T cell Subsets in Peripheral Blood of Women with Recurrent Implantation Failure

Ghaebi, Mahnaz; Abdolmohammadi-Vahid, Samaneh; Ahmadi, Majid; Eghbal‐Fard, Shadi; Dolati, Sanam; Nouri, Mohammad; Talebi, Mehdi; Hamdi, Kobra; Marofi, Faroogh; Aghebati‐Maleki, Leili; Jadidi‐Niaragh, Farhad; Dopour, Masoume; Yousefi, Mehdi

doi:10.1016/j.jri.2018.11.002

Cited by 54 publications

(32 citation statements)

References 40 publications

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“…MiR-155 can regulate Th17/T reg cell balance by inhibiting cytotoxic T-lymphocyte-associated protein 4 expressions, and the overexpression of miR-155 reduces follicular T reg cells, while central T reg cells and miR-155-5p further target sirtuin-1 to maintain the balance of Th17/T reg cells [ 80 ]. Furthermore, miR-122a, miR-146a, miR-155, and miR-181a are highly expressed in T cells, with a close connection with T reg cells, which can target interferon regulatory factor 4 for suppressing IL-6 secretion and inducing IL-17A production, thus assisting in the mitigation of IBD [ 81 , 82 , 83 , 84 ]. In addition, the overexpression of miR-125a can inhibit the differentiation of CD4 + T cells into either Th1 or Th17 cells, which also contributes to immune-regulation [ 85 ].…”

Section: Microrna In Regulating the Intestinal Immune Systemmentioning

confidence: 99%

MicroRNAs Regulate Intestinal Immunity and Gut Microbiota for Gastrointestinal Health: A Comprehensive Review

Zhang

Chen

et al. 2020

Genes

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MicroRNAs are small non-coding RNAs regulating gene expression at the post-transcriptional level. The regulation of microRNA expression in the gut intestine is gradually recognized as one of the crucial contributors of intestinal homeostasis and overall health. Recent studies indicated that both the microRNAs endogenous in the gut intestine and exogenous from diets could play influential roles in modulating microbial colonization and intestinal immunity. In this review, we discuss the biological functions of microRNAs in regulating intestinal homeostasis by modulating intestinal immune responses and gut microbiota. We particularly focus on addressing the microRNA-dependent communication and interactions among microRNA, gut microbiota, and intestinal immune system. Besides, we also summarize the roles of diet-derived microRNAs in host-microbiome homeostasis and their benefits on intestinal health. A better understanding of the relationships among intestinal disorders, microRNAs, and other factors influencing intestinal health can facilitate the application of microRNA-based therapeutics for gastrointestinal diseases.

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Section: Microrna In Regulating the Intestinal Immune Systemmentioning

confidence: 99%

MicroRNAs Regulate Intestinal Immunity and Gut Microbiota for Gastrointestinal Health: A Comprehensive Review

Zhang

Chen

et al. 2020

Genes

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“…During the secretory phase, circulating Treg proportions were significantly increased in infertile compared to fertile women. Circulating and endometrial/decidual Tregs have been well shown to be reduced in women with recurrent pregnancy loss and recurrent implantation failure [30,[33][34][35][36]. Tregs regulate maternal immune tolerance to implantation and continuing pregnancy, and while it is clear that endometriosis is associated with reduced fertility, the mechanisms remain largely unclear [27,28].…”

Section: Discussionmentioning

confidence: 99%

“…Specific immune cell populations, such as Tregs, are critical in the regulation of maternal immune tolerance of the implanting embryo and developing fetus [31,32]. Furthermore, circulating and endometrial/decidual Tregs are reduced in women with recurrent pregnancy loss and recurrent implantation failure [30,[33][34][35][36].…”

Section: Introductionmentioning

confidence: 99%

Circulating and Endometrial Regulatory T Cell and Related Populations in Endometriosis and Infertility: Endometriosis Is Associated with Blunting of Endometrial Cyclical Effects and Reduced Proportions in Moderate-Severe Disease

et al. 2021

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Evidence to date supports regulatory T cell (Treg) alterations in endometriosis; however, the relationship remains unclear, and Tregs have not previously been investigated with respect to infertility in endometriosis. This prospective cross-sectional cohort study details circulating and endometrial tissue-specific disturbances in Tregs and broader gated populations in women of reproductive age with and without endometriosis (n = 57 and 29, respectively) using flow cytometry and immunohistochemistry. Participants were characterised by menstrual cycle phase, r-ASRM endometriosis disease stage and fertility status.In the endometrium of women with endometriosis, endometrial Tregs and CD4+ lymphocyte proportions did not change between the proliferative and secretory phases, while in women without the disease, they significantly decreased (p = 0.045 and p = 0.039, respectively). In women with endometriosis, endometrial Tregs were lower than in women without endometriosis overall (p = 0.050 as a proportion of all CD45+ immune cells). We have shown for the first time that proportions of CD4+ lymphocytes (p = 0.021), overall lymphocytes (p = 0.034) and non-granulocytes (p = 0.027) were significantly decreased in the endometrium of women with moderate-severe (r-ASRM stages III and IV) compared to minimal-mild (r-ASRM stages I and II) endometriosis. During the secretory phase, circulating Treg proportions were significantly increased in infertile compared to fertile women (p = 0.049). This study confirms differences in endometrial Tregs in women with endometriosis, with blunting of normal menstrual cyclical variations, reduced proportions during the proliferative phase and disease stage-specific relationships.

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“…High level expression of GITR after activation of CD8 + Tregs is essential for costimulation of CD8 + T cells, because the lack of GITR will reduce the proliferation response of these cells to costimulation of CD28 [21]. The expression of GITR was decreased in repeated implantation failure (RIF) or unexplained RPL [55,56], which indicates that GITR may play critical roles in regulating the immune response during pregnancy.…”

Section: Discussionmentioning

confidence: 99%

The Fluctuation of Regulatory T Cells during Pregnancy and Obstetrical Complications

Zhao

Bao

et al. 2020

Preprint

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Background: Regulatory T cells (Tregs) are critical immunomodulators during pregnancy by preventing maternal T-cell activation against fetal cells. However, how characteristics of maternal Tregs vary during pregnancy is still unclear. We analyzed the proportion and phenotypic characteristics of peripheral blood Tregs in normal pregnant women, women with recurrent pregnancy loss (RPL) or gestational diabetes mellitus (GDM), and non-pregnant women.Methods: We investigated the proportion of CD4+ Tregs, CD8+ Tregs and the expression of PD-1, GITR, HLA-G and CTLA-4 on them in the peripheral blood of normal pregnancies during 1st (n = 28), 2nd (n = 43), and 3rd trimester (n = 33); In addition, we evaluated pregnancies in the 1st trimester complicated by RPL (n = 21), in the 2nd (n = 17) and 3rd trimester (n = 28) complicated by GDM. Non-pregnant women (n = 57) were also investigated using flow cytometry.Results: During normal pregnancy, the proportion of CD4+ Tregs in all trimester and CD8+ Tregs in 2nd and 3rd trimester were higher(P ＜ 0.05，respectively) compared with non-pregnancy women. Moreover, the proportion of CD4+ Tregs was higher in 2nd trimester compared to 1st and 3rd trimester (P ＜ 0.01) while the proportion of CD8+ Tregs was higher in 3rd trimester compared to 1st and 2nd trimester (P ＜ 0.05). Compared to non-pregnant studies, the proportion of GITR+/CD8+ Tregs and HLA-G+/CD8+ Tregs in all trimester were higher(P ＜ 0.05, respectively). Moreover, the proportion of PD-1+/CD4+ Tregs, GITR+/CD4+ Tregs, PD-1+/CD8+ Tregs and CTLA-4+/CD8+ Tregs in 3rd trimester were significantly higher compared to 1st, 2nd trimester and non-pregnant group(P ＜ 0.05, respectively).In RPL and GDM groups, the proportions of CD4+ Tregs in all trimesters were decreased while the proportions of CD8+ Tregs in all trimesters were increased compared to normal pregnant group (P ＜ 0.05，respectively).In RPL group, the proportion of PD-1+/CD4+ Tregs, GITR+/CD4+ Tregs and HLA-G+/CD4+ Tregs were decreased compared to 1st trimester normal pregnant group (P ＜ 0.05，respectively). In 2nd trimester GDM group, the proportion of HLA-G+/CD4+ Tregs were decreased compared to 2nd trimester normal pregnant group (P ＜ 0.05，respectively). In 3rd trimester GDM group, the proportion of PD-1+/CD4+ Tregs, GITR+/CD4+ Tregs, PD-1+/CD8+ Tregs, GITR+/CD8+ Tregs and HLA-G+/CD8+Tregs were decreased compared to 3rd trimester normal pregnant group (P ＜ 0.05, respectively).Conclusions: The proportion of CD4+ Tregs and CD8+ Tregs increased during pregnancy, the proportions and subsets of CD4+ Tregs decreased and those of CD8+ Tregs increased in pregnancies complicated by RPL and GDM, indicating that regulatory T cells play a role in pregnancy maintenance, and the abnormal expression of Tregs might be related to the complicated pregnancy.

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T cell Subsets in Peripheral Blood of Women with Recurrent Implantation Failure

Cited by 54 publications

References 40 publications

MicroRNAs Regulate Intestinal Immunity and Gut Microbiota for Gastrointestinal Health: A Comprehensive Review

MicroRNAs Regulate Intestinal Immunity and Gut Microbiota for Gastrointestinal Health: A Comprehensive Review

Circulating and Endometrial Regulatory T Cell and Related Populations in Endometriosis and Infertility: Endometriosis Is Associated with Blunting of Endometrial Cyclical Effects and Reduced Proportions in Moderate-Severe Disease

The Fluctuation of Regulatory T Cells during Pregnancy and Obstetrical Complications

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