T follicular helper and T follicular regulatory cells in colorectal cancer: A complex interplay

Hetta, Helal F; Elkady, Azza; Yahia, Ramadan; Meshall, Ahmed Kh.; Saad, Mahmoud; Mekky, Mohamed A.; Al-Kadmy, Israa M.S.

doi:10.1016/j.jim.2020.112753

Cited by 30 publications

(28 citation statements)

References 44 publications

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“…In the past decade, nanoparticle (NP)-based approaches have emerged for the diagnosis and treatment of cancer [1,2]. Colon cancer is one of the most common gastrointestinal malignancy-related causes of death worldwide, with very few or no treatment options [3]. Recently herbal medicine has shown a new hope to fight against colon cancer.…”

Section: Introductionmentioning

confidence: 99%

Pleurotus sajor-caju-Mediated Synthesis of Silver and Gold Nanoparticles Active against Colon Cancer Cell Lines: A New Era of Herbonanoceutics

et al. 2020

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Herbal medicines are widely used worldwide and much appreciated because of their fewer side effects and the ability to fight diseases at the root cause. Active ‘phyto’ ingredients require a scientific approach and a mechanism to distribute components at the target site for better therapeutic results. Nanotechnology, on the other hand, has created new hope for cancer treatment but is still far from being proven in clinical settings. This article combines a unique approach to synthesis with the use of Pleurotus sajor-caju, followed by microwave irritation of silver and gold nanoparticles that ensures the capping of the active phyto ingredient and further enhances the effects of nanomedicine to fight colon cancer, thus opening a new era of what we call herbonanoceutics. The article also compares the characteristics and properties of silver (Au) and gold (Ag) nanoparticles synthesized by an in house developed novel microwave-assisted rapid green synthesis method. The as-prepared Ag NPs and Au NPs were compared using ultraviolet-visible spectroscopy (UV-Vis), Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), and energy dispersive spectroscopy (EDS). Our comparative study revealed that both assemblies display face-centred cubic structures (FCCs) and are nanocrystalline in nature. The advantage of the approach was that the sizes of gold and silver were identical in range with a similar distribution pattern. This has helped us to study the activity against colon cancer cell line (HCT-116) without incoherence since size plays a key role in the application. More specifically, morphological changes, cell viability, the production of reactive oxygen species (ROS) and the fragmentation of DNA have been further reported to assess better the results obtained with the two metals. Our results suggest that the newly adopted synthesis method may ensure the dual benefits from phyto ingredients which further enhances the effectiveness of advanced nanomedicine.

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Section: Introductionmentioning

confidence: 99%

Pleurotus sajor-caju-Mediated Synthesis of Silver and Gold Nanoparticles Active against Colon Cancer Cell Lines: A New Era of Herbonanoceutics

et al. 2020

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“…Accumulating evidence has suggested that dysregulation of follicular helper T cells is involved in various diseases. Numerous studies have demonstrated that the infiltration of follicular helper T cells in multiple malignancies is positively associated with survival, including that of non-small-cell lung cancer [ 36 ], colorectal cancer [ 37 ], and breast cancer [ 38 ]. Furthermore, Bronsert et al reported that high numbers and densities of PD1+ follicular helper T cells were linked to adverse prognosis in triple-negative breast cancer [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

Identification of Novel Prognostic Signatures for Clear Cell Renal Cell Carcinoma Based on ceRNA Network Construction and Immune Infiltration Analysis

Zhou

Wen

et al. 2022

Disease Markers

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Objective. Clear cell renal cell carcinoma (ccRCC) carries significant morbidity and mortality globally and is often resistant to conventional radiotherapy and chemotherapy. Immune checkpoint blockade (ICB) has received attention in ccRCC patients as a promising anticancer treatment. Furthermore, competitive endogenous RNA (ceRNA) networks are crucial for the occurrence and progression of various tumors. This study was aimed at identifying reliable prognostic signatures and exploring potential mechanisms between ceRNA regulation and immune cell infiltration in ccRCC patients. Methods and Results. Gene expression profiling and clinical information of ccRCC samples were obtained from The Cancer Genome Atlas (TCGA) database. Through comprehensive bioinformatic analyses, differentially expressed mRNAs (DEmRNAs; n = 131 ), lncRNAs (DElncRNAs; n = 12 ), and miRNAs (DEmiRNAs; n = 25 ) were identified to establish ceRNA networks. The CIBERSORT algorithm was applied to calculate the proportion of 22 types of tumor-infiltrating immune cells (TIICs) in ccRCC tissues. Subsequently, univariate Cox, Lasso, and multivariate Cox regression analyses were employed to construct ceRNA-related and TIIC-related prognostic signatures. In addition, we explored the relationship between the crucial genes and TIICs via coexpression analysis, which revealed that the interactions between MALAT1, miR-1271-5p, KIAA1324, and follicular helper T cells might be closely correlated with the progression of ccRCC. Ultimately, we preliminarily validated that the potential MALAT1/miR-1271-5p/KIAA1324 axis was consistent with the ceRNA theory by qRT-PCR in the ccRCC cell lines. Conclusion. On the basis of the ceRNA networks and TIICs, we constructed two prognostic signatures with excellent predictive value and explored possible molecular regulatory mechanisms, which might contribute to the improvement of prognosis and individualized treatment for ccRCC patients.

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“…In our research, by CIBERSORT analysis, we found that there were no statistically difference in the percentages of bone marrow T cells subtypes except follicular helper (Tfh) cells between PAX5 mutations B-ALL and wild type B-ALL. The role of Tfh cells was not explicit, some papers showed that tumor in ltrating Tfh cells were related to good clinical prognosis for breast cancer [30,31]. The proportion of CD8 + T cells,as an important effector cells in the tumor environment, had no signi cant difference between PAX5 mutations B-ALL and wild type B-ALL.…”

Section: Discussionmentioning

confidence: 99%

Difference in leukemia microenvironment induced by PAX5 mutations in acute lymphoblastic leukemia

Wei

Gong

Wang

et al. 2022

Preprint

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Background:PAX5 mutations are involved in the development and recurrence of leukemia. There were few studies on how PAX5 mutations influencing leukemia bone marrow microenvironment. Methods: We obtained the mRNA profiling of B-acute lymphoblastic leukemia(ALL) patients from GSE11877 and the clinical features (including molecular genetics) from the literature. We identified differentially expressed genes (DEGs) between B-ALL with PAX5 mutations and B-ALL without PAX5 mutations in leukemia microenvironment using the LIMMA package of R. We assessed immune cell types by CIBERSORT. DEGs were analyzed using the DAVID,STRING and Coexpedia. Results:The results from CIBERSORT analysis showed that M0 macrophages were significantly increased from ALL PAX5 mutations. The results from DAVID analysis indicated that these DEGs were mostly involved in phosphorylation and antigen presentation from biological process categories. These DEGs may be involved in changes in macrophages. Other analyses from PPI network and co -expression network found three common genes such as IL1B, CD34, PDGFRB, which were highly related to angiogenesis. Conclusions:These changes hinted that PAX5 mutations led to macrophage changes, and macrophage changes were related to processes of antigen presentation and angiogenesis in leukemia microenvironment.

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T follicular helper and T follicular regulatory cells in colorectal cancer: A complex interplay

Cited by 30 publications

References 44 publications

Pleurotus sajor-caju-Mediated Synthesis of Silver and Gold Nanoparticles Active against Colon Cancer Cell Lines: A New Era of Herbonanoceutics

Pleurotus sajor-caju-Mediated Synthesis of Silver and Gold Nanoparticles Active against Colon Cancer Cell Lines: A New Era of Herbonanoceutics

Identification of Novel Prognostic Signatures for Clear Cell Renal Cell Carcinoma Based on ceRNA Network Construction and Immune Infiltration Analysis

Difference in leukemia microenvironment induced by PAX5 mutations in acute lymphoblastic leukemia

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