T-cell repertoire analysis in chronic plaque psoriasis suggests an antigen-specific immune response

Bour, H; Puisieux, Isabelle; Even, Jos; Kourilsky, Philippe; Favrot, M.; Musette, Philippe; Nicolas, Jean‐François

doi:10.1016/s0198-8859(99)00027-0

Cited by 38 publications

(21 citation statements)

References 40 publications

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“…In addition to the important information regarding the nature of such a potent T cell stimulus as SAgs, the finding presented here have vital implications for future strategies in designing drugs aimed to prevent or interfere with the interactions between SAgs and the host receptors. One important consequence is that investigating whether a patient has a skewed variable β-domain repertoire, which is a common way to determine whether a disease is caused by SAg or not [32][33][34] , may be misleading, as our results show that Vβ interaction is not the exclusive means of T cell activation by SAgs. Thus, the results presented here, describing the previously unknown feature of SAgs, activation of T cells through the TCRVα, could have significance for the understanding of these diseases.…”

Section: Discussionmentioning

confidence: 88%

The structure of superantigen complexed with TCR and MHC reveals novel insights into superantigenic T cell activation

et al. 2010

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superantigens (sAgs) are bacterial toxins that interact with immunoreceptors, T cell receptor (TCR) and major histocompatibility complex (mHC) class II, conventionally through the variable β-domain of TCR (TCRVβ). They induce a massive release of cytokines, which can lead to diseases such as food poisoning and toxic shock syndrome. In this study, we report the X-ray structure of the ternary complex between staphylococcal enterotoxin H (sEH) and its human receptors, mHC class II and TCR. The structure demonstrates that sEH predominantly interacts with the variable α-domain of TCR (TCRVα), which is supported by nuclear magnetic resonance (nmR) analyses. Furthermore, there is no contact between mHC and TCR upon complex formation. structural analyses suggest that the major contact points to TCRVα are conserved among other bacterial sAgs. Consequently, a new dimension of sAg biology emerges, suggesting that in addition to the conventional interactions with the TCRVβ domain, sAgs can also activate T cells through the TCRVα domain.

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Section: Discussionmentioning

confidence: 88%

The structure of superantigen complexed with TCR and MHC reveals novel insights into superantigenic T cell activation

et al. 2010

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“…16 Skewing of the T-cell VB spectrum has also been described for animal models of immunologically mediated diseases such as encephalitis 17,18 and thyroiditis. 19 In humans, expansion of specific CDR3 VB TCR clones has been found in type I diabetes mellitus, 20 thyroiditis, 21 rheumatoid arthritis, [22][23][24] primary biliary cirrhosis, 25 psoriasis, 26,27 or during graft-versus-host disease. 28 TCR VB repertoire analysis has also been performed in patients with AA, 12,13 myelodysplasia (MDS), 29 and paroxysmal nocturnal hemoglobinuria (PNH).…”

Section: Introductionmentioning

confidence: 99%

Changes in T-cell receptor VB repertoire in aplastic anemia: effects of different immunosuppressive regimens

Kook

Risitano

Zhang

et al. 2002

Blood

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We studied the degree and the pattern of skewing of the variable region of ␤-chain (VB) T-cell receptor (TCR) repertoire in aplastic anemia (AA) at initial presentation and after immunosuppression using a high-resolution analysis of the TCR VB complementarity-determining region 3 (CDR3). Age-matched healthy individuals and multitransfused patients with nonimmune-mediated hematologic diseases were used as controls. In newly diagnosed AA, the average frequency of CDR3 size distribution deviation indicative of oligoclonal T-cell proliferation was increased (44% ؎ 33% vs 9% ؎ 9%; P ‫؍‬ .0001); AA patients with human leukocyte antigen (HLA)-DR2 and those with expanded paroxysmal nocturnal hemoglobinuria clones showed more skewed VB repertoires. Nonrandom oligoclonal patterns were found for VB6, VB14-16, VB21, VB23, and VB24 subfamilies in more than 50%, and for VB15, VB21, and VB24 in more than 70% of AA patients with HLA-DR2. Patients received immunosuppression with antithymocyte globulin (ATG)/ cyclosporine (CsA) or cyclophosphamide (CTX) with CsA in combination, and their VB repertoire was reanalyzed after treatment. Whereas no significant change in the degree of VB skewing in patients who had received ATG was seen, patients treated with CTX showed a much higher extent of oligoclonality within all VB families, consistent with a profound and longlasting contraction of the T-cell repertoire. VB analysis did not correlate with the lymphocyte count prior to lymphocytotoxic therapy; however, after therapy the degree of VB skewing was highly reflective of the decrease in lymphocyte numbers, suggesting iatrogenic gaps in the VB repertoire rather than the emergence of clonal dominance. Our data indicate that multiple specific clones mediate the immune process in AA. (Blood. 2002; 99:3668-3675)

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“…This inconsistency has been reported by other authors, [20] and could be explained by the event referred to as the Koebner response [12]. Previous studies have reported selectivity of the clones displaying different TCR Vβ of infiltrated T-cells in psoriatic lesions: TCR Vβ-13 and -15 [11], TCR Vβ-5.1, -11, -12, -13.1 and -16 [21], TCR Vβ-3 and -13.1 [10], and more recently TCR Vβ-3, -13S2 and -21 [22]. This evidence is bound to studies of patient specimens with streptococcal infections.…”

Section: Discussionmentioning

confidence: 80%

“…The hypervariable region of the TCR Vβ gene family was examined as reported by several authors [10,13,14] ( Table 3). In a subset of regions within group 1, the Vβ2 and Vβ7 subfamilies were predominantly expressed.…”

Section: Tcr Vβ Expressionmentioning

confidence: 99%

“…Superantigens, unlike conventional antigens, activate T-cells expressing certain T-cell receptors, which possess a highly variable region known as the variable β region (TCR Vβ). The importance of this region and its role in autoimmune diseases has been determined by Bour et al [10], although several authors have found differences in the expression of the TCR Vβ activity for the presentation of antigens to the MHC class II on antigen presenting cells [11][12][13]. Preferential usage of certain T-cell receptors by the lymphocytic infiltrate in psoriasis might indicate the involvement of one or several antigens in the pathogenesis of psoriasis.…”

Section: Introductionmentioning

confidence: 99%

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HLA-Cw and TCR vβ analysis in twenty Mexican patients with psoriasis

et al. 2011

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AbstractGenetic background and T-cell expansion have been confirmed as the most important factors leading to psoriasis susceptibility in the Caucasian population. This study was performed to identify the T-cell receptor Vβ repertoire and HLA-Cw genotype in twenty Mexicans of two different ethnicities with severe chronic plaque-type psoriasis. HLA-Cw typing was performed to detect the allele pattern by SSP-PCR. In parallel, RT-PCR and Western blot were used for the identification of the TCR Vβ expression in peripheral blood cells. We identified a variety of HLA-Cw alleles in this group of patients distinct from the widely known HLA-Cw 0602 Caucasian allele. Moreover, TCR Vβ-2 and Vβ-7 clone-type frequencies were different and statistically significant (P = 0.0280). We speculate that because of diverse genetic backgrounds, the susceptibility to disease and activation of T-cells for a proper immune response could be specific; therefore, the findings might contribute to the elucidation of the pathogenesis in psoriatic Mexican patients.

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T-cell repertoire analysis in chronic plaque psoriasis suggests an antigen-specific immune response

Cited by 38 publications

References 40 publications

The structure of superantigen complexed with TCR and MHC reveals novel insights into superantigenic T cell activation

The structure of superantigen complexed with TCR and MHC reveals novel insights into superantigenic T cell activation

Changes in T-cell receptor VB repertoire in aplastic anemia: effects of different immunosuppressive regimens

HLA-Cw and TCR vβ analysis in twenty Mexican patients with psoriasis

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