (DD), lower proportion of CD3+CD8+ cells, and higher proportion of CD19+CD23+ cells. The number of the patients with interstitial fibrosis, renal cell vacuolar degeneration, renal tubular immunoglobulin deposition, and severe tubulointerstitial injury in the C3 deposition group was higher than that of the non-C3 deposition group. The C3 deposition intensity was positively correlated with the number of recurrences. Conclusion PNS pediatric patients with C3 deposition in renal tubules have more severe disease condition, tubulointerstitial injury, and recurrence suggesting a worse long-term prognosis.

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Section: Discussionmentioning

confidence: 99%

Renal Tubular Complement 3 Deposition in Children with Primary Nephrotic Syndrome

Wang

Zhu

et al. 2018

BioMed Research International

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“…The current study supports these premises and showed reduced inflammatory markers (IL-6 and IL-8) in children with NS versus healthy children. Similarly, in their cohort of children with NS, Guimarães et al found low levels of systemic inflammatory markers including circulating B lymphocytes, and NK and NKT cells [20].…”

Section: Discussionmentioning

confidence: 78%

Salivary Cytokines in Children with Nephrotic Syndrome versus Healthy Children: A Comparative Study

Polak

Borovitz

Clyman-Levy

et al. 2020

JCM

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Background: The aims of this study were to compare salivary cytokines and total protein between children with nephrotic syndrome (NS) and healthy children, and to examine whether saliva parameters can differentiate between steroid sensitivity and resistance and between disease remission and relapse. Methods: Twenty-seven children with nephrotic syndrome were classified according to steroid sensitivity and resistance, and disease remission and relapse. Twenty healthy children served as controls. Whole saliva samples were collected from all the participants. Urine and blood tests done on the same day as the saliva collection were recorded. Salivary total protein was quantified using bicinchoninic acid and IFNγ, IL-4, IL-8, IL-6, and IL1β levels using ELISA. Results: The mean ages of the nephrotic syndrome and control groups were 11.3 ± 2.4 and 9 ± 4.2, respectively. Compared to the control group, for the nephrotic syndrome group, total salivary protein was significantly lower, as were the levels of all the cytokines examined except IFNγ. Statistically significant differences were not found in any of the salivary markers examined between the children with nephrotic syndrome who were treatment sensitive (n = 19) and resistant (n = 8). Protein and IL-8 salivary levels were lower in the active (n = 7) than in the remission (n = 20) group. Conclusions: Salivary parameters distinguished children with nephrotic syndrome in relapse from healthy children. This may be due to decreased salivary protein excretion, which reflects decreased plasma levels, consequent to proteinuria. Accordingly, salivary markers may be developed as a diagnostic or screening tool for NS activity.

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“…found low levels of systemic in ammatory markers including circulating B lymphocytes, NK and NKT cells [18].…”

Section: Discussionmentioning

confidence: 99%

Salivary cytokines in children with nephrotic syndrome versus healthy children: a comparative study

Pollák

Borovitz

Levy

et al. 2020

Preprint

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Background: The aims of this study were to compare salivary cytokines and total protein, between children with nephrotic syndrome and healthy children; and to examine whether saliva parameters can differentiate between steroid sensitivity and resistance, and between disease remission and relapse. Methods: Twenty-seven children with nephrotic syndrome were classified according to steroid sensitivity and resistance, and disease remission and relapse. Twenty healthy children served as controls. Whole saliva samples were collected from all the participants. Urine and blood tests done on the same day as the saliva collection were recorded. Salivary total protein was quantified using bicinchoninic acid; and IFNγ, IL-4, IL-8, IL-6 and IL1β levels using ELISA. Results: The mean ages of the nephrotic syndrome and control groups were 11.3±2.4 and 9±4.2, respectively. Compared to the control group, for the nephrotic syndrome group, total salivary protein was significantly lower; as were the levels of all the cytokines examined except IFNγ. Statistically significant differences were not found in any of the salivary markers examined, between the children with nephrotic syndrome who were treatment sensitive (n=19) and resistant (n=8). Protein and IL-8 salivary levels were lower in the active (n=7) than in the remission (n=20) group. Conclusions: Salivary parameters distinguished children with nephrotic syndrome in relapse from healthy children. This may be due to decreased salivary protein excretion, which reflects decreased plasma levels, consequent to proteinuria. Accordingly, salivary markers may be developed as a diagnostic or screening tool for NS activity.

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T-lymphocyte-expressing inflammatory cytokines underlie persistence of proteinuria in children with idiopathic nephrotic syndrome

Abstract: Regardless the level of proteinuria, INS patients had increased expression of TNF-α in CD4-lymphocytes and reduced expression of IFN-γ in CD8-lymphocytes. Persistence of proteinuria was associated with higher levels of inflammatory markers.

Cited by 16 publications

References 28 publications

Renal Tubular Complement 3 Deposition in Children with Primary Nephrotic Syndrome

Renal Tubular Complement 3 Deposition in Children with Primary Nephrotic Syndrome

Salivary Cytokines in Children with Nephrotic Syndrome versus Healthy Children: A Comparative Study

Salivary cytokines in children with nephrotic syndrome versus healthy children: a comparative study

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