T cells, dendritic cells and epithelial cells in intestinal homeostasis

Westendorf, Astrid M.; Fleissner, Diana; Hansen, Wiebke; Buer, Jan

doi:10.1016/j.ijmm.2009.08.009

Cited by 39 publications

(36 citation statements)

References 101 publications

(103 reference statements)

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“…These cells are able to discriminate between an assortment of antigens using pathogen recognition receptors (PRRs), such as Toll-like receptors (TLRs) (2,9).…”

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confidence: 99%

Multistrain Probiotic Modulation of Intestinal Epithelial Cells' Immune Response to a Double-Stranded RNA Ligand, Poly(I·C)

MacPherson

Audy

Mathieu

et al. 2014

Appl Environ Microbiol

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A commercially available product containing three probiotic bacterial strains (Lactobacillus helveticus R0052, Bifidobacterium longum subsp. infantis R0033, and Bifidobacterium bifidum R0071) was previously shown in animal trials to modulate both T H 1 and T H 2 immune responses. Clinical studies on this combination of bacteria have also shown positive health effects against seasonal winter diseases and rotavirus infection. The goal of this study was to use a well-established in vitro intestinal epithelial (HT-29) cell model that has been shown to constitutively express double-stranded RNA (dsRNA) sensors (Toll-like receptor 3 [TLR3], retinoic acid-inducible gene I, melanoma differentiation-associated gene 5, and dsRNA-activated protein kinase). By using the HT-29 cell model, we wanted to evaluate whether or not this combination of three bacteria had the capacity to immune modulate the host cell response to a dsRNA ligand, poly(I·C). Using a custom-designed, two-color expression microarray targeting genes of the human immune system, we investigated the response of HT-29 cells challenged with poly(I·C) both in the presence and in the absence of the three probiotic bacteria. We observed that the combination of the three bacteria had a major impact on attenuating the expression of genes connected to proinflammatory T H 1 and antiviral innate immune responses compared to that obtained by the poly(I·C)-only challenge. Major pathways through which the multistrain combination may be eliciting its immune-modulatory effect include the TLR3 domain-containing adapter-inducing beta interferon (TRIF), mitogenactivated protein kinase, and NF-B signaling pathways. Such a model may be useful for selecting potential biomarkers for the design of future clinical trials.

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“…These cells are able to discriminate between an assortment of antigens using pathogen recognition receptors (PRRs), such as Toll-like receptors (TLRs) (2,9).…”

mentioning

confidence: 99%

Multistrain Probiotic Modulation of Intestinal Epithelial Cells' Immune Response to a Double-Stranded RNA Ligand, Poly(I·C)

MacPherson

Audy

Mathieu

et al. 2014

Appl Environ Microbiol

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show abstract

“…A continuous and sensitive balance thus occurs between differentiation into regulatory T-cell subsets and effector T cells. The functional requirements to fight against different pathogens led to the identification of distinct CD4 þ and CD8 þ T-cell populations dealing with active defence and characterized by specific cytokine profiles [2].…”

Section: Introductionmentioning

confidence: 99%

Intestinal T cells of Dicentrarchus labrax (L.): Gene expression and functional studies

Picchietti

Guerra

Bertoni

et al. 2011

Fish & Shellfish Immunology

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Cellular and molecular data have evidenced a gut-associated lymphoid tissue in a variety of teleost species, abundantly containing T cells, whose origin, selection and functions are still unclear. This study reports CD4, CD8-α, MHCI-α, MHCII-β, rag-1 and TCR-β gene transcription along the intestine (anterior, middle and posterior segments) and in the thymus of one year-old Dicentrarchus labrax (L.). Real-time PCR findings depicted a main role of the thymus in T-cell development, but also rag-1 and CD8-α transcripts are detected in the intestine, having significant expression in the posterior segment. In the whole intestine TCR-β and CD8-α exceeded CD4 transcripts. RNA ISH confirmed these data and detailed that mucosal CD8-α+ cells were especially numerous in the epithelium and in aggregates in the lamina propria. Regional differences in T-cell-specific gene expressions are first described in the intestine of a bony fish. High non-specific cytotoxic activity against xenogeneic and allogeneic cells was found in lymphocytes purified from the intestinal mucosa, providing further insight into their local defence roles.

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“…Under steady-state conditions, IECs create a tolerogenic environment; however, in the presence of pathogenic microorganisms, they secrete cytokines that are crucial for the recruitment and activation of inflammatory cells. These two functions of IECs-to distinguish between the diverse elements of the intestinal flora and to respond to invading pathogens-are principally determined by pattern recognition receptors (PRRs) (45). Toll-like receptors (TLRs) are an important class of PRRs in innate immunity, and TLRs play a critical role in pathogen recognition and host defense.…”

mentioning

confidence: 99%

Immunobiotic Lactobacillus jensenii Elicits Anti-Inflammatory Activity in Porcine Intestinal Epithelial Cells by Modulating Negative Regulators of the Toll-Like Receptor Signaling Pathway

et al. 2012

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T cells, dendritic cells and epithelial cells in intestinal homeostasis

Cited by 39 publications

References 101 publications

Multistrain Probiotic Modulation of Intestinal Epithelial Cells' Immune Response to a Double-Stranded RNA Ligand, Poly(I·C)

Multistrain Probiotic Modulation of Intestinal Epithelial Cells' Immune Response to a Double-Stranded RNA Ligand, Poly(I·C)

Intestinal T cells of Dicentrarchus labrax (L.): Gene expression and functional studies

Immunobiotic Lactobacillus jensenii Elicits Anti-Inflammatory Activity in Porcine Intestinal Epithelial Cells by Modulating Negative Regulators of the Toll-Like Receptor Signaling Pathway

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