T Cell Responses to Paraphenylenediamine and to Its Metabolites Mono- and Diacetyl-Paraphenylenediamine

Sieben, Sonja; Kawakubo, Yo; Sachs, B.; Masaoudi, Tlaytmas Al; Blömeke, Brunhilde

doi:10.1159/000053756

Cited by 15 publications

(11 citation statements)

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“…Thus, we first investigated in vitro T‐cell responses to the acetylated PPD derivatives monoacetyl‐PPD (MAPPD) and N , N ′‐diacetyl‐PPD (DAPPD) in 13 PPD‐allergic persons and found no or very limited responses to the acetylated compounds using the lymphocyte transformation test. These results did not change when the assays were performed in the presence of antigen‐modified CYPs (24). We aim to validate our finding in vivo by patch testing with them on patients with eczema.…”

mentioning

confidence: 69%

Elicitation response characteristics to mono‐ and to N,N′‐diacetyl‐para‐phenylenediamine

Blömeke

Pietzsch

2008

Contact Dermatitis

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mentioning

confidence: 69%

Elicitation response characteristics to mono‐ and to N,N′‐diacetyl‐para‐phenylenediamine

Blömeke

Pietzsch

2008

Contact Dermatitis

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“…When N ‐acetylated PPD metabolites, namely monoacetyl‐PPD (MAPPD) and diacetyl‐PPD (DAPPD), were analysed for their capacity to mature human dendritic cells or to induce sensitization in the local lymph node assay, 20 no response was observed. In addition, MAPPD and DAPPD did not reactivate T cells from PPD‐allergic patients in vitro 21 or in vivo 22 . Furthermore, using primary human keratinocytes we also demonstrated that the acetylated compounds are not substrates for the formation of BB, 18 thereby probably reducing the amount of immunogens available for sensitization and allergic reactions.…”

mentioning

confidence: 73%

Para-phenylenediamine and allergic sensitization: risk modification byN-acetyltransferase 1 and 2 genotypes

Blömeke

Brans

Coenraads

et al. 2009

British Journal of Dermatology

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Take-down policy If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.Downloaded from the University of Groningen/UMCG research database (Pure): http://www.rug.nl/research/portal. For technical reasons the number of authors shown on this cover page is limited to 10 maximum. SummaryBackground Para-phenylenediamine (PPD) is a common contact sensitizer causing allergic contact dermatitis, a major skin problem. As PPD may need activation to become immunogenic, the balance between activation and ⁄or detoxification processes may influence an individual's susceptibility. PPD is acetylated and the metabolites do not activate dendritic-like cells and T cells of PPD-sensitized individuals.Objectives To investigate whether PPD can be acetylated in vitro by the two N-acetyltransferases 1 (NAT1) and 2 (NAT2). Based on the assumption that N-acetylation by NAT1 or NAT2 is a detoxification reaction with respect to sensitization, we examined whether NAT1 and NAT2 genotypes are different between PPDsensitized individuals and matched controls. Methods Genotyping for NAT1 and NAT2 polymorphisms was performed in 147 PPD-sensitized individuals and 200 age-and gender-matched controls.Results Both PPD and monoacetyl-PPD were N-acetylated in vitro by recombinant human NAT1 and to a lesser extent by NAT2. Genotyping for NAT1*3, NAT1*4, NAT1*10, NAT1*11 and NAT1*14 showed that genotypes containing the rapid acetylator NAT1*10 allele were under-represented in PPD-sensitized cases (adjusted odds ratio 0AE72, 95% confidence interval 0AE45-1AE16). For NAT2, NAT2*4, NAT2*5AB, NAT2*5C, NAT2*6A and NAT2*7B alleles were genotyped. Individuals homozygous for the rapid acetylator allele NAT2*4 were under-represented in cases compared with controls (4AE3% vs. 9AE4%), but this trend was not significant. Conclusions With respect to data indicating that NAT1 but not NAT2 is present in human skin, we conclude that NAT1 genotypes containing the rapid acetylator NAT1*10 allele are potentially associated with reduced susceptibility to PPD sensitization.

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“…Reports of angioneurotic edema and anaphylaxis exist as well (2). PPD is a potent T-cell stimulator (5). Patch testing patients with PPD shows a reaction consistent with a type IV delayed hypersensitivity reaction.…”

Section: Discussionmentioning

confidence: 99%

Type Iv Hypersensitivity Reaction to a Temporary Tattoo

Sonnen¹

2007

Baylor University Medical Center Proceedings

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T Cell Responses to Paraphenylenediamine and to Its Metabolites Mono- and Diacetyl-Paraphenylenediamine

Cited by 15 publications

References 3 publications

Elicitation response characteristics to mono‐ and to N,N′‐diacetyl‐para‐phenylenediamine

Elicitation response characteristics to mono‐ and to N,N′‐diacetyl‐para‐phenylenediamine

Para-phenylenediamine and allergic sensitization: risk modification byN-acetyltransferase 1 and 2 genotypes

Type Iv Hypersensitivity Reaction to a Temporary Tattoo

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