T Cell V‐Gene Usage in Man in Some Normal and Abnormal Situations<sup>a</sup>

Wigzell, Hans; Grünewald, Johan; Tehrani, Mahmood Jeddi; Esin, Semih; Mellstedt, H; Österborg, Anders; DerSimonian, Harout; Brenner, Michael B.; Åhlberg, R.; Lefvert, Ann‐Kari; Orn, M. H; Eklund, Aron Charles; Ch, Janson

doi:10.1111/j.1749-6632.1991.tb33433.x

Cited by 5 publications

(4 citation statements)

References 21 publications

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“…Recently, molecular probes and MoAbs to the variable (V) genes ofthe TCR complex and their products have been used to study the repertoire of T cell subsets in autoimmune and chronic inflammatory diseases [11,12]. Changes in the number of T cells expressing restricted TCR V gene products suggest that superantigens may be involved in the disease pathogenesis in such patients [13,14].…”

Section: Introductionmentioning

confidence: 99%

Cytotoxic activity of Vβ8+ T cells in Crohn's disease: the role of bacterial superantigens

Baca-Estrada

Wong

Croitoru

1995

Clinical and Experimental Immunology

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SUMMARYIn Crohn's disease, disease-related stimuli could alter the T cell receptor (TCR) repertoire. To examine the possibility that changes in function may occur in T cell subsets without obvious changes in expression of TCR, we analysed the TCR repertoire of cytotoxic T lymphocytes in Crohn's disease peripheral blood. Furthermore, we examined the effect of bacterial superantigens, staphylococcal enterotoxin B (SEB) and E (SEE) on the cytotoxic function of T cell subsets bearing different TCR V genes using MoAbs specific for CD3 and TCR V gene products in a redirected cytotoxicity assay. There was no difference between patients and controls in the cytotoxicity measured in concanavalin A (Con A)-stimuiated peripheral blood mononuclear celts (PBMC) with anti-CD3 or with six of seven anti-TCR V gene MoAbs. However, the cytotoxicity of \3^ T cells was decreased in Crohn's disease patients. This was not due to a decrease in total or CD8 ^ T cells expressing V/38. Furthermore, in normal subjects, PBMC stimulation with SEE and SEB selectively expanded and increased the cytotoxicity of V/?8 and V/312 T cells, respectively. In Crohn's disease, although SEB stimulation increased the number and cytolytic function of the V/312 subset, SEE stimulation failed to increase cytolytic activity of V/38^ T cells in spile of the expansion of V/38^ Tceils. These results suggest that the changes in cytotoxic function observed in V/38 T cells in Crohn's patients may reflect previous exposure to a V/58-seIective superantigen.

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Section: Introductionmentioning

confidence: 99%

Cytotoxic activity of Vβ8+ T cells in Crohn's disease: the role of bacterial superantigens

Baca-Estrada

Wong

Croitoru

1995

Clinical and Experimental Immunology

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“…Collectively, these observations suggest that sarcoid CD4 cells are not randomly activated, but rather that they proliferate in response to a specific unknown antigenic pressure, that favours the cell proliferation of a limited number of clones. Because there are no indications of HLA sharing between sarcoid patients, these findings suggest the possibility that an entity behaving as a "superantigen" may be linked to the development of the sarcoid granulomatous damage [116]. Alternatively, it is possible that the preferential expression of particular V region segments may be due to the accumulation of T-effector cells that acquire a peculiar tropism for the pulmonary tract, in that they express certain V genes of the TCR.…”

Section: Pulmonary T-cells In Patients With Interstitjal Lung Diseasesmentioning

confidence: 97%

“…Different groups have recently investigated the genomic configuration of the TCR in sarcoidosis and HP [112][113][114][115][116]. A marked quantitative bias in the usage of P-chain constant region segments (C~1 versus C~2) and variable elements (VJ38, VJ314, Va2,3) by T-cells was demonstrated in sarcoid lung.…”

Section: Pulmonary T-cells In Patients With Interstitjal Lung Diseasesmentioning

confidence: 99%

Pulmonary immune cells in health and disease: lymphocytes

Agostini¹,

Chilosi²,

Zambello³

et al. 1993

Eur Respir J

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Immunological defence mechanisms of the lung are provided by several different types of immunocompetent cell. Among these, the most important are lymphocytes, alveolar macrophages and neutrophils. This review focuses mainly on the pattern of physiological activities of pulmonary lymphocytes in the local host defences. The article provides an overview of the events leading to the recruitment, homing and activity of lymphocytes in the lower respiratory tract. The pathways through which the pulmonary lymphoid system recognizes, destroys and contributes to removing potentially harmful inhaled antigenic materials are also briefly discussed. The final section of the article highlights the pathogenetic role envisaged for lymphoid cells in disease states, in the context of the known biology of these cells.

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“…With the availability of molecular probes and monoclonal antibodies to TCR, changes in TCR expression have been examined to try to identify specific antigenic stimuli. This examination includes the use of monoclonal antibodies to TCR, variable-gene products and the use of reverse transcriptase-polymerase chain reaction analysis for variable-gene mRNA in chronic inflammatory and autoimmune diseases (40)(41)(42). Early studies in IBD failed to show clonal rearrangements at either the a/b or g TcR loci in LPL (43).…”

Section: Alterations Of Mucosal T Lymphocytes In Ibdmentioning

confidence: 99%

T Cell Repertoire and Inflammatory Bowel Disease

Croitoru

Wong

Baca-Estrada

1996

Canadian Journal of Gastroenterology

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The diversity of the T cell receptor repertoire is generated through rearrangement of the variable, junctional and constant region genes. Selection processes in the thymus and periphery serve to eliminate self-reacting T cells, thereby preventing autoimmune disease. The possibility that inflammatory bowel disease (IBD) is an autoimmune disease has led to the search for an auto-antigen. In addition, studies are exploring the T cell receptor repertoire in IBD patients for changes that may provide clues regarding etiopathogenesis. Using monoclonal antibodies to T cell receptor variable-gene products or polymerase chain reaction analysis of variable-gene mRNA expression, the mucosal T cell repertoire has been examined in humans. The intestinal intraepithelial lymphocytes show a significant degree of oligoclonal expansion that may represent local antigen exposure or unique selection processes. This is in keeping with studies that show that murine intestinal intraepithelial lymphocytes undergo positive and possibly negative selection independent of the thymus. In the inflamed human gut, shifts in the T cell receptor repertoire may also reflect recruitment of peripheral T cells to the gut. In one study, a subset of Crohn’s disease patients was shown to have an increase in the proportion of variable β8 peripheral blood lymphocyte and mesenteric lymph node cells, suggesting a superantigen effect. The authors hypothesized that changes in the functional T cell receptor repertoire can also occur which might be independent of changes in the distribution of T cells expressing variable β T cell receptors. In fact, the authors have shown there is a selective decrease in the cytotoxic function of peripheral variable β8 T cells in Crohn’s disease. Furthermore, stimulation with the variable β8 selective bacterial enterotoxin staphylococcal enterotoxin E failed to increase the cytotoxic function in this subset of Crohn’s disease patients compared with controls. This suggests that in Crohn’s disease, variable β8 T cells have undergone an alteration in function that may reflect previous exposure to a superantigen-like stimulus. The relationship to the etiology and pathogenesis of IBD remains to be defined.

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T Cell V‐Gene Usage in Man in Some Normal and Abnormal Situations^a

Cited by 5 publications

References 21 publications

Cytotoxic activity of Vβ8+ T cells in Crohn's disease: the role of bacterial superantigens

Cytotoxic activity of Vβ8+ T cells in Crohn's disease: the role of bacterial superantigens

Pulmonary immune cells in health and disease: lymphocytes

T Cell Repertoire and Inflammatory Bowel Disease

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T Cell V‐Gene Usage in Man in Some Normal and Abnormal Situationsa

Cited by 5 publications

References 21 publications

Cytotoxic activity of Vβ8+ T cells in Crohn's disease: the role of bacterial superantigens

Cytotoxic activity of Vβ8+ T cells in Crohn's disease: the role of bacterial superantigens

Pulmonary immune cells in health and disease: lymphocytes

T Cell Repertoire and Inflammatory Bowel Disease

Contact Info

Product

Resources

About

T Cell V‐Gene Usage in Man in Some Normal and Abnormal Situations^a