T-lymphocyte calcium influx characteristics and their modulation by Kv1.3 and IKCa1 channel inhibitors in the neonate

Toldi, Gergely; Treszl, András; Pongor, Vince; Gyarmati, Béla; Tulassay, Tivadar; Vásárhelyi, Barna

doi:10.1093/intimm/dxq063

Cited by 12 publications

(15 citation statements)

References 17 publications

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“…-response, neonatal T-cells present other functional characteristics distinct from T-cells of the adult (11,23). Now we measured reduced baseline cytoplasmic Ca 2+ levels and reduced Ca Indeed we measured lower mitochondrial mass in neonatal CD4-cells than in those from the adult.…”

Section: +mentioning

confidence: 70%

“…We have found that Ca 2+ -response during short-term activation of T-cells is lower in the neonate than in the adult. The immaturity of potassium channels on neonatal cell membrane may be an explanation for this finding (23).…”

Section: +mentioning

confidence: 96%

“…T-cell activation was initiated by phytohemagglutinin (15 mg/ml in final concentration). In each experiment fluorescence emission of sequentially measured cells was monitored for 12 minutes, the time which is needed for the decline of the PHA induced calcium signal in adult and cord blood human T-cells (23). In average, about 1 x 10 6 cells were measured.…”

Section: Cell Activationmentioning

confidence: 99%

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Altered mitochondrial response to activation of T-cells in neonate

Mészáros¹,

Orbán²,

Kaposi³

et al. 2015

Acta Physiologica Hungarica

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Mitochondrial functions have a major impact on T-cell functionality. In this study we characterized whether mitochondrial function in the neonatal T-cells differs from that in the adult T-cells during short T-cell activation. Methods: We used flow cytometry methods to test mitochondrial mass and to monitor mitochondrial Ca 2+ levels, mitochondrial potential and superoxide generation in parallel with cytoplasmic Ca 2+ levels during phythohaemagglutinine-induced activation of CD4+ and CD8+ T-cells of 12 term neonates and 11 healthy adults. Results: Baseline mitochondrial mass of CD4+ and CD8+ cells was lower in the neonate than in the adult. In comparison with the adult, neonatal resting CD4+ T-cells had lower cytoplasmic Ca 2+ levels and this was associated with normal activation induced Ca 2+ -response. During short-term activation cytoplasmic Ca 2+-response was lower in neonatal than in adult CD8+ T-cells. Mitochondrial Ca 2+ uptake was increased in CD4+ neonatal T cells while it decreased in CD8+ T-cells. Mitochondrial depolarization was increased in CD4+ and decreased in CD8+ neonatal T-cells compared to adults. Superoxide generation was higher and equal in neonatal CD4+ and CD8+ cells, respectively, compared to the adult ones. Conclusion: Our data suggest that neonatal T-cells exhibit marked differences in mitochondrial function and superoxide generation compared to adult T-cells.

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Section: +mentioning

confidence: 70%

Section: +mentioning

confidence: 96%

Section: Cell Activationmentioning

confidence: 99%

See 1 more Smart Citation

Altered mitochondrial response to activation of T-cells in neonate

Mészáros¹,

Orbán²,

Kaposi³

et al. 2015

Acta Physiologica Hungarica

View full text Add to dashboard Cite

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“…Note that these calculated parameters differ from the parameters that would be recorded during the measurement for each cell: from a single measurement one can only derive a single value for each such calculated parameter. Jakubczak et al;2006, Demkow et al;2009, Toldi et al;2010b, Szalay et al, 2012), t-test (used in Lund-Johansen et al, 1992, paired Wilcoxon-test (used in Schepers et al, 2009;Toldi et al, 2010a, Szalay et al, 2012.…”

Section: Smoothing Methodsmentioning

confidence: 99%

“…We aimed to characterize the calcium influx kinetics upon activation in major T lymphocyte subsets (i.e. Th1, Th2, CD4 and CD8 cells) in the neonate, and its sensitivity to the specific inhibition of Kv1.3 and IKCa1 lymphocyte potassium channels (Toldi et al, 2010b (Adkins, 1999;Cohen et al, 1999). The functional impairment of lymphocyte potassium channels may be of importance in those mechanisms.…”

Section: Lymphocyte Activation and Potassium Channels In The Newbornmentioning

confidence: 99%

Experimental Conditions and Mathematical Analysis of Kinetic Measurements Using Flow Cytometry – The FacsKin Method

Kaposi¹,

Toldi²,

Meszaros³

et al. 2012

Flow Cytometry - Recent Perspectives

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Prevalence of Intracellular Galectin-1-Expressing Lymphocytes in Umbilical Cord Blood in Comparison with Adult Peripheral Blood

Kollár

Sándor

Molvarec

et al. 2012

Biology of Blood and Marrow Transplantation

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Umbilical cord blood (UCB) is a promising alternative for the treatment of hematological malignancies. The lower immune reactivity of UCB lymphocytes is a well-known phenomenon; however, immune tolerance mechanisms are not fully elucidated. Galectin-1 has strong immunosuppressive properties and plays a key role in the regulation of immune reactivity. We aimed to determine the properties of intracellular galectin-1 (Gal-1)-producing cells within CD3, CD4, CD8, regulatory T (Treg), and natural killer (NK) cells in UCB compared to adult peripheral blood (APB). We took peripheral blood samples from 22 healthy adults and cord blood samples from 19 healthy, term neonates. Intracellular Gal-1 expression was determined by flow cytometry in the above subsets. Furthermore, we assessed the prevalence of naive and memory T cells that play a role in the regulation of immune reactivity. We also performed functional analyses to assess the effect of exogenous Gal-1 on the rate of proliferation of T lymphocytes isolated from APB and UCB. The prevalence of intracellular Gal-1-expressing CD3, CD4, CD8, Treg and NK lymphocytes was lower in UCB than in APB. However, their capability to produce Gal-1 reaches the level seen in adults. The prevalence of naive cells was higher, whereas that of central and effector memory T cells was lower in UCB compared with APB. Lower Gal-1-producing cell proportion might be due to the naivety of neonatal lymphocytes, as indicated by the positive correlation detected between the number of CD3 lymphocytes expressing intracellular Gal-1 and the prevalence of memory T cells. The intracellular expression of Gal-1 may be down-regulated in neonatal lymphocytes due to the already reduced immune reactivity of UCB. In contrast with previous findings, our results indicate that the administration of exogenous Gal-1 failed to decrease the rate of proliferation in T lymphocytes isolated from either APB or UCB. This suggests that Gal-1-expressing lymphocytes are unlikely to play a major role in mitigating the immune reactivity of UCB.

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T-lymphocyte calcium influx characteristics and their modulation by Kv1.3 and IKCa1 channel inhibitors in the neonate

Cited by 12 publications

References 17 publications

Altered mitochondrial response to activation of T-cells in neonate

Altered mitochondrial response to activation of T-cells in neonate

Experimental Conditions and Mathematical Analysis of Kinetic Measurements Using Flow Cytometry – The FacsKin Method

Prevalence of Intracellular Galectin-1-Expressing Lymphocytes in Umbilical Cord Blood in Comparison with Adult Peripheral Blood

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