T-DNA organization in homogeneous and heterogeneous octopine-type crown gall tissues of nicotiana tabacum

Ooms, G.; Bakker, A.Z.; Molendijk, Lucy; Wullems, G. J.; Gordon, Milton P.; Nester, Eugene W.; Schilperoort, R. A.

doi:10.1016/0092-8674(82)90255-0

Cited by 82 publications

(48 citation statements)

References 37 publications

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“…the left border was 'skipped'). There is a precedence for such 'border skipping' events: Ooms et aL (1982) described a crown gall tumor that contained Ti-plasmid sequences extending beyond the left T-DNA border. Ursic et aL (1983) similarly described a crown gall tumor that contained DNA sequences beyond the bounds of commonly defined T-DNA left and right borders.…”

Section: Identification Of T-dna and Binary Vector 'Backbone' Single-mentioning

confidence: 99%

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Integration of T‐DNA binary vector ‘backbone’ sequences into the tobacco genome: evidence for multiple complex patterns of integration

Kononov

Bassüner

Gelvin³

1997

The Plant Journal

220

139

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Section: Identification Of T-dna and Binary Vector 'Backbone' Single-mentioning

confidence: 99%

“…Indeed, T-DNA is defined by many scientists as those sequences that tie between these borders. However, evidence in the literature suggests that DNA sequences residing outside the T-DNA borders may occasionally transfer to the plant (Ooms et aL, 1982). Recently, Martineau et aL (1994) reported that binary vector 'backbone' sequences (i.e.…”

mentioning

confidence: 99%

Integration of T‐DNA binary vector ‘backbone’ sequences into the tobacco genome: evidence for multiple complex patterns of integration

Kononov

Bassüner

Gelvin³

1997

The Plant Journal

220

139

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“…tabacum cv Xanthi plants were transformed with Agrobacterium tumefaciens LBA 4404 (Ooms and Bakker, 1982), by cocultivation as described (Thornburg et al, 1987). Transgenic progeny lines were selected on MS plates containing 0.1 mg/mL naphthalene acetic acid, 1.0 mg/mL benzylaminopurine, 50 mg/mL Hygromycin B, and 100 mg/mL Carbenicillin.…”

Section: Plant Transformationmentioning

confidence: 99%

The MYB305 Transcription Factor Regulates Expression of Nectarin Genes in the Ornamental Tobacco Floral Nectary

et al. 2009

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We have isolated and characterized the cDNA encoding the ornamental tobacco (Nicotiana langsdorffii X N. sanderae) homolog of the antirrhinum (Antirrhinum majus) MYB305. This transcription factor was robustly expressed at Stage 12 of nectary development but was only weakly expressed in the earlier Stage 6 nectaries. The ornamental tobacco MYB305 contains a conserved R2R3 MYB DNA binding domain with 76 amino acids in the activation domain. A green fluorescent protein-MYB305 fusion localized to nucleus of tobacco protoplasts and yeast one-hybrid assays demonstrated that it functions as a transcription activator. A conserved 23-amino acid C-terminal domain is required to activate gene expression. The coding region of the myb305 cDNA was expressed in Escherichia coli as a glutathione S-transferase fusion protein and was purified to homogeneity. This protein shows binding to two consensus MYB binding sites on the ornamental tobacco Nectarin I (nec1) promoter as well as to the single site located on the Nectarin V (nec5) promoter. Deletions of either of the binding sites from the nec1 promoter significantly reduced expression in nectary tissues. Temporally, MYB305 expression precedes that of nec1 and nec5, as would be expected if the MYB305 factor regulates expression of the nec1 and nec5 genes. Ectopic expression of MYB305 in foliage was able to induce expression of both nec1 and nec5, as well as two flavonoid biosynthetic genes in the foliage. Finally, RNA interference knockdown of MYB305 resulted in reduced expression of both nectarins and flavonoid biosynthetic genes. We conclude that expression of MYB305 regulates expression of the major nectarin genes in the floral nectary.

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“…We performed nanopore sequencing on each line using a single R9.4 flow cell (Table 1 and Supplementary Table 1) and assembled each genome using minimap/miniasm followed by three rounds of racon [25] and one round of Pilon [26]. We assembled the three lines into 40 contigs (Col-0; longest 16 Table 2). The remaining short contigs (< 50 kb) encode only highly repetitive sequences such as rDNA and centromeric repeats that cannot be placed onto the reference.…”

Section: Assembly Of Highly Contiguous Genomes From Minion Datamentioning

confidence: 99%

“…While the exact mechanisms behind this error prone integration are poorly understood, it is known that insertion events generally occur at multiple locations throughout the genome [5,10]. T-DNA insertions also frequently contain the vector backbone, and occur as direct or inverted repeats of the T-DNA, resulting in large intra-and inter-chromosomal rearrangements [6,11,12,13,14,15,16,17,18,19,20].…”

Section: Introductionmentioning

confidence: 99%

The complex architecture of plant transgene insertions

Jupe

et al. 2018

Preprint

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T-DNA organization in homogeneous and heterogeneous octopine-type crown gall tissues of nicotiana tabacum

Cited by 82 publications

References 37 publications

Integration of T‐DNA binary vector ‘backbone’ sequences into the tobacco genome: evidence for multiple complex patterns of integration

Integration of T‐DNA binary vector ‘backbone’ sequences into the tobacco genome: evidence for multiple complex patterns of integration

The MYB305 Transcription Factor Regulates Expression of Nectarin Genes in the Ornamental Tobacco Floral Nectary

The complex architecture of plant transgene insertions

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